Ifitm1-flox Mouse
一般名
Ifitm1-flox
製品ID
S-CKO-17757
背景情報
C57BL/6JCya
系統ID
CKOCMP-68713-Ifitm1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Ifitm1-flox Mouse（カタログ番号S-CKO-17757）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Ifitm1-flox
系統ID
CKOCMP-68713-Ifitm1-B6J-VA
遺伝子名
製品ID
S-CKO-17757
遺伝子別名
Mil2, Mil-2, DSPA2a, 1110036C17Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 7
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000106042
NCBIトランスクリプトID
NM_026820
ターゲット領域
Exon 3
有効領域の大きさ
~1.3 kb
遺伝子研究の概要
Ifitm1, short for Interferon (IFN)-induced transmembrane protein 1, is a member of the IFN-induced transmembrane protein family. It plays a crucial role in the innate immune response triggered by viral infection, with functions including conferring cellular resistance to many viruses and being involved in intracellular cholesterol transport [2,6,5]. It is also associated with pathways like the STING-TBK1-IRF3 signaling pathway [3].
In cancer, overexpression of Ifitm1 promotes tumor cell proliferation, invasion, metastasis, angiogenesis, and therapeutic resistance in various tumor types such as gallbladder carcinoma, esophageal adenocarcinoma, colorectal cancer, and gastric cancer, making it an independent prognostic biomarker [1]. In colorectal cancer, Apc mutation induces Ifitm1 expression, and high Ifitm1 levels in cells reduce fibroblast extracellular vesicle (EV) uptake, with functional implications for cell proliferation [4]. In lung adenocarcinoma, high expression of Ifitm1, along with FOXM1 and PLK1, is inversely correlated with patient survival rate, as Ifitm1 enhances FoxM1-mediated signaling [3]. In brain metastasis from lung cancer, loss of Ifitm1 promotes brain colonization, while high Ifitm1 expression in incipient metastatic cells activates microglia and CD8+ T cells to eliminate cancer cells, and it also enhances the efficacy of PD-1 blockade [9].
In viral infections, overexpression of Ifitm1 suppresses porcine epidemic diarrhea virus (PEDV) proliferation [2]. It also enhances non-enveloped viral (Aichi virus) RNA replication by facilitating cholesterol transport to the Golgi [5]. In SARS-CoV-2 infection, IFITM1 methylation is higher in severe and critical patients, potentially suppressing the immune system and promoting viral replication [7], and IFITM1 inhibits Spike-mediated fusion of SARS-CoV-2-infected cells [8].
In conclusion, Ifitm1 has diverse functions in both antiviral immune responses and cancer-related processes. Model-based research, especially in the context of cancer and viral infections, has revealed its significance in promoting or suppressing disease progression, highlighting its potential as a therapeutic target in these disease areas.
References：
1. Liang, Renba, Li, Xinxiao, Zhu, Xiaodong. . Deciphering the Roles of IFITM1 in Tumors. In Molecular diagnosis & therapy, 24, 433-441. doi:10.1007/s40291-020-00469-4. https://pubmed.ncbi.nlm.nih.gov/32394410/
2. Cheng, Jiahao, He, Jiayi, Feng, Simeng, Wang, Aibing, Yuan, Xiaomin. 2024. IFITM1 is a host restriction factor that inhibits porcine epidemic diarrhea virus infection. In Journal of nanobiotechnology, 22, 677. doi:10.1186/s12951-024-02884-9. https://pubmed.ncbi.nlm.nih.gov/39501328/
3. Xu, Rong, Lee, Young-Joo, Kim, Chang-Hyeon, Kim, Jung-Hyun, Yim, Hyungshin. 2023. Invasive FoxM1 phosphorylated by PLK1 induces the polarization of tumor-associated macrophages to promote immune escape and metastasis, amplified by IFITM1. In Journal of experimental & clinical cancer research : CR, 42, 302. doi:10.1186/s13046-023-02872-1. https://pubmed.ncbi.nlm.nih.gov/37968723/
4. Kelemen, Andrea, Carmi, Idan, Oszvald, Ádám, Buzás, Edit I, Wiener, Zoltán. 2021. IFITM1 expression determines extracellular vesicle uptake in colorectal cancer. In Cellular and molecular life sciences : CMLS, 78, 7009-7024. doi:10.1007/s00018-021-03949-w. https://pubmed.ncbi.nlm.nih.gov/34609520/
5. Ishikawa-Sasaki, Kumiko, Murata, Takayuki, Sasaki, Jun. 2023. IFITM1 enhances nonenveloped viral RNA replication by facilitating cholesterol transport to the Golgi. In PLoS pathogens, 19, e1011383. doi:10.1371/journal.ppat.1011383. https://pubmed.ncbi.nlm.nih.gov/37252940/
6. Yánez, Diana C, Ross, Susan, Crompton, Tessa. 2019. The IFITM protein family in adaptive immunity. In Immunology, 159, 365-372. doi:10.1111/imm.13163. https://pubmed.ncbi.nlm.nih.gov/31792954/
7. Arefinia, Nasir, Yaghoubi, Ramin, Ramezani, Amin, Zadeh, Ali Mohammad Arab, Sarvari, Jamal. . Association of IFITM1 Promoter Methylation with Severity of SARS-CoV-2 Infection. In Clinical laboratory, 69, . doi:10.7754/Clin.Lab.2022.220622. https://pubmed.ncbi.nlm.nih.gov/37057950/
8. Buchrieser, Julian, Dufloo, Jérémy, Hubert, Mathieu, Bruel, Timothée, Schwartz, Olivier. 2020. Syncytia formation by SARS-CoV-2-infected cells. In The EMBO journal, 39, e106267. doi:10.15252/embj.2020106267. https://pubmed.ncbi.nlm.nih.gov/33051876/
9. She, Xiaofei, Shen, Shijun, Chen, Guang, Qin, Huanlong, Gao, Hua. 2023. Immune surveillance of brain metastatic cancer cells is mediated by IFITM1. In The EMBO journal, 42, e111112. doi:10.15252/embj.2022111112. https://pubmed.ncbi.nlm.nih.gov/36799040/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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