Wasf2-flox Mouse
一般名
Wasf2-flox
製品ID
S-CKO-08467
背景情報
C57BL/6NCya
系統ID
CKOCMP-242687-Wasf2-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Wasf2-flox Mouse（カタログ番号S-CKO-08467）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Wasf2-flox
系統ID
CKOCMP-242687-Wasf2-B6N-VA
遺伝子名
製品ID
S-CKO-08467
遺伝子別名
WAVE2, D4Ertd13e
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conditional knockout
染色体
Chr 4
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000084241
NCBIトランスクリプトID
NM_153423
ターゲット領域
Exon 5
有効領域の大きさ
~0.6 kb
遺伝子研究の概要
WASF2, also known as Wiskott-Aldrich syndrome protein Verprolin-homologous protein 2 or WAVE2, is an integral member of the actin cytoskeleton pathway, playing a crucial role in cell motility, such as regulating actin reorganization during cell migration [1,3]. It is involved in multiple signaling pathways, including TGF-β signaling, Kras signaling, fatty acid metabolism, p53 pathways, and Wnt/β-catenin axis [1,4]. Genetic models can be valuable for studying its functions.
In various cancers, WASF2 shows different roles. In head and neck squamous cell carcinoma (HNSCC), hypermethylated WASF2 acts as a tumor suppressor. Its down-regulation in HNSCC tissues is related to elevated promoter methylation, and DNA methylation inhibitor can restore its mRNA expression. WASF2 expression is a favorable indicator for HPV-positive HNSCC, and its promoter methylation is detrimental to patient survival. Also, it is positively associated with tumor-killing immune cells [1]. In ovarian cancer, WASF2 is abnormally expressed, significantly correlated with overall survival and progression-free interval, and related to the tumor microenvironment, immune infiltration, TMB, MSI, and antitumor drugs sensitivity. Knockdown of WASF2 inhibits the proliferative, migratory and invasive capacity of ovarian cancer cells [2]. In hepatocellular carcinoma (HCC), WASF2 is overexpressed and hypomethylated, and its inactivation exerts anti-tumorigenic effects on HCC cells in vitro and in vivo [3]. In spinal cord glioma, EMX1 functions as a tumor inhibitor by suppressing WASF2-dependent activation of the Wnt/β-catenin axis [4].
In conclusion, WASF2 is essential in regulating cell motility through the actin cytoskeleton pathway. Its abnormal expression and epigenetic changes are closely associated with the development and prognosis of multiple cancers, such as HNSCC, ovarian cancer, HCC, and spinal cord glioma. Studies using various models help to understand its functions in these disease conditions, providing potential targets for cancer treatment.
References：
1. Zhang, Jianyun, Ding, Zhuang, Chen, Long, Qin, Haiyan. 2022. Hypermethylated WASF2: tumor suppressive role in head and neck squamous cell carcinoma. In Translational cancer research, 12, 78-92. doi:10.21037/tcr-22-1133. https://pubmed.ncbi.nlm.nih.gov/36760387/
2. Yang, Xiaofeng, Ding, Yuzhen, Sun, Lu, Huang, Zhengrui, Li, Ruiman. 2022. WASF2 Serves as a Potential Biomarker and Therapeutic Target in Ovarian Cancer: A Pan-Cancer Analysis. In Frontiers in oncology, 12, 840038. doi:10.3389/fonc.2022.840038. https://pubmed.ncbi.nlm.nih.gov/35359421/
3. Ahn, Hye Ri, Baek, Geum Ok, Yoon, Moon Gyeong, Eun, Jung Woo, Kim, Soon Sun. 2022. Hypomethylation-mediated upregulation of the WASF2 promoter region correlates with poor clinical outcomes in hepatocellular carcinoma. In Journal of experimental & clinical cancer research : CR, 41, 158. doi:10.1186/s13046-022-02365-7. https://pubmed.ncbi.nlm.nih.gov/35477411/
4. Han, Ziyin, Mou, Zufang, Jing, Yulong, Jiang, Rong, Sun, Tao. 2022. EMX1 functions as a tumor inhibitor in spinal cord glioma through transcriptional suppression of WASF2 and inactivation of the Wnt/β-catenin axis. In Brain and behavior, 12, e2684. doi:10.1002/brb3.2684. https://pubmed.ncbi.nlm.nih.gov/35849030/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
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