Vsig1-flox Mouse
一般名
Vsig1-flox
製品ID
S-CKO-16865
背景情報
C57BL/6JCya
系統ID
CKOCMP-78789-Vsig1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Vsig1-flox Mouse（カタログ番号S-CKO-16865）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Vsig1-flox
系統ID
CKOCMP-78789-Vsig1-B6J-VA
遺伝子名
製品ID
S-CKO-16865
遺伝子別名
1700062D20Rik, 4930405J24Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr X
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000033806
NCBIトランスクリプトID
NM_030181
ターゲット領域
Exon 2
有効領域の大きさ
~1.0 kb
遺伝子研究の概要
Vsig1, a cell-cell adhesion molecule, is a member of the immunoglobulin superfamily. It is preferentially expressed in the stomach, testis, and certain cancers such as gastric, esophageal, and ovarian cancers [4]. In the stomach, it is involved in the proper differentiation of glandular gastric epithelia, being restricted to the adherens junction of the glandular epithelium [4]. In testis, it is located on the testicular germ cell surface and its extracellular domain interacts with Sertoli cell membrane protein while its PDZ-binding motif forms a complex with ZO-1 [7].
In gastric cancer, several studies have explored its role. Loss of VSIG1 expression is significantly correlated with poor overall and disease-free survival, and negative VSIG1 expression is related to tumor size, T, and M stage, suggesting it may be a prognostic marker and potential therapeutic target [5]. Also, its membrane-cytoplasmic translocation can indicate epithelial-mesenchymal transition due to the activation of the canonical Wnt/β-catenin signaling pathway [1]. In hepatocellular carcinoma, VSIG1 may be involved in the lineage shift to gastric-type HCC, and the E-cadherin/VSIG1 complex may help suppress tumor growth [6]. In colon cancer, VSIG1 restricts the development of pro-metastatic states, in part by inhibiting YAP/TAZ-TEAD signaling [2]. A study on VSIG1 knockout (KO) mice found that VSIG1 may not play essential roles in spermatogenesis and normal testis development, function, and maintenance in mice [3].
In conclusion, Vsig1 has important functions in the proper differentiation of glandular gastric epithelia and in testis cell interactions. In cancer, its expression and translocation are associated with disease progression in gastric and hepatocellular carcinomas, and it has an anti-metastatic role in colon cancer. The Vsig1 KO mouse model has been valuable in revealing its dispensability in spermatogenesis and male fertility in mice, contributing to our understanding of its role in specific biological processes and disease conditions.
References：
1. Satala, Catalin-Bogdan, Jung, Ioan, Kovacs, Zsolt, Patrichi, Andrei-Ionut, Gurzu, Simona. 2022. V-set and immunoglobulin domain containing 1 (VSIG1) as an emerging target for epithelial-mesenchymal transition of gastric cancer. In Scientific reports, 12, 16241. doi:10.1038/s41598-022-19883-1. https://pubmed.ncbi.nlm.nih.gov/36171238/
2. Bernal, Carolina, Silvano, Marianna, Tapponnier, Yann, Angulo, Cecilia, Ruiz i Altaba, Ariel. . Functional Pro-metastatic Heterogeneity Revealed by Spiked-scRNAseq Is Shaped by Cancer Cell Interactions and Restricted by VSIG1. In Cell reports, 33, 108372. doi:10.1016/j.celrep.2020.108372. https://pubmed.ncbi.nlm.nih.gov/33176137/
3. Jung, Yena, Bang, Hyewon, Kim, Young-Hyun, Kim, Sun-Uk, Kim, Ekyune. 2021. V-Set and Immunoglobulin Domain-Containing 1 (VSIG1), Predominantly Expressed in Testicular Germ Cells, Is Dispensable for Spermatogenesis and Male Fertility in Mice. In Animals : an open access journal from MDPI, 11, . doi:10.3390/ani11041037. https://pubmed.ncbi.nlm.nih.gov/33916888/
4. Oidovsambuu, Odgerel, Nyamsuren, Gunsmaa, Liu, Shuai, Engel, Wolfgang, Adham, Ibrahim M. 2011. Adhesion protein VSIG1 is required for the proper differentiation of glandular gastric epithelia. In PloS one, 6, e25908. doi:10.1371/journal.pone.0025908. https://pubmed.ncbi.nlm.nih.gov/21991385/
5. Chen, Yibing, Pan, Ke, Li, Shengping, He, Jia, Li, Qiao. 2011. Decreased expression of V-set and immunoglobulin domain containing 1 (VSIG1) is associated with poor prognosis in primary gastric cancer. In Journal of surgical oncology, 106, 286-93. doi:10.1002/jso.22150. https://pubmed.ncbi.nlm.nih.gov/22095633/
6. Gurzu, Simona, Sugimura, Haruhiko, Szederjesi, Janos, Fodor, Decebal, Jung, Ioan. 2021. Interaction between cadherins, vimentin, and V-set and immunoglobulin domain containing 1 in gastric-type hepatocellular carcinoma. In Histochemistry and cell biology, 156, 377-390. doi:10.1007/s00418-021-02006-8. https://pubmed.ncbi.nlm.nih.gov/34170400/
7. Kim, Ekyune, Lee, Youngjeon, Kim, Ji-Su, Hong, Yonggeun, Chang, Kyu-Tae. 2010. Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1. In Molecules and cells, 30, 443-8. doi:10.1007/s10059-010-0138-4. https://pubmed.ncbi.nlm.nih.gov/20957455/
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