Ube2k-flox Mouse
一般名
Ube2k-flox
製品ID
S-CKO-11586
背景情報
C57BL/6JCya
系統ID
CKOCMP-53323-Ube2k-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Ube2k-flox Mouse（カタログ番号S-CKO-11586）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Ube2k-flox
系統ID
CKOCMP-53323-Ube2k-B6J-VA
遺伝子名
製品ID
S-CKO-11586
遺伝子別名
D5Ertd601e, E2-25k, HIP-2, Hip2, Hypg, Lig
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 5
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000142407
NCBIトランスクリプトID
NM_016786
ターゲット領域
Exon 4
有効領域の大きさ
~1.3 kb
遺伝子研究の概要
Ube2k, a ubiquitin-conjugating enzyme, is involved in the ubiquitin-proteasome system, responsible for numerous cellular physiological activities. It participates in the synthesis of K48-linked ubiquitin chains, which target substrates for proteasomal degradation [5]. This process is crucial for maintaining normal cellular function, regulating protein levels, and controlling various biological pathways.
In the context of diseases, in hepatocellular carcinoma (HCC), Ube2k expression is elevated, promoting cell proliferation, migration, and stemness property, and a worse prognosis for patients. Hypoxia can induce HIF-1α-mediated increase of Ube2k levels in HCC cells, and Ube2k cooperates with HIF-1α to form a functional axis to drive HCC progression [2,3].
In pancreatic ductal adenocarcinoma (PDAC), Ube2k is highly expressed, promoting cell proliferation and the stemness phenotype both in vitro and in vivo, and IGF2BP3 increases Ube2k expression by enhancing its RNA stability [4].
In glioma cells, Ube2k is highly expressed, and silencing it inhibits cell growth by inducing autophagy-related apoptosis [8].
In multiple myeloma, ixazomib, a 20S proteasome inhibitor, can inhibit cell proliferation by targeting Ube2k [7].
In depression, microglial circ-UBE2K binds to HNRNPU to upregulate Ube2k expression, leading to abnormal microglial activation, neuroinflammation, and promoting the development of major depressive disorder (MDD) [1].
In Alzheimer's disease, elevated Ube2k induced synaptic dysfunction through ubiquitinating K1082 on GluN2B [6].
In conclusion, Ube2k plays a significant role in multiple biological processes and disease conditions. Its involvement in cancer progression, neurodegenerative diseases like Alzheimer's, and mental disorders such as depression indicates its potential as a therapeutic target. Functional studies, including those using gene knockout or conditional knockout mouse models (if applicable in the future), will further enhance our understanding of Ube2k's role in these diseases, providing new strategies for diagnosis and treatment.
References：
1. Cai, Yujie, Ji, Yao, Liu, Yingxuan, Wang, Yan, Cui, Lili. 2024. Microglial circ-UBE2K exacerbates depression by regulating parental gene UBE2K via targeting HNRNPU. In Theranostics, 14, 4058-4075. doi:10.7150/thno.96890. https://pubmed.ncbi.nlm.nih.gov/38994030/
2. Lei, Xiangxiang, Hu, Xiaoge, Lu, Qiliang, Huang, Dongsheng, Xu, Qiuran. 2022. UBE2K promotes the malignant progression of hepatocellular carcinoma by regulating c-Myc. In Biochemical and biophysical research communications, 638, 210-218. doi:10.1016/j.bbrc.2022.11.046. https://pubmed.ncbi.nlm.nih.gov/36481361/
3. Ouyang, Guangxiong, Fu, Wen, Guo, Jinhui, Huang, Dongsheng, Zhang, Yaping. 2023. Hypoxia-induced UBE2K promotes the malignant progression of HCC. In Pathology, research and practice, 245, 154422. doi:10.1016/j.prp.2023.154422. https://pubmed.ncbi.nlm.nih.gov/37003132/
4. Fu, Wen, Lei, Xiangxiang, Lu, Qiliang, Tong, Xiangmin, Hu, Xiaoge. 2023. UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma. In International journal of oncology, 62, . doi:10.3892/ijo.2023.5500. https://pubmed.ncbi.nlm.nih.gov/36896783/
5. Nakasone, Mark A, Majorek, Karolina A, Gabrielsen, Mads, Smith, Brian O, Huang, Danny T. 2022. Structure of UBE2K-Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension. In Nature chemical biology, 18, 422-431. doi:10.1038/s41589-021-00952-x. https://pubmed.ncbi.nlm.nih.gov/35027744/
6. Wang, Xiong, Xie, Jiazhao, Tan, Lu, Li, Xiaoguang, Cheng, Liming. 2023. N6-methyladenosine-modified circRIMS2 mediates synaptic and memory impairments by activating GluN2B ubiquitination in Alzheimer's disease. In Translational neurodegeneration, 12, 53. doi:10.1186/s40035-023-00386-6. https://pubmed.ncbi.nlm.nih.gov/38012808/
7. Wang, Qingqing, Dong, Zhigao, Su, Junnan, Ma, Lili, Chen, Xuyan. 2021. Ixazomib inhibits myeloma cell proliferation by targeting UBE2K. In Biochemical and biophysical research communications, 549, 1-7. doi:10.1016/j.bbrc.2021.02.048. https://pubmed.ncbi.nlm.nih.gov/33647537/
8. Xin, Zhen, Holgersson, Kristian, Zhu, Pengcheng, Szekely, Laszlo, Wu, Tao. . Silencing UBE2K inhibits the growth of glioma cells by inducing the autophagy-related apoptosis. In Journal of biochemical and molecular toxicology, 38, e23758. doi:10.1002/jbt.23758. https://pubmed.ncbi.nlm.nih.gov/38963134/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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