Bmp4-flox Mouse
一般名
Bmp4-flox
製品ID
S-CKO-17613
背景情報
C57BL/6JCya
系統ID
CKOCMP-12159-Bmp4-B6J-VC
状況
このマウス系統を論文で使用する場合は、「Bmp4-flox Mouse（カタログ番号S-CKO-17613）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Bmp4-flox
系統ID
CKOCMP-12159-Bmp4-B6J-VC
遺伝子名
製品ID
S-CKO-17613
遺伝子別名
Bmp-4, Bmp2b, Bmp2b1, Bmp2b-1
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 14
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000074077
NCBIトランスクリプトID
NM_007554
ターゲット領域
Exon 3
有効領域の大きさ
~0.9 kb
遺伝子研究の概要
Bmp4, short for Bone Morphogenetic Protein 4, is a crucial protein in the BMP signaling pathway. This pathway is involved in multiple biological processes, including early embryonic morphogenesis, especially in the epiblast, as well as the development of extra-embryonic lineages such as trophectoderm [2,3].
In corneal neovascularization (CNV), Bmp4 inhibits the formation of tip cells by reducing the generation of neutrophil extracellular traps (NETs) through the NADPH oxidase-2 (NOX-2) pathway, disrupting the tip-stalk cell balance and thus inhibiting CNV, suggesting it as a potential therapeutic target [1]. In trophoblast development, BMP4-based protocols can convert pluripotent stem cells into trophoblast in vitro, and understanding its role helps model placental development [2,3]. Regarding non-syndromic cleft lip with or without cleft palate (NSCLP), a meta-analysis showed that BMP4 rs17563 polymorphism may be a risk factor in Chinese and Brazilian populations [4]. BMP4 also enhances cell migration and promotes epithelial-mesenchymal transition (EMT) through the Smad1 signaling pathway, which is associated with NSCLP [7]. In breast cancer, BMP4 suppresses metastasis by inhibiting cholesterol biosynthesis, and it may be a predictive biomarker for statin therapy benefit [5]. In endometrial stromal cells, BMP4 affects decidualization by regulating FOXO1 expression, and its down-regulation may contribute to recurrent spontaneous abortion (RSA) [6]. In glioblastoma, BMP4 inhibits invasion by promoting E-cadherin and claudin expression through SMAD signaling [8]. In human preimplantation embryos, BMP4 supplementation triggers apoptosis [9].
In conclusion, Bmp4 is essential in various biological processes such as embryonic development, cell differentiation, and apoptosis. Its role in diseases like CNV, NSCLP, breast cancer, RSA, and glioblastoma has been revealed through different research models. Understanding Bmp4 not only deepens our knowledge of normal biological functions but also provides potential therapeutic directions for these diseases.
References：
1. Nan, Weijin, He, Yuxi, Shen, Sitong, Wang, Shurong, Zhang, Yan. 2023. BMP4 inhibits corneal neovascularization by interfering with tip cells in angiogenesis. In Experimental eye research, 237, 109680. doi:10.1016/j.exer.2023.109680. https://pubmed.ncbi.nlm.nih.gov/37858608/
2. Roberts, R Michael, Ezashi, Toshihiko, Temple, Jasmine, Soncin, Francesca, Parast, Mana M. 2022. The role of BMP4 signaling in trophoblast emergence from pluripotency. In Cellular and molecular life sciences : CMLS, 79, 447. doi:10.1007/s00018-022-04478-w. https://pubmed.ncbi.nlm.nih.gov/35877048/
3. Li, Yingchun, Parast, Mana M. . BMP4 regulation of human trophoblast development. In The International journal of developmental biology, 58, 239-46. doi:10.1387/ijdb.130341mp. https://pubmed.ncbi.nlm.nih.gov/25023690/
4. Bahrami, Reza, Dastgheib, Seyed Alireza, Niktabar, Seyed Mohammadreza, Bahrololoomi, Zahra, Neamatzadeh, Hossein. 2020. Association of BMP4 rs17563 Polymorphism with Nonsyndromic Cleft Lip with or without Cleft Palate Risk: Literature Review and Comprehensive Meta-Analysis. In Fetal and pediatric pathology, 40, 305-319. doi:10.1080/15513815.2019.1707916. https://pubmed.ncbi.nlm.nih.gov/31909686/
5. Chi, Lap Hing, Redfern, Andrew D, Lim Kam Sian, Terry C C, Daly, Roger J, Anderson, Robin L. 2024. BMP4-Induced Suppression of Breast Cancer Metastasis Is Associated with Inhibition of Cholesterol Biosynthesis. In International journal of molecular sciences, 25, . doi:10.3390/ijms25179160. https://pubmed.ncbi.nlm.nih.gov/39273106/
6. Huang, Yanjie, Dai, Fangfang, Chen, Liping, Liu, Hua, Cheng, Yanxiang. . BMP4 in Human Endometrial Stromal Cells Can Affect Decidualization by Regulating FOXO1 Expression. In Endocrinology, 165, . doi:10.1210/endocr/bqae049. https://pubmed.ncbi.nlm.nih.gov/38679470/
7. Hong, Jia-Wei, Yu, Yue, Wang, Lu-Shan, Zhang, Mei-Rong, Xu, Li-Chun. 2022. BMP4 Regulates EMT to be Involved in non-Syndromic Cleft lip With or Without Palate. In The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association, 60, 1462-1473. doi:10.1177/10556656221105762. https://pubmed.ncbi.nlm.nih.gov/35702016/
8. Zhao, Xiangdong, Sun, Qian, Dou, Changwu, Chen, Qianxue, Liu, Baohui. 2019. BMP4 inhibits glioblastoma invasion by promoting E-cadherin and claudin expression. In Frontiers in bioscience (Landmark edition), 24, 1060-1070. doi:. https://pubmed.ncbi.nlm.nih.gov/30844730/
9. De Paepe, C, Aberkane, A, Dewandre, D, Tournaye, H, Van de Velde, H. 2018. BMP4 plays a role in apoptosis during human preimplantation development. In Molecular reproduction and development, 86, 53-62. doi:10.1002/mrd.23081. https://pubmed.ncbi.nlm.nih.gov/30372558/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
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