Rgs1-flox Mouse
一般名
Rgs1-flox
製品ID
S-CKO-11391
背景情報
C57BL/6JCya
系統ID
CKOCMP-50778-Rgs1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Rgs1-flox Mouse（カタログ番号S-CKO-11391）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Rgs1-flox
系統ID
CKOCMP-50778-Rgs1-B6J-VA
遺伝子名
製品ID
S-CKO-11391
遺伝子別名
BL34
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 1
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000189061
NCBIトランスクリプトID
NM_015811
ターゲット領域
Exon 4
有効領域の大きさ
~1.3 kb
遺伝子研究の概要
Rgs1, short for Regulator of G-protein signaling 1, is a gene involved in multiple biological processes. It plays a role in regulating G-protein-coupled receptor (GPCR) signaling, which is crucial for various cellular functions such as cell communication, signal transduction, and cell response to stimuli [7]. Dysregulation of Rgs1 has implications in numerous disease conditions.
In alveolar macrophages, Rgs1 coregulates the immunophenotype of subpopulations through the PLC-IP3R signal-dependent intracellular Ca2+ response. Epithelium-and endothelium-derived exosomes target RGS1 expression, and imbalance of alveolar macrophages due to RGS1 dysregulation is linked to acute lung injury/acute respiratory distress syndrome (ALI/ARDS) and pulmonary fibrosis (PF) [1].
In renal interstitial fibrosis (RIF), RGS1 expression is elevated, and knockdown of RGS1 inhibits renal cell inflammatory response, fibrosis, and renal fibrosis in RIF mice, suggesting it mediates RIF through activation of the inflammatory response [2].
In cervical cancer, RGS1 is associated with the immune microenvironment and cell function. Knockdown of RGS1 in cervical cancer cell lines inhibits cell proliferation, migration, invasion, and tumor growth in nude mice and promotes apoptosis, indicating it may be a target for immunotherapy [3].
In gastric cancer, silencing the RGS1 gene reduces the proliferation of drug-resistant cells in vitro and inhibits tumor growth in vivo, making it an antitumor target [4].
In osteosarcoma, RGS1 is a tumor-promoting gene, and microRNA-376b-3p can target RGS1 to inhibit cell proliferation, metastasis, and apoptosis [6].
In spinal cord injury, inhibiting RGS1 attenuates secondary inflammation response and tissue degradation via the TLR/TRIF/NF-κB pathway in macrophages [5].
In summary, Rgs1 is involved in regulating immune responses, inflammation, and cell functions in various tissues. The use of gene knockdown and mouse models in these studies has revealed its significance in diseases like ALI/ARDS, PF, RIF, cervical cancer, gastric cancer, osteosarcoma, and spinal cord injury, highlighting its potential as a therapeutic target in these disease areas.
References：
1. Feng, Zunyong, Zhou, Jing, Liu, Yinhua, Wang, Yongsheng, Xia, Hongping. 2021. Epithelium- and endothelium-derived exosomes regulate the alveolar macrophages by targeting RGS1 mediated calcium signaling-dependent immune response. In Cell death and differentiation, 28, 2238-2256. doi:10.1038/s41418-021-00750-x. https://pubmed.ncbi.nlm.nih.gov/33753901/
2. Lu, Tefei, Chen, Sheng, Xu, Jianting. 2023. RGS1 mediates renal interstitial fibrosis through activation of the inflammatory response. In Archives of biochemistry and biophysics, 750, 109744. doi:10.1016/j.abb.2023.109744. https://pubmed.ncbi.nlm.nih.gov/37696381/
3. Zhang, Siyang, Wang, Han, Liu, Jiao, Zeng, Zhi, Wang, Min. 2022. RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation. In Journal of translational medicine, 20, 334. doi:10.1186/s12967-022-03526-0. https://pubmed.ncbi.nlm.nih.gov/35879796/
4. Chen, Zhixiong, Liu, Banglun, Zhang, Shouru, Lv, Yuyu, Sun, Hao. 2022. RGS1 serves as an antitumor target to inhibit proliferation of NICN87-DR cells and tumor growth in the gastric cancer mouse model. In Turkish journal of biology = Turk biyoloji dergisi, 46, 277-287. doi:10.55730/1300-0152.2616. https://pubmed.ncbi.nlm.nih.gov/37529094/
5. Feng, Dongqian, Yu, Jiasheng, Bao, Lei, Fan, Daobo, Zhang, Bin. 2021. Inhibiting RGS1 attenuates secondary inflammation response and tissue degradation via the TLR/TRIF/NF-κB pathway in macrophage post spinal cord injury. In Neuroscience letters, 768, 136374. doi:10.1016/j.neulet.2021.136374. https://pubmed.ncbi.nlm.nih.gov/34852285/
6. Zhang, Lei, Yao, Meng, Ma, Weikang, Jiang, Yongqing, Wang, Wenbo. . MicroRNA-376b-3p targets RGS1 mRNA to inhibit proliferation, metastasis, and apoptosis in osteosarcoma. In Annals of translational medicine, 9, 1652. doi:10.21037/atm-21-4949. https://pubmed.ncbi.nlm.nih.gov/34988161/
7. Liu, Tiezhu, Huang, Tao, Li, Jiajia, Wang, Shiwen, Liang, Mifang. 2023. Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA. In PloS one, 18, e0286056. doi:10.1371/journal.pone.0286056. https://pubmed.ncbi.nlm.nih.gov/37459313/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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