Cry2-KO Mouse
一般名
Cry2-KO
製品ID
S-KO-01638
背景情報
C57BL/6NCya
系統ID
KOCMP-12953-Cry2-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Cry2-KO Mouse（カタログ番号S-KO-01638）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Cry2-KO
系統ID
KOCMP-12953-Cry2-B6N-VA
遺伝子名
製品ID
S-KO-01638
遺伝子別名
D130054K12Rik
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conventional knockout
染色体
Chr 2
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000090559
NCBIトランスクリプトID
NM_009963
ターゲット領域
Exon 2~5
有効領域の大きさ
~4.8 kb
遺伝子研究の概要
Cry2, or Cryptochrome 2, is a gene encoding a protein that plays diverse and crucial roles. In plants, it serves as a blue-light photoreceptor regulating various growth aspects, and in mammals, it is a core circadian clock regulator. In the plant circadian clock, it represses CLOCK/BMAL1-induced transcription activation, while in mammals, it is related to DNA-repair enzymes and modulates transcriptional response to DNA damage. It is involved in pathways like photomorphogenesis in plants, and in mammals, it impacts processes related to cell growth, adipogenesis, and circadian-related functions [1,2,3,4,5,6,7].
In Arabidopsis, loss-of-function studies showed that CRY2 suppresses cell division in the root apical meristem to downregulate root elongation in darkness, and blue-light oligomerizes CRY2 to de-repress root elongation [1]. In adipogenesis, disruption of the interaction between Cry2 and Per2 through a C432 mutation in Cry2 led to loss of repression of clock transcription activation and suppressed adipocyte differentiation, indicating Cry2 promotes adipogenesis via this interaction [6,7]. In 5xFAD mice, down-regulation of CRY2 attenuated the sleep-deprivation-induced cognitive decline, suggesting CRY2 mediates this cognitive decline [8]. In recurrent spontaneous abortion, knockdown of CRY2 in HTR-8/SVneo cells promoted trophoblast migration and invasion, while overexpression had the opposite effect, as CRY2 inhibits the c-Myc-BMAL1-MMP2/9 pathway [9].
In conclusion, Cry2 is essential for regulating plant growth, adipogenesis, cognitive function, and trophoblast behavior. Studies using gene-knockout or mutation models in plants and mice have revealed its role in these biological processes. In the context of diseases, understanding Cry2 function may offer insights into treating conditions like cognitive decline and recurrent spontaneous abortion.
References:
1. Zeng, Desheng, Lv, Junqing, Li, Xu, Liu, Hongtao. 2024. The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth. In Cell, 188, 60-76.e20. doi:10.1016/j.cell.2024.10.031. https://pubmed.ncbi.nlm.nih.gov/39549699/
2. Jiang, Bochen, Zhong, Zhenhui, Gu, Lianfeng, Wang, Xu, Lin, Chentao. 2023. Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis. In Nature plants, 9, 2042-2058. doi:10.1038/s41477-023-01580-0. https://pubmed.ncbi.nlm.nih.gov/38066290/
3. Li, Youping, Shi, Yiting, Li, Minze, Liu, Hongtao, Yang, Shuhua. . The CRY2-COP1-HY5-BBX7/8 module regulates blue light-dependent cold acclimation in Arabidopsis. In The Plant cell, 33, 3555-3573. doi:10.1093/plcell/koab215. https://pubmed.ncbi.nlm.nih.gov/34427646/
4. Miller, Simon, Kesherwani, Manish, Chan, Priscilla, Kay, Steve A, Hirota, Tsuyoshi. 2022. CRY2 isoform selectivity of a circadian clock modulator with antiglioblastoma efficacy. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2203936119. doi:10.1073/pnas.2203936119. https://pubmed.ncbi.nlm.nih.gov/36161947/
5. Chan, Alanna B, Parico, Gian Carlo G, Fribourgh, Jennifer L, Partch, Carrie L, Lamia, Katja A. . CRY2 missense mutations suppress P53 and enhance cell growth. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2101416118. https://pubmed.ncbi.nlm.nih.gov/34183418/
6. Li, Weini, Xiong, Xuekai, Kiperman, Tali, Ma, Ke. 2023. Transcription repression of Cry2 via Per2 interaction promotes adipogenesis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.03.12.532323. https://pubmed.ncbi.nlm.nih.gov/36993226/
7. Li, Weini, Xiong, Xuekai, Kiperman, Tali, Ma, Ke. 2023. Transcription Repression of CRY2 via PER2 Interaction Promotes Adipogenesis. In Molecular and cellular biology, 43, 500-514. doi:10.1080/10985549.2023.2253710. https://pubmed.ncbi.nlm.nih.gov/37724597/
8. Luo, Sumei, Guo, Lizhe, Chen, Na, Wang, Yunjiao, Wang, E. 2024. CRY2 mediates the cognitive decline induced by sleep deprivation in 5xFAD mice. In PloS one, 19, e0306930. doi:10.1371/journal.pone.0306930. https://pubmed.ncbi.nlm.nih.gov/39012854/
9. Wu, Lianzhi, Cheng, Biheng, Liu, Qian, Jiang, Ping, Yang, Jing. . CRY2 suppresses trophoblast migration and invasion in recurrent spontaneous abortion. In Journal of biochemistry, 167, 79-87. doi:10.1093/jb/mvz076. https://pubmed.ncbi.nlm.nih.gov/31536114/
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