Snx27-flox Mouse
一般名
Snx27-flox
製品ID
S-CKO-16543
背景情報
C57BL/6JCya
系統ID
CKOCMP-76742-Snx27-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Snx27-flox Mouse（カタログ番号S-CKO-16543）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Snx27-flox
系統ID
CKOCMP-76742-Snx27-B6J-VA
遺伝子名
製品ID
S-CKO-16543
遺伝子別名
ESTM45, ESTM47, 5730552M22Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 3
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000107283
NCBIトランスクリプトID
NM_001082484
ターゲット領域
Exon 2
有効領域の大きさ
~1.1 kb
遺伝子研究の概要
Snx27 belongs to the sorting nexin (SNX) family of proteins. It is unique in containing both a Phox (PX) domain and an additional PDZ domain. Snx27 plays a critical role in protein sorting and trafficking in the endocytosis pathway, mediating the recycling of internalized proteins from endosomes to the plasma membrane in a PDZ domain-dependent manner. It is associated with the retromer complex and is involved in multiple cellular functions, which are essential for normal cellular function and preventing human diseases [1,3].
In T-cell activation, Snx27 translocates to the T cell-APC interface upon TCR activation, facilitating the transport of cargoes and interacting with DGKζ to sustain DAG modulation for IS organization and signaling [2]. In viral infections, Snx27 together with the retromer complex prevents the ACE2/virus complex from entering lysosome/late endosome, decreasing SARS-CoV-2 viral entry [4]. In cytomegalovirus replication, Snx27:Retromer:ESCPE-1-mediated tubulation is essential for the establishment of a Rab10-decorated subset of membranes within the pre-AC, and suppression of Snx27-mediated functions leads to a decrease in infectious virion release [7]. In neurological function, Snx27-mediated recycling of neuroligin-2 regulates inhibitory signaling by enhancing synaptic neuroligin-2 clusters [8]. In cancer, while its role in tumorigenesis is not fully understood, its over-expression is linked to tumorigenesis, cancer progression, and metastasis. For example, circ_Snx27 in hepatocellular carcinoma promotes cancer development via the miR-637/FGFR1 axis and circ-Snx27 sponging miR-375/RPN1 axis contributes to HCC progression [5,6]. Also, Snx27 inhibits TNFα-induced NF-κB signalling activation by facilitating OTULIN to localize to TNF receptor complex [9].
In conclusion, Snx27 is crucial for endosomal protein recycling and has diverse functions in various biological processes and diseases. Its role in T-cell activation, viral infections, cytomegalovirus replication, neurological function, and cancer development has been revealed through different studies. Understanding Snx27's functions provides insights into normal cellular function and offers potential strategies for treating related diseases.
References：
1. Deb, Shreya, Sun, Jun. 2022. Endosomal Sorting Protein SNX27 and Its Emerging Roles in Human Cancers. In Cancers, 15, . doi:10.3390/cancers15010070. https://pubmed.ncbi.nlm.nih.gov/36612066/
2. González-Mancha, Natalia, Mérida, Isabel. 2020. Interplay Between SNX27 and DAG Metabolism in the Control of Trafficking and Signaling at the IS. In International journal of molecular sciences, 21, . doi:10.3390/ijms21124254. https://pubmed.ncbi.nlm.nih.gov/32549284/
3. Chandra, Mintu, Kendall, Amy K, Jackson, Lauren P. 2021. Toward Understanding the Molecular Role of SNX27/Retromer in Human Health and Disease. In Frontiers in cell and developmental biology, 9, 642378. doi:10.3389/fcell.2021.642378. https://pubmed.ncbi.nlm.nih.gov/33937239/
4. Yang, Bo, Jia, Yuanyuan, Meng, Yumin, Qi, Jianxun, Shang, Guijun. . SNX27 suppresses SARS-CoV-2 infection by inhibiting viral lysosome/late endosome entry. In Proceedings of the National Academy of Sciences of the United States of America, 119, . doi:10.1073/pnas.2117576119. https://pubmed.ncbi.nlm.nih.gov/35022217/
5. Li, Hua, Liu, Bingli, Xu, Xin, Zhang, Di, Liu, Qingfeng. 2022. Circ_SNX27 regulates hepatocellular carcinoma development via miR-637/FGFR1 axis. In Environmental toxicology, 37, 2832-2843. doi:10.1002/tox.23640. https://pubmed.ncbi.nlm.nih.gov/36029209/
6. Zheng, Chao, Liang, Jin, Yu, Shoude, Dai, Lin, Xu, Dan. . Circ-SNX27 sponging miR-375/RPN1 axis contributes to hepatocellular carcinoma progression. In The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 27, 333-344. doi:10.4196/kjpp.2023.27.4.333. https://pubmed.ncbi.nlm.nih.gov/37386831/
7. Štimac, Igor, Marcelić, Marina, Radić, Barbara, Lučin, Pero, Mahmutefendić Lučin, Hana. 2024. SNX27:Retromer:ESCPE-1-mediated early endosomal tubulation impacts cytomegalovirus replication. In Frontiers in cellular and infection microbiology, 14, 1399761. doi:10.3389/fcimb.2024.1399761. https://pubmed.ncbi.nlm.nih.gov/39359939/
8. Halff, Els F, Szulc, Blanka R, Lesept, Flavie, Kittler, Josef T. . SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. In Cell reports, 29, 2599-2607.e6. doi:10.1016/j.celrep.2019.10.096. https://pubmed.ncbi.nlm.nih.gov/31775031/
9. Shi, Ruona, Shi, Xue, Qin, Dajiang, Vermeulen, Michiel, Zhang, Xiaofei. 2021. SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-κB signalling activation. In Cell & bioscience, 11, 146. doi:10.1186/s13578-021-00659-5. https://pubmed.ncbi.nlm.nih.gov/34315543/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。