Slc17a9-flox Mouse
一般名
Slc17a9-flox
製品ID
S-CKO-07222
背景情報
C57BL/6JCya
系統ID
CKOCMP-228993-Slc17a9-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Slc17a9-flox Mouse（カタログ番号S-CKO-07222）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Slc17a9-flox
系統ID
CKOCMP-228993-Slc17a9-B6J-VA
遺伝子名
製品ID
S-CKO-07222
遺伝子別名
1700019H03Rik, Vnut
遺伝子別名
C57BL/6JCya
遺伝子正式名称
solute carrier family 17, member 9
NCBI ID
修正
Conditional knockout
染色体
Chr 2
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000094218
NCBIトランスクリプトID
NM_183161
ターゲット領域
Exon 2~3
有効領域の大きさ
~1.2 kb
遺伝子研究の概要
Slc17a9, also known as VNUT (vesicular nucleotide transporter), is a transmembrane protein encoding a vesicular ATP transport protein. It functions in lysosomes and other secretory vesicles, playing a crucial role in maintaining lysosomal function and cell viability, and is involved in multiple biological processes. It has been associated with pathways like the PI3K/Akt signaling pathway [3].
In cancer research, Slc17a9 has shown significant implications. In clear cell renal cell carcinoma (ccRCC), its knockdown inhibited cancer cell proliferation, migration, and invasion, while overexpression promoted a more malignant phenotype both in vitro and in vivo. It was found to upregulate PTHLH, promoting epithelial-mesenchymal transition (EMT) in ccRCC [1]. In hepatocellular carcinoma (HCC), the HHEX-ABI2/Slc17a9 axis was shown to induce cancer stem cell-like properties and tumorigenesis [2]. Additionally, in non-small cell lung cancer (NSCLC), its up-regulation was correlated with survival, and knockdown significantly inhibited cell proliferation and ATP levels in lung cancer cells [5]. In gastric, colorectal cancers, high Slc17a9 expression was associated with poor survival, suggesting it could be a prognostic biomarker [4,8]. In osteoblasts, knockdown of Slc17a9 reduced extracellular ATP levels and led to increased osteoblast differentiation in response to compressive force [6]. In the context of heart-brain interaction during pressure overload, adrenergic neuron-specific knockout of Slc17a9 reduced cardiac extracellular ATP level, inhibited NLRP3 inflammasome activation, IL-1β production, and adaptive cardiac hypertrophy [7].
In conclusion, Slc17a9 is essential for maintaining cell viability through regulating lysosomal function. Its role in multiple cancer types, osteoblast differentiation, and cardiac inflammation and hypertrophy has been revealed through various gene-knockout and knockdown experiments. These findings suggest that Slc17a9 could be a potential therapeutic target and prognostic biomarker in related diseases.
References：
1. Li, Weiquan, Xu, Ning, Meng, Xiangui, Xiao, Wen, Zhang, Xiaoping. 2022. SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma. In iScience, 26, 105764. doi:10.1016/j.isci.2022.105764. https://pubmed.ncbi.nlm.nih.gov/36590170/
2. Li, Huizi, Liu, Jin, Lai, Jie, Zhang, Tong, Gu, Qiuping. 2024. The HHEX-ABI2/SLC17A9 axis induces cancer stem cell-like properties and tumorigenesis in HCC. In Journal of translational medicine, 22, 537. doi:10.1186/s12967-024-05324-2. https://pubmed.ncbi.nlm.nih.gov/38844969/
3. Kui, Xue-Yan, Gao, Yan, Liu, Xu-Sheng, Zhang, Yao-Hua, Pei, Zhi-Jun. 2022. Comprehensive Analysis of SLC17A9 and Its Prognostic Value in Hepatocellular Carcinoma. In Frontiers in oncology, 12, 809847. doi:10.3389/fonc.2022.809847. https://pubmed.ncbi.nlm.nih.gov/35957868/
4. Li, Junqing, Su, Taiqiang, Yang, Liang, Zhang, Changhua, He, Yulong. 2019. High SLC17A9 expression correlates with poor survival in gastric carcinoma. In Future oncology (London, England), 15, 4155-4166. doi:10.2217/fon-2019-0283. https://pubmed.ncbi.nlm.nih.gov/31799885/
5. Gao, Yan, Chen, Yijia, Liu, Min, Ke, Changbin, Pei, Zhijun. 2023. SLC17A9 as a prognostic biomarker correlated with immune infiltrates in human non-small cell lung cancer. In American journal of cancer research, 13, 3963-3982. doi:. https://pubmed.ncbi.nlm.nih.gov/37818081/
6. Inoue, Asako, Nakao-Kuroishi, Kayoko, Kometani-Gunjigake, Kaori, Kokabu, Shoichiro, Kawamoto, Tatsuo. 2020. VNUT/SLC17A9, a vesicular nucleotide transporter, regulates osteoblast differentiation. In FEBS open bio, 10, 1612-1623. doi:10.1002/2211-5463.12918. https://pubmed.ncbi.nlm.nih.gov/32592329/
7. Higashikuni, Yasutomi, Liu, Wenhao, Numata, Genri, Komuro, Issei, Sata, Masataka. 2022. NLRP3 Inflammasome Activation Through Heart-Brain Interaction Initiates Cardiac Inflammation and Hypertrophy During Pressure Overload. In Circulation, 147, 338-355. doi:10.1161/CIRCULATIONAHA.122.060860. https://pubmed.ncbi.nlm.nih.gov/36440584/
8. Yang, Liang, Chen, Zhihui, Xiong, Weixin, Song, Xinming, Liu, Jia. 2018. High expression of SLC17A9 correlates with poor prognosis in colorectal cancer. In Human pathology, 84, 62-70. doi:10.1016/j.humpath.2018.09.002. https://pubmed.ncbi.nlm.nih.gov/30236596/
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