Cpne1-flox Mouse
一般名
Cpne1-flox
製品ID
S-CKO-18528
背景情報
C57BL/6JCya
系統ID
CKOCMP-266692-Cpne1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Cpne1-flox Mouse（カタログ番号S-CKO-18528）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Cpne1-flox
系統ID
CKOCMP-266692-Cpne1-B6J-VA
遺伝子名
製品ID
S-CKO-18528
遺伝子別名
mKIAA4108, 1810028N16Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conditional knockout
染色体
Chr 2
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000109608
NCBIトランスクリプトID
NM_170588
ターゲット領域
Exon 3~14
有効領域の大きさ
~3.8 kb
遺伝子研究の概要
Cpne1, a calcium-dependent, phospholipid-binding protein, is ubiquitously expressed in various tissues and organs. It participates in multiple biological processes, such as cell proliferation, apoptosis, differentiation, and is associated with pathways like MAPK, PERK-eIF2α, PI3K/AKT/HIF-1α, and MET signaling pathways [1,2,6,8]. Genetic models, especially KO mouse models, are valuable for studying its functions.
In gastric, pancreatic, non-small cell lung, breast, and hepatocellular carcinomas, Cpne1 silencing inhibits cell proliferation, induces apoptosis, and arrests the cell cycle. For example, in gastric cancer, Cpne1 knockdown inhibits tumor growth in vivo and in vitro by deactivating the DDIT3-FOS-MKNK2 axis of the MAPK pathway [1]. In pancreatic cancer, silencing the Cpne1-TRAF2 axis restrains cancer development [3]. In non-small cell lung cancer, Cpne1 promotes tumorigenesis by interacting with RACK1 and activating the MET signaling pathway [6]. In breast cancer, Cpne1 mediates glycolysis and metastasis through the PI3K/AKT/HIF-1α signaling pathway [8]. In hepatocellular carcinoma, knockdown of Cpne1 inhibits the AKT/P53 pathway, reducing cell proliferation, migration, and invasion [9]. In sarcopenia, Cpne1 overexpression in young muscles disrupts mitochondrial function and myogenesis via the PERK-eIF2α pathway [2]. In tooth germ development, Cpne1 is involved in odontoblastic differentiation of stem cells from apical papilla related to the AKT signaling pathway [7]. In neurons, HAX1 binds to Cpne1 to negatively affect Cpne1-mediated neuronal differentiation [4]. In sperm, Cpne1 deficiency preserves sperm motility under Ca2+ channel blockade [5].
In conclusion, Cpne1 plays crucial roles in cell-related biological processes and is significantly involved in multiple diseases, especially various cancers. The use of Cpne1 KO/CKO mouse models has revealed its role in promoting cancer progression and its association with disease-related pathways, providing potential therapeutic targets for these diseases.
References：
1. Li, Yan, Li, Lixiang, Liu, Han, Zhou, Tao. 2022. CPNE1 silencing inhibits cell proliferation and accelerates apoptosis in human gastric cancer. In European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 177, 106278. doi:10.1016/j.ejps.2022.106278. https://pubmed.ncbi.nlm.nih.gov/35985444/
2. Chen, Lin, Pan, Ling, Zeng, Yuexi, Zhu, Xiaonan, You, Li. 2022. CPNE1 regulates myogenesis through the PERK-eIF2α pathway mediated by endoplasmic reticulum stress. In Cell and tissue research, 391, 545-560. doi:10.1007/s00441-022-03720-y. https://pubmed.ncbi.nlm.nih.gov/36525128/
3. Song, Yelin, Song, Baolian, Yu, Zhichao, Lu, Zhongwen, Li, Zuowei. . Silencing of CPNE1-TRAF2 Axis Restrains the Development of Pancreatic Cancer. In Frontiers in bioscience (Landmark edition), 28, 316. doi:10.31083/j.fbl2811316. https://pubmed.ncbi.nlm.nih.gov/38062811/
4. Choi, Hye Young, Park, Nammi, Lee, Boah, Park, Jae-Yong, Yoo, Jae Cheal. 2020. CPNE1-mediated neuronal differentiation can be inhibited by HAX1 expression in HiB5 cells. In Biochemical and biophysical research communications, 533, 319-324. doi:10.1016/j.bbrc.2020.09.037. https://pubmed.ncbi.nlm.nih.gov/32958249/
5. Xie, Qiang, Zhang, Hanbin, Zhuang, Yuge, Chen, Zhenguo, Zhang, Guofei. 2024. Cpne1 deficiency preserves sperm motility under Ca2+ channel blockade. In The Journal of reproduction and development, 70, 309-319. doi:10.1262/jrd.2024-027. https://pubmed.ncbi.nlm.nih.gov/39010238/
6. Wang, Anqi, Yang, Wen, Li, Yue, Liu, Zeyi, Huang, Jian-An. 2022. CPNE1 promotes non-small cell lung cancer progression by interacting with RACK1 via the MET signaling pathway. In Cell communication and signaling : CCS, 20, 16. doi:10.1186/s12964-021-00818-8. https://pubmed.ncbi.nlm.nih.gov/35101055/
7. Lu, Xi, Xie, Han, Ju, Yanqin, Liu, Shangfeng, Zhao, Shouliang. 2023. Role of CPNE1 in Odontoblastic Differentiation of Rat Stem Cells from Apical Papilla. In Advanced biology, 7, e2300054. doi:10.1002/adbi.202300054. https://pubmed.ncbi.nlm.nih.gov/37132099/
8. Cao, Jingying, Cao, Renxian, Liu, Yiqi, Dai, Tao. 2023. CPNE1 mediates glycolysis and metastasis of breast cancer through activation of PI3K/AKT/HIF-1α signaling. In Pathology, research and practice, 248, 154634. doi:10.1016/j.prp.2023.154634. https://pubmed.ncbi.nlm.nih.gov/37454492/
9. Su, Jinfang, Huang, Yongbiao, Wang, Yali, Zhang, Hao, Xiong, Huihua. 2022. CPNE1 is a potential prognostic biomarker, associated with immune infiltrates and promotes progression of hepatocellular carcinoma. In Cancer cell international, 22, 67. doi:10.1186/s12935-022-02485-2. https://pubmed.ncbi.nlm.nih.gov/35139863/
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