Tgfbrap1-flox Mouse
一般名
Tgfbrap1-flox
製品ID
S-CKO-15631
背景情報
C57BL/6JCya
系統ID
CKOCMP-73122-Tgfbrap1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Tgfbrap1-flox Mouse（カタログ番号S-CKO-15631）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Tgfbrap1-flox
系統ID
CKOCMP-73122-Tgfbrap1-B6J-VA
遺伝子名
製品ID
S-CKO-15631
遺伝子別名
Trap1, 3110018K12Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 1
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000095014
NCBIトランスクリプトID
NM_001013025
ターゲット領域
Exon 5
有効領域の大きさ
~1.2 kb
遺伝子研究の概要
Tgfbrap1, also known as TGF-β receptor-associated binding protein 1, plays crucial roles in multiple biological processes. It is involved in the TGF-β signaling pathway, which regulates various cellular functions such as cell growth, differentiation, apoptosis, and extracellular matrix production. Dysregulation of this pathway can lead to numerous diseases [1,5,7].
In pulmonary fibrosis, Chitinase 1 (CHIT1) enhances TGF-β1-stimulated fibrotic responses through inhibiting TGF-β1 induction of SMAD7. This effect is mediated by CHIT1's interaction with Tgfbrap1, highlighting Tgfbrap1's role in the pathogenesis of pulmonary fibrosis [1,7].
In goose follicular granulosa cells, knockdown of Tgfbrap1 mRNA level reduces apoptosis rate and enhances the secretion of E2 and P4, indicating its role in regulating follicular development [2].
Also, a variant at Tgfbrap1 (rs2241797) is significantly associated with type 2 diabetes mellitus and affects diabetes-related miRNA expression [3].
Additionally, the A variant of rs17687727 in Tgfbrap1 influences the susceptibility to antituberculosis drug-induced liver injury in the Han Chinese population [4].
In liver cancer, Tgfbrap1 stabilizes TGF-β receptor type 1 (TGFBR1), potentiating TGF-β signaling and promoting liver cancer stemness and drug resistance [5].
Piperine, which protects against acetaminophen-induced hepatotoxicity, may mediate its effects through regulating Tgfbrap1 [6].
Overexpression of Tgfbrap1, a CORVET-specific subunit, decreases the amount of assembled HOPS complex, affecting autophagosome-lysosome fusion and lysosomal hydrolase delivery [8].
In conclusion, Tgfbrap1 is essential in the TGF-β signaling pathway and is involved in various biological processes and disease conditions such as pulmonary fibrosis, follicular development, type 2 diabetes, antituberculosis drug-induced liver injury, liver cancer, hepatotoxicity response, and endosomal tethering complex regulation. The study of Tgfbrap1 through genetic models helps to understand its function in these diseases, potentially providing new insights for disease treatment and prevention.
References：
1. Lee, Chang-Min, He, Chuan-Hua, Park, Jin Wook, Elias, Jack A, Lee, Chun Geun. 2024. Retraction: Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. In Life science alliance, 7, . doi:10.26508/lsa.202402987. https://pubmed.ncbi.nlm.nih.gov/39209538/
2. Wang, Zhixiu, Lu, Lu, Gu, Tiantian, Xu, Qi, Chen, Guohong. 2020. The effects of FAR1 and TGFBRAP1 on the proliferation and apoptosis of follicular granulosa cells in goose (Anser cygnoides). In Gene, 769, 145194. doi:10.1016/j.gene.2020.145194. https://pubmed.ncbi.nlm.nih.gov/33007376/
3. Yang, Song, Chen, Xiaotian, Yang, Mengyao, Liu, Chunlan, Shen, Chong. 2018. The variant at TGFBRAP1 is significantly associated with type 2 diabetes mellitus and affects diabetes-related miRNA expression. In Journal of cellular and molecular medicine, 23, 83-92. doi:10.1111/jcmm.13885. https://pubmed.ncbi.nlm.nih.gov/30461200/
4. Zhang, Jingwei, Zhao, Zhenzhen, Bai, Hao, Lyv, Mengyuan, Ying, Binwu. 2019. The Variant at TGFBRAP1 but Not TGFBR2 Is Associated with Antituberculosis Drug-Induced Liver Injury. In Evidence-based complementary and alternative medicine : eCAM, 2019, 1685128. doi:10.1155/2019/1685128. https://pubmed.ncbi.nlm.nih.gov/31534460/
5. Liu, Kewei, Tian, Fanxuan, Chen, Xu, Wang, Tao, Wang, Bin. 2024. Stabilization of TGF-β Receptor 1 by a Receptor-Associated Adaptor Dictates Feedback Activation of the TGF-β Signaling Pathway to Maintain Liver Cancer Stemness and Drug Resistance. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2402327. doi:10.1002/advs.202402327. https://pubmed.ncbi.nlm.nih.gov/38981014/
6. Morsy, M A, Younis, N S, El-Sheikh, A A K, El-Daly, M, Mohafez, O M. . Protective mechanisms of piperine against acetaminophen-induced hepatotoxicity may be mediated through TGFBRAP1. In European review for medical and pharmacological sciences, 24, 10169-10180. doi:10.26355/eurrev_202010_23237. https://pubmed.ncbi.nlm.nih.gov/33090425/
7. Lee, Chang-Min, He, Chuan-Hua, Park, Jin Wook, Elias, Jack A, Lee, Chun Geun. 2019. Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. In Life science alliance, 2, . doi:10.26508/lsa.201900350. https://pubmed.ncbi.nlm.nih.gov/31085559/
8. Sőth, Ármin, Molnár, Márton, Lőrincz, Péter, Simon-Vecsei, Zsófia, Juhász, Gábor. 2024. CORVET-specific subunit levels determine the balance between HOPS/CORVET endosomal tethering complexes. In Scientific reports, 14, 10146. doi:10.1038/s41598-024-59775-0. https://pubmed.ncbi.nlm.nih.gov/38698024/
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凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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