Cpsf6-flox Mouse
一般名
Cpsf6-flox
製品ID
S-CKO-11219
背景情報
C57BL/6NCya
系統ID
CKOCMP-432508-Cpsf6-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Cpsf6-flox Mouse（カタログ番号S-CKO-11219）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Cpsf6-flox
系統ID
CKOCMP-432508-Cpsf6-B6N-VA
遺伝子名
製品ID
S-CKO-11219
遺伝子別名
CFIM, CFIM68, HPBRII-4, HPBRII-7, 4733401N12Rik
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conditional knockout
染色体
Chr 10
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000069168
NCBIトランスクリプトID
NM_001013391
ターゲット領域
Exon 4
有効領域の大きさ
~0.6 kb
遺伝子研究の概要
Cpsf6, also known as Cleavage and polyadenylation specificity factor subunit 6 or CFIm68, is a 68 kDa component of the mammalian cleavage factor I (CFIm) complex. It modulates mRNA alternative polyadenylation (APA), determining 3' untranslated region (UTR) length, an important gene expression control mechanism. Cpsf6 is also involved in the HIV-1 replication cycle, as it directly interacts with the HIV-1 core during infection [4].
In cancer cells, elevated Cpsf6 can lead to a systematic prolongation of 3' UTRs, yet its expression is typically higher in tumors than in healthy tissues. Cpsf6 can undergo liquid-liquid phase separation (LLPS), and elevated LLPS is associated with the preferential usage of the distal poly(A) sites. In lung adenocarcinoma, Cpsf6 elevation is attributed to BRCA1-disrupted R-loop accumulation in its 5' end, which leads to XBP1 3'UTR shortening, attenuating cisplatin-induced ER stress and elevating chemo-resistance. Inhibition of Cpsf6 expression in lung adenocarcinoma cell lines caused a significant reduction in cell proliferation, colony formation, and induced apoptosis. In hepatocellular carcinoma, Cpsf6 is upregulated and promotes tumorigenic activities. Depletion of Cpsf6 suppresses cell viability, colony formation, and the Warburg effect, and inhibits angiogenesis [1,2,3,6,7].
In the context of HIV-1 infection, preventing the formation of Cpsf6 condensates inhibits the infection of wild-type HIV-1. Also, cytoplasmic Cpsf6 regulates HIV-1 capsid trafficking and infection in a cyclophilin A-dependent manner [5,8].
In conclusion, Cpsf6 plays crucial roles in both cancer-related processes such as cell proliferation, apoptosis, chemo-resistance, and metabolism adaption, and in the HIV-1 replication cycle, including viral capsid trafficking and the formation of biomolecular condensates important for infection. Research on Cpsf6, especially through loss-of-function experiments, provides valuable insights into these disease areas, potentially guiding the development of new therapeutic strategies.
References：
1. Liu, Susu, Wu, Runze, Chen, Liutao, Fu, Yonggui, Xu, Anlong. 2023. CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation. In Cell reports, 42, 113197. doi:10.1016/j.celrep.2023.113197. https://pubmed.ncbi.nlm.nih.gov/37777964/
2. Zhu, Chuandong, Xie, Yufeng, Li, Qiang, Yu, Zhengyuan, Chen, Jing. 2023. CPSF6-mediated XBP1 3'UTR shortening attenuates cisplatin-induced ER stress and elevates chemo-resistance in lung adenocarcinoma. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 68, 100933. doi:10.1016/j.drup.2023.100933. https://pubmed.ncbi.nlm.nih.gov/36821972/
3. Zu, Yukun, Wang, Dao, Ping, Wei, Sun, Wei. 2022. The roles of CPSF6 in proliferation, apoptosis and tumorigenicity of lung adenocarcinoma. In Aging, 14, 9300-9316. doi:10.18632/aging.204407. https://pubmed.ncbi.nlm.nih.gov/36446361/
4. Bialas, Katarzyna, Diaz-Griffero, Felipe. 2024. HIV-1-induced translocation of CPSF6 to biomolecular condensates. In Trends in microbiology, 32, 781-790. doi:10.1016/j.tim.2024.01.001. https://pubmed.ncbi.nlm.nih.gov/38267295/
5. Luchsinger, Charlotte, Lee, KyeongEun, Mardones, Gonzalo A, KewalRamani, Vineet N, Diaz-Griffero, Felipe. 2023. Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection. In Scientific reports, 13, 10974. doi:10.1038/s41598-023-37364-x. https://pubmed.ncbi.nlm.nih.gov/37414787/
6. Tan, Sheng, Zhang, Ming, Shi, Xinglong, Sun, Jielin, Zhao, Xiaodong. 2021. CPSF6 links alternative polyadenylation to metabolism adaption in hepatocellular carcinoma progression. In Journal of experimental & clinical cancer research : CR, 40, 85. doi:10.1186/s13046-021-01884-z. https://pubmed.ncbi.nlm.nih.gov/33648552/
7. Sim, Deok Yong, Lee, Hyo-Jung, Ahn, Chi-Hoon, Kim, Bonglee, Kim, Sung-Hoon. 2024. Negative Regulation of CPSF6 Suppresses the Warburg Effect and Angiogenesis Leading to Tumor Progression Via c-Myc Signaling Network: Potential Therapeutic Target for Liver Cancer Therapy. In International journal of biological sciences, 20, 3442-3460. doi:10.7150/ijbs.93462. https://pubmed.ncbi.nlm.nih.gov/38993554/
8. Zhong, Zhou, Ning, Jiying, Boggs, Emerson A, Watkins, Simon C, Ambrose, Zandrea. 2021. Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner. In mBio, 12, . doi:10.1128/mBio.03142-20. https://pubmed.ncbi.nlm.nih.gov/33758083/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
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