Zeb2-flox Mouse
一般名
Zeb2-flox
製品ID
S-CKO-08365
背景情報
C57BL/6JCya
系統ID
CKOCMP-24136-Zeb2-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Zeb2-flox Mouse（カタログ番号S-CKO-08365）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Zeb2-flox
系統ID
CKOCMP-24136-Zeb2-B6J-VA
遺伝子名
製品ID
S-CKO-08365
遺伝子別名
9130203F04Rik, D130016B08Rik, SIP1, Zfhx1b, Zfx1b, Zfxh1b
遺伝子別名
C57BL/6JCya
遺伝子正式名称
zinc finger E-box binding homeobox 2
NCBI ID
修正
Conditional knockout
染色体
Chr 2
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000068415
NCBIトランスクリプトID
NM_015753
ターゲット領域
Exon 6
有効領域の大きさ
~1.2 kb
遺伝子研究の概要
Zeb2, also known as Sip1 and Zfhx1b, is a zinc finger E-box binding homeobox transcription factor. It is a master regulator of cellular plasticity involved in multiple biological processes, such as neuronal development, hematopoiesis, and the immune response [4,5,7]. In the context of the immune system, it has been associated with pathways related to B-cell and T-cell development and function, as well as autoimmune disease pathogenesis [1,2,3]. Mouse models, including gene knockout (KO) and conditional knockout (CKO) models, have been crucial in understanding Zeb2's functions in vivo.
In B-cell research, Zeb2 has been shown to be essential for the differentiation of age-associated B cells (ABCs) in both humans and mice. Zeb2 haploinsufficient individuals and mice lacking Zeb2 in B cells have reduced ABCs. In TLR7-driven lupus mouse models, Zeb2 is necessary for ABC formation and autoimmune pathology. Zeb2 binds to the +20-kb myocyte enhancer factor 2b (Mef2b)'s intronic enhancer, repressing MEF2B-mediated germinal center B cell differentiation and promoting ABC formation. It also targets genes important for ABC specification and function, like Itgax [1]. Similarly, in another study, Zeb2 was found to be required for the formation of CD11c+ atypical B cells in mice, which are important for controlling persistent infection by sustaining germinal center responses. Zeb2 haplo-insufficient Mowat-Wilson syndrome (MWS) patients have decreased circulating ABCs [6]. In CD4+ T cells, an age-associated T helper (THA) cell subset's B-cell-promoting functions are regulated by Zeb2 in autoimmunity [3].
In conclusion, Zeb2 is a multifunctional transcription factor with key roles in neuronal development, hematopoiesis, and immune-related processes. KO and CKO mouse models have been instrumental in revealing its function in autoimmune diseases, specifically in relation to the development and function of B cells and certain T-cell subsets, highlighting its potential as a therapeutic target in these disease areas [1,3,6].
References：
1. Dai, Dai, Gu, Shuangshuang, Han, Xiaxia, Vinuesa, Carola G, Shen, Nan. 2024. The transcription factor ZEB2 drives the formation of age-associated B cells. In Science (New York, N.Y.), 383, 413-421. doi:10.1126/science.adf8531. https://pubmed.ncbi.nlm.nih.gov/38271512/
2. De Coninck, Stien, Berx, Geert, Taghon, Tom, Van Vlierberghe, Pieter, Goossens, Steven. 2019. ZEB2 in T-cells and T-ALL. In Advances in biological regulation, 74, 100639. doi:10.1016/j.jbior.2019.100639. https://pubmed.ncbi.nlm.nih.gov/31383581/
3. Goto, Manaka, Takahashi, Hideyuki, Yoshida, Ryochi, Okamura, Tomohisa, Fujio, Keishi. 2024. Age-associated CD4+ T cells with B cell-promoting functions are regulated by ZEB2 in autoimmunity. In Science immunology, 9, eadk1643. doi:10.1126/sciimmunol.adk1643. https://pubmed.ncbi.nlm.nih.gov/38330141/
4. Epifanova, Ekaterina, Babaev, Alexey, Newman, Andrew G, Tarabykin, Victor. 2018. Role of Zeb2/Sip1 in neuronal development. In Brain research, 1705, 24-31. doi:10.1016/j.brainres.2018.09.034. https://pubmed.ncbi.nlm.nih.gov/30266271/
5. Birkhoff, Judith C, Huylebroeck, Danny, Conidi, Andrea. 2021. ZEB2, the Mowat-Wilson Syndrome Transcription Factor: Confirmations, Novel Functions, and Continuing Surprises. In Genes, 12, . doi:10.3390/genes12071037. https://pubmed.ncbi.nlm.nih.gov/34356053/
6. Gao, Xin, Shen, Qian, Roco, Jonathan A, Zaunders, John J, Cockburn, Ian A. 2024. Zeb2 drives the formation of CD11c+ atypical B cells to sustain germinal centers that control persistent infection. In Science immunology, 9, eadj4748. doi:10.1126/sciimmunol.adj4748. https://pubmed.ncbi.nlm.nih.gov/38330097/
7. Hegarty, Shane V, Sullivan, Aideen M, O'Keeffe, Gerard W. 2015. Zeb2: A multifunctional regulator of nervous system development. In Progress in neurobiology, 132, 81-95. doi:10.1016/j.pneurobio.2015.07.001. https://pubmed.ncbi.nlm.nih.gov/26193487/
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凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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