Rasef-flox Mouse
一般名
Rasef-flox
製品ID
S-CKO-08442
背景情報
C57BL/6JCya
系統ID
CKOCMP-242505-Rasef-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Rasef-flox Mouse（カタログ番号S-CKO-08442）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Rasef-flox
系統ID
CKOCMP-242505-Rasef-B6J-VA
遺伝子名
製品ID
S-CKO-08442
遺伝子別名
RAB45
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 4
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000058292
NCBIトランスクリプトID
NM_001017427
ターゲット領域
Exon 3~4
有効領域の大きさ
~2.6 kb
遺伝子研究の概要
RASEF, also known as Rab45, is an atypical Rab GTPase. It contains N-terminal EF-hand and coiled-coil domains, along with a C-terminal Rab-GTPase domain. It functions as a key regulator in membrane trafficking, and has been associated with processes like DNA repair, and may play important roles in various biological and disease-related pathways [2,7]. Genetic models, such as knockout mice, are valuable for studying its functions in vivo.
In pancreatic acinar cells, RASEF-knockout (KO) mice studies revealed that RASEF likely regulates the formation and sorting of zymogen granules and secretion of digestive enzymes. Pancreatic acinar cells in RASEF-KO mice had an increased number of zymogen granules, abnormal organelle formations, and impaired enzyme release upon cerulein administration [2].
In the context of diseases, in colorectal cancer, high RASEF expression was associated with a better prognosis, and it may act as a suppressor gene, potentially inhibiting cancer development through participation in DNA repair processes [1]. In breast cancer, RASEF mRNA expression levels were associated with hormone receptor status, with lower expression in patients with negative hormone receptor status [3]. In pulmonary hypertension rats, cigarette smoke-induced RASEF hypermethylation led to pulmonary arterial smooth muscle remodeling, and RASEF overexpression inhibited this remodeling [4]. In lung cancer, elevated RASEF promoted cell growth via enhanced ERK signaling and was associated with poor prognosis [5]. In uveal melanoma, low RASEF expression correlated with promoter methylation and was associated with decreased survival, suggesting a tumor-suppressor role [6].
In conclusion, RASEF plays essential roles in multiple biological processes, including membrane trafficking in pancreatic acinar cells. Its functions in disease are diverse, with implications in cancer prognosis and development, as well as in pulmonary arterial remodeling. Studies using RASEF KO mouse models have provided valuable insights into its role in these specific disease areas.
References：
1. Yu, Xin, Fang, Zhenhuan, Li, Guodong, Liu, Ming, Wang, Ying. 2018. High RASEF expression is associated with a significantly better prognosis in colorectal cancer. In International journal of clinical and experimental pathology, 11, 4276-4282. doi:. https://pubmed.ncbi.nlm.nih.gov/31949824/
2. Sato, Keiko, Kadowaki, Tomoko, Takenaka, Mamoru, Onodera, Takae, Tsukuba, Takayuki. 2024. RASEF/Rab45 regulates the formation and sorting of zymogen granules and secretion of digestive enzymes by pancreatic acinar cells. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167310. doi:10.1016/j.bbadis.2024.167310. https://pubmed.ncbi.nlm.nih.gov/38901651/
3. Shibata, Masahiro, Kanda, Mitsuro, Shimizu, Dai, Kodera, Yasuhiro, Kikumori, Toyone. 2018. RASEF expression correlates with hormone receptor status in breast cancer. In Oncology letters, 16, 7223-7230. doi:10.3892/ol.2018.9542. https://pubmed.ncbi.nlm.nih.gov/30546460/
4. Li, Qinghai, Wu, Jixing, Xu, Yongjian, Liu, Lu, Xie, Jungang. 2019. Role of RASEF hypermethylation in cigarette smoke-induced pulmonary arterial smooth muscle remodeling. In Respiratory research, 20, 52. doi:10.1186/s12931-019-1014-1. https://pubmed.ncbi.nlm.nih.gov/30845941/
5. Oshita, Hideto, Nishino, Ryohei, Takano, Atsushi, Nakamura, Yusuke, Daigo, Yataro. 2013. RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer. In Molecular cancer research : MCR, 11, 937-51. doi:10.1158/1541-7786.MCR-12-0685-T. https://pubmed.ncbi.nlm.nih.gov/23686708/
6. Maat, Willem, Beiboer, Sigrid H W, Jager, Martine J, Gruis, Nelleke A, van der Velden, Pieter A. . Epigenetic regulation identifies RASEF as a tumor-suppressor gene in uveal melanoma. In Investigative ophthalmology & visual science, 49, 1291-8. doi:10.1167/iovs.07-1135. https://pubmed.ncbi.nlm.nih.gov/18385040/
7. Shintani, Mami, Tada, Minoru, Kobayashi, Tetsuo, Kontani, Kenji, Katada, Toshiaki. 2007. Characterization of Rab45/RASEF containing EF-hand domain and a coiled-coil motif as a self-associating GTPase. In Biochemical and biophysical research communications, 357, 661-7. doi:. https://pubmed.ncbi.nlm.nih.gov/17448446/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。