Kntc1-flox Mouse

KNTC1, kinetochore associated 1, is a protein involved in chromosome segregation, being a component of the Rod-Zwilch-ZW10 (RZZ) complex essential for sister chromatid segregation during mitosis and participating in the spindle checkpoint [5]. It may also be associated with pathways like PI3K/Akt/mTOR [1]. Understanding its function is crucial for clarifying cell division-related biological processes.

In multiple cancers, loss-of-function studies have shown significant impacts. In gastric cancer, KNTC1 knockdown suppressed cell proliferation and migration while promoting apoptosis, potentially via the PI3K/Akt/mTOR pathway [1]. In liver cancer, it inhibited cell viability, migration, and invasion, arrested the cell cycle at the S-phase through CDK1 modulation, and decreased tumor growth in mouse transplanted tumors [2]. In pancreatic cancer, KNTC1 knockdown decreased cell growth and increased apoptosis [3]. In colon cancer, it reduced cell growth rates, increased apoptosis, and arrested cells in the S phase [4]. In non-small-cell lung cancer, it restrained cell viability, proliferation, migration, and invasion, and inhibited tumorigenesis in a xenograft model [6].

In conclusion, KNTC1 plays a significant role in cell division-related biological processes. Through gene knockout or knockdown models in various cancer types such as gastric, liver, pancreatic, colon, and non-small-cell lung cancers, it has been revealed that KNTC1 promotes cancer cell proliferation, migration, and tumor growth, making it a potential biomarker and therapeutic target for these cancers.