Crip1-flox Mouse
一般名
Crip1-flox
製品ID
S-CKO-18201
背景情報
C57BL/6JCya
系統ID
CKOCMP-12925-Crip1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Crip1-flox Mouse（カタログ番号S-CKO-18201）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Crip1-flox
系統ID
CKOCMP-12925-Crip1-B6J-VA
遺伝子名
製品ID
S-CKO-18201
遺伝子別名
CRHP, CRP1, Crip
遺伝子別名
C57BL/6JCya
遺伝子正式名称
cysteine-rich protein 1
NCBI ID
修正
Conditional knockout
染色体
Chr 12
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000006523
NCBIトランスクリプトID
NM_007763
ターゲット領域
Exon 2~5
有効領域の大きさ
~1.6 kb
遺伝子研究の概要
CRIP1, also known as cysteine-rich intestinal protein 1, encodes a member of the LIM/double zinc finger protein family. It is involved in regulating diverse cellular processes such as cell growth, development, and differentiation. CRIP1 is associated with multiple signaling pathways including NF-κB, Wnt/β-catenin, and is important in maintaining protein homeostasis [1,2,5,7]. Genetic models, especially KO/CKO mouse models, could potentially offer insights into its functions.
In pancreatic ductal adenocarcinoma (PDAC), CRIP1 upregulation fosters MDSC trafficking and an immunosuppressive tumor microenvironment by facilitating NF-κB/p65 nuclear translocation [1]. In hepatocellular carcinoma (HCC), it promotes cancer stem-like properties by suppressing BBOX1-mediated carnitine metabolism [2]. In gastric cancer, CRIP1 overexpression promotes lymphangiogenesis and lymphatic metastasis by phosphorylating CREB1 and regulating VEGFC and CCL5 expression [3]. In multiple myeloma, high CRIP1 expression is linked to unfavorable outcomes, and it dual-regulates proteasome and autophagy activities [4]. In osteoporosis, reduced CRIP1 expression in bone marrow stromal cells (BMSCs) impairs osteogenic differentiation, while overexpression enhances it via the Wnt signaling pathway [5]. In acute myeloid leukemia (AML), high CRIP1 expression is related to lower remission rates and shorter survival, and in AML-M5 subtype cells, it supports growth and migration by activating the Wnt/β-catenin pathway [6,7]. In gastric cancer, CRIP1 promotes homologous repair upon DNA damage from chemotherapy by cooperating with BRCA2 to enrich nuclear RAD51 [8].
In conclusion, CRIP1 is a crucial regulator in various biological processes and diseases. Through model-based research, its roles in tumorigenesis, immune regulation, bone metabolism, and leukemia development have been revealed. The use of KO/CKO mouse models could further clarify its functions in these disease areas, providing potential therapeutic targets for PDAC, HCC, gastric cancer, multiple myeloma, osteoporosis, and AML.
References：
1. Liu, Xiaomeng, Tang, Rong, Xu, Jin, Yu, Xianjun, Shi, Si. 2023. CRIP1 fosters MDSC trafficking and resets tumour microenvironment via facilitating NF-κB/p65 nuclear translocation in pancreatic ductal adenocarcinoma. In Gut, 72, 2329-2343. doi:10.1136/gutjnl-2022-329349. https://pubmed.ncbi.nlm.nih.gov/37541772/
2. Wang, Jing, Zhou, Yan, Zhang, Donghui, Wang, Hui, Zhao, Liang. 2022. CRIP1 suppresses BBOX1-mediated carnitine metabolism to promote stemness in hepatocellular carcinoma. In The EMBO journal, 41, e110218. doi:10.15252/embj.2021110218. https://pubmed.ncbi.nlm.nih.gov/35775648/
3. Wu, Zhonghua, Qu, Bicheng, Yuan, Minxian, Song, Yongxi, Wang, Zhenning. 2023. CRIP1 Reshapes the Gastric Cancer Microenvironment to Facilitate Development of Lymphatic Metastasis. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303246. doi:10.1002/advs.202303246. https://pubmed.ncbi.nlm.nih.gov/37409440/
4. Tang, Peixia, Yu, Zhen, Sun, Hao, Qiu, Lugui, Hao, Mu. 2024. CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy. In EBioMedicine, 100, 104961. doi:10.1016/j.ebiom.2023.104961. https://pubmed.ncbi.nlm.nih.gov/38199044/
5. Chen, Ruge, Jin, Yangchen, Lian, Ru, Zhang, Zhongmin, Zhao, Liang. 2024. CRIP1 regulates osteogenic differentiation of bone marrow stromal cells and pre-osteoblasts via the Wnt signaling pathway. In Biochemical and biophysical research communications, 727, 150277. doi:10.1016/j.bbrc.2024.150277. https://pubmed.ncbi.nlm.nih.gov/38936225/
6. Ma, Bei-Bei, Zhang, Ting-Juan, Wang, Cui-Zhu, Lin, Jiang, Qian, Jun. 2020. Methylation-independent CRIP1 expression is a potential biomarker affecting prognosis in cytogenetically normal acute myeloid leukemia. In American journal of translational research, 12, 4840-4852. doi:. https://pubmed.ncbi.nlm.nih.gov/33042393/
7. Deng, Xiaoling, Zeng, Yanmei, Qiu, Xiaofen, Zhang, Jingdong, Chen, Xiaoli. 2023. CRIP1 supports the growth and migration of AML-M5 subtype cells by activating Wnt/β-catenin pathway. In Leukemia research, 130, 107312. doi:10.1016/j.leukres.2023.107312. https://pubmed.ncbi.nlm.nih.gov/37224580/
8. Sun, Huiying, Zhou, Rui, Zheng, Yannan, Shi, Min, Liao, Wangjun. 2021. CRIP1 cooperates with BRCA2 to drive the nuclear enrichment of RAD51 and to facilitate homologous repair upon DNA damage induced by chemotherapy. In Oncogene, 40, 5342-5355. doi:10.1038/s41388-021-01932-0. https://pubmed.ncbi.nlm.nih.gov/34262130/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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