Scmh1-flox Mouse
一般名
Scmh1-flox
製品ID
S-CKO-10190
背景情報
C57BL/6JCya
系統ID
CKOCMP-29871-Scmh1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Scmh1-flox Mouse（カタログ番号S-CKO-10190）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Scmh1-flox
系統ID
CKOCMP-29871-Scmh1-B6J-VA
遺伝子名
製品ID
S-CKO-10190
遺伝子別名
Scml1, Scml3
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 4
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000000087
NCBIトランスクリプトID
NM_013883
ターゲット領域
Exon 7
有効領域の大きさ
~1.4 kb
遺伝子研究の概要
Scmh1, as a substoichiometric component of Polycomb-group (PcG) complex 1, has E3 ubiquitin ligase activity for geminin and histone H2A. It regulates geminin stability either directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4, which are direct targets of PcG complex 1-mediated transcriptional silencing in hematopoietic cells [6].
Circular RNA SCMH1 (circSCMH1), a product related to the Scmh1 gene, has shown potential in multiple disease-related studies. In ischemic stroke, circSCMH1 levels are decreased in patients' plasma. Treating rodent and nonhuman primate ischemic stroke models with engineered extracellular vesicles delivering circSCMH1 promotes functional recovery, enhances neuronal plasticity, and inhibits glial activation and peripheral immune cell infiltration. It binds to the transcription factor MeCP2, releasing repression of MeCP2 target gene transcription [1]. In addition, circSCMH1 also promotes vascular repair in the peri-infarct cortex of male mice and monkeys after stroke by regulating FTO-dependent m6A modification of Plpp3 [2], and suppresses KMO expression to inhibit mitophagy and promote functional recovery following stroke [3]. In other diseases, circSCMH1 in high-glucose-treated ARPE-19 cells promotes diabetes-induced retinal epithelial-mesenchymal transition by regulating the miR-200a-3p/ZEB1 signaling axis [4], and in oral squamous cell carcinoma, circ-SCMH1 contributes to cis-dichlorodiammineplatinum (DDP) resistance via the circ-SCMH1/miR-338-3p/LIN28B axis [5]. Also, in Parkinson's disease, hsa-SCMH1_0001, a circular RNA, may be involved in the pathogenesis [7], and in traumatic brain injury, exosomes from adipose-derived stem cells delivering circ-Scmh1 can promote microglial M2 polarization and ameliorate hippocampal nerve damage [8].
In summary, Scmh1 and its related circular RNAs play essential roles in various biological processes and disease conditions. The study of Scmh1-related circular RNAs in different disease models, especially in ischemic stroke, diabetes-induced retinopathy, oral squamous cell carcinoma, Parkinson's disease, and traumatic brain injury, has provided insights into disease mechanisms and potential therapeutic targets.
References：
1. Yang, Li, Han, Bing, Zhang, Zhiting, Wang, Jianhong, Yao, Honghong. 2020. Extracellular Vesicle-Mediated Delivery of Circular RNA SCMH1 Promotes Functional Recovery in Rodent and Nonhuman Primate Ischemic Stroke Models. In Circulation, 142, 556-574. doi:10.1161/CIRCULATIONAHA.120.045765. https://pubmed.ncbi.nlm.nih.gov/32441115/
2. Li, Bin, Xi, Wen, Bai, Ying, Han, Bing, Yao, Honghong. 2023. FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke. In Nature communications, 14, 489. doi:10.1038/s41467-023-36008-y. https://pubmed.ncbi.nlm.nih.gov/36717587/
3. Wang, Yu, Bai, Ying, Cai, Yang, Han, Bing, Yao, Honghong. 2024. Circular RNA SCMH1 suppresses KMO expression to inhibit mitophagy and promote functional recovery following stroke. In Theranostics, 14, 7292-7308. doi:10.7150/thno.99323. https://pubmed.ncbi.nlm.nih.gov/39659575/
4. He, Ye, Hou, Xiang-Tao, Guan, Yu-Xin, Chen, Yao. 2022. CircRNA SCMH1 regulates the miR-200a-3p/ZEB1 signaling axis to promote diabetes-induced retinal epithelial-mesenchymal transition. In Experimental eye research, 224, 109264. doi:10.1016/j.exer.2022.109264. https://pubmed.ncbi.nlm.nih.gov/36162459/
5. Qiu, Feng, Qiao, Bin, Zhang, Nan, Zhang, Shanfeng, Qiu, Weiliu. 2021. Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B. In Cancer cell international, 21, 412. doi:10.1186/s12935-021-02110-8. https://pubmed.ncbi.nlm.nih.gov/34353342/
6. Yasunaga, Shin'ichiro, Ohtsubo, Motoaki, Ohno, Yoshinori, Miyoshi, Jun, Takihara, Yoshihiro. 2012. Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4. In Molecular and cellular biology, 33, 644-60. doi:10.1128/MCB.00974-12. https://pubmed.ncbi.nlm.nih.gov/23207902/
7. Wang, Qiao, Wang, Huizhi, Zhao, Xuemin, Cai, Guoen, Meng, Fangang. 2023. Transcriptome sequencing of circular RNA reveals the involvement of hsa-SCMH1_0001 in the pathogenesis of Parkinson's disease. In CNS neuroscience & therapeutics, 30, e14435. doi:10.1111/cns.14435. https://pubmed.ncbi.nlm.nih.gov/37664885/
8. Chen, Songyu, Wang, Xuewei, Qian, Zhouqi, Li, Lei, Gao, Liang. 2023. Exosomes from ADSCs ameliorate nerve damage in the hippocampus caused by post traumatic brain injury via the delivery of circ-Scmh1 promoting microglial M2 polarization. In Injury, 54, 110927. doi:10.1016/j.injury.2023.110927. https://pubmed.ncbi.nlm.nih.gov/37544863/
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精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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