Dhrs4-flox Mouse
一般名
Dhrs4-flox
製品ID
S-CKO-10150
背景情報
C57BL/6JCya
系統ID
CKOCMP-28200-Dhrs4-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Dhrs4-flox Mouse（カタログ番号S-CKO-10150）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Dhrs4-flox
系統ID
CKOCMP-28200-Dhrs4-B6J-VA
遺伝子名
製品ID
S-CKO-10150
遺伝子別名
CR, RRD, NDRD, PHCR, PSCD, mouNRDR, D14Ucla2
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 14
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000022821
NCBIトランスクリプトID
NM_001037938
ターゲット領域
Exon 2
有効領域の大きさ
~1.0 kb
遺伝子研究の概要
DHRS4, a gene within the human DHRS4 gene cluster which also includes DHRS4L2 and DHRS4L1, encodes an NADP(H)-dependent retinol dehydrogenase/reductase [9]. It has been implicated in various biological processes, and understanding its function is crucial for elucidating related physiological and pathological mechanisms.
In cancer research, DHRS4-AS1, an antisense lncRNA associated with DHRS4, has been extensively studied. In gastric cancer, its down-regulation is linked to tumor size, advanced stage, and vascular invasion. It inhibits cell proliferation and promotes apoptosis by destabilizing pro-oncogenic DHX9 and disrupting its association with ILF3 [1]. In hepatocellular carcinoma, DHRS4-AS1 is down-regulated, and its overexpression restrains cell proliferation, promotes apoptosis, and arrests the cell cycle in G0/G1 phase via the miR-522-3p/SOCS5 axis [2]. In NSCLC, DHRS4-AS1 suppresses cancer cell stemness by sponging miR-224-3p and upregulating TP53 and TET1 [3]. In endometriosis, DHRS4-AS1 inhibits ectopic endometrial cell proliferation, migration, and invasion by regulating miR-139-5p expression [6]. In thyroid cancer, DHRS4-AS1 acts as a miR-222-3p sponge, and its overexpression inhibits cell proliferation and promotes apoptosis [7]. In gliomas, miR-29a-5p suppresses cell proliferation, invasion, and migration by targeting DHRS4, suggesting DHRS4 may be an oncogene [8]. In ulcerative colitis, METTL14 regulates inflammation via the DHRS4-AS1/miR-206/A3AR axis, with METTL14 knockdown reducing DHRS4-AS1 expression [4]. In amyotrophic lateral sclerosis, Dhrs4 expression in the spinal cord is upregulated during disease progression, and its upregulation with DHRS3 may be associated with activation of the complement cascade in the immune system [5].
In conclusion, research on DHRS4 and its associated lncRNA DHRS4-AS1 has revealed their significant roles in multiple diseases, especially in cancer-related biological processes such as cell proliferation, apoptosis, and stemness, as well as in neurodegenerative and inflammatory diseases. These findings contribute to a better understanding of disease mechanisms and may offer potential therapeutic targets.
References：
1. Xiao, Lei, Zhang, Yang, Luo, Qingqing, Chen, Zihua, Lai, Chen. 2023. DHRS4-AS1 regulate gastric cancer apoptosis and cell proliferation by destabilizing DHX9 and inhibited the association between DHX9 and ILF3. In Cancer cell international, 23, 304. doi:10.1186/s12935-023-03151-x. https://pubmed.ncbi.nlm.nih.gov/38041141/
2. Zhou, Yongping, Li, Kuan, Zou, Xuexia, Chen, Fangming, Dai, Tu. . LncRNA DHRS4-AS1 ameliorates hepatocellular carcinoma by suppressing proliferation and promoting apoptosis via miR-522-3p/SOCS5 axis. In Bioengineered, 12, 10862-10877. doi:10.1080/21655979.2021.1994719. https://pubmed.ncbi.nlm.nih.gov/34666613/
3. Yan, Fei, Zhao, Wei, Xu, Xiaoyue, Shi, Lin, Wu, Yuan. 2020. LncRNA DHRS4-AS1 Inhibits the Stemness of NSCLC Cells by Sponging miR-224-3p and Upregulating TP53 and TET1. In Frontiers in cell and developmental biology, 8, 585251. doi:10.3389/fcell.2020.585251. https://pubmed.ncbi.nlm.nih.gov/33425890/
4. Wu, Weiyun, Li, Xiaowen, Zhou, Zhuliang, Ye, Shicai, Quan, Juan-Hua. 2024. METTL14 regulates inflammation in ulcerative colitis via the lncRNA DHRS4-AS1/miR-206/A3AR axis. In Cell biology and toxicology, 40, 95. doi:10.1007/s10565-024-09944-8. https://pubmed.ncbi.nlm.nih.gov/39528760/
5. Li, Shu, Zhu, Yu, Wei, Caihui, Yuan, Dongxiang, Xu, Renshi. 2022. Identification of Molecular Correlations Between DHRS4 and Progressive Neurodegeneration in Amyotrophic Lateral Sclerosis By Gene Co-Expression Network Analysis. In Frontiers in immunology, 13, 874978. doi:10.3389/fimmu.2022.874978. https://pubmed.ncbi.nlm.nih.gov/35479082/
6. Cui, Xuan, Zhou, Shisan, Lin, Yongtao. . Long non-coding RNA DHRS4 antisense RNA 1 inhibits ectopic endometrial cell proliferation, migration, and invasion in endometriosis by regulating microRNA-139-5p expression. In Bioengineered, 13, 9792-9804. doi:10.1080/21655979.2022.2060781. https://pubmed.ncbi.nlm.nih.gov/35414313/
7. Xu, Shuai, Zheng, Xinyi, Wu, Haibin, Liu, Qianqian, Ye, Ren-Qun. 2024. Feasibility study of lncRNA DHRS4-AS1 sponge miR-222-3p in the diagnosis of thyroid cancer. In Endokrynologia Polska, 75, 494-500. doi:10.5603/ep.99456. https://pubmed.ncbi.nlm.nih.gov/39376175/
8. Dai, Yong, Chen, Zhenhua, Zhao, Wei, Hu, Hongkang, Zhang, Yi. 2020. miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4. In Frontiers in oncology, 10, 1772. doi:10.3389/fonc.2020.01772. https://pubmed.ncbi.nlm.nih.gov/33014873/
9. Su, Zhong-Jing, Zhang, Qiao-Xia, Liu, Ge-Fei, Sui, Xu-Xia, Huang, Dong-Yang. 2010. Bioinformatic analysis of the human DHRS4 gene cluster and a proposed mechanism for its transcriptional regulation. In BMC molecular biology, 11, 43. doi:10.1186/1471-2199-11-43. https://pubmed.ncbi.nlm.nih.gov/20525226/
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