Asb13-KO Mouse

Asb13, an ankyrin repeat and suppressor of cytokine signaling (SOCS) box-containing E3 ubiquitin ligase, is evolutionarily conserved and may be involved in regulating compartment size, such as in the brain and muscle [2]. It is also implicated in canonical Notch signaling and mitochondrial function [2].

In breast cancer, ASB13 knockout in cells promoted cell migration and decreased F-actin polymerization, while its overexpression suppressed lung metastasis. ASB13 acts by promoting the ubiquitination and degradation of transcription factor SNAI2, which in turn relieves the transcriptional repression of YAP, a suppressor of breast cancer progression. Clinical data shows ASB13 expression is positively correlated with improved overall survival in breast cancer patients [1]. In young women with breast cancer, CNA mutations in ASB13 were associated with higher gene expression and a worse survival outcome [3]. In acute myocardial infarction treated with extracorporeal membrane oxygenation, ASB13 was identified as a prognostic marker, and its co-expressed proteins are related to post-translational protein modification, cullin-RING ubiquitin ligase complex, and ubiquitin-mediated proteolysis [4].

In summary, Asb13 plays significant roles in breast cancer metastasis and prognosis, as well as in the context of acute myocardial infarction treatment. Gene knockout-based research, like in breast cancer cells, has been crucial in revealing its role in suppressing breast cancer metastasis by modulating key proteins and pathways [1,3].

References:

1. Fan, Huijuan, Wang, Xuxiang, Li, Wenyang, Zheng, Hanqiu, Kang, Yibin. 2020. ASB13 inhibits breast cancer metastasis through promoting SNAI2 degradation and relieving its transcriptional repression of YAP. In Genes & development, 34, 1359-1372. doi:10.1101/gad.339796.120. https://pubmed.ncbi.nlm.nih.gov/32943576/

2. Liu, Pengyu, Verhaar, Auke P, Peppelenbosch, Maikel P. 2018. Signaling Size: Ankyrin and SOCS Box-Containing ASB E3 Ligases in Action. In Trends in biochemical sciences, 44, 64-74. doi:10.1016/j.tibs.2018.10.003. https://pubmed.ncbi.nlm.nih.gov/30446376/

3. Chi, Chen, Murphy, Leigh C, Hu, Pingzhao. 2018. Recurrent copy number alterations in young women with breast cancer. In Oncotarget, 9, 11541-11558. doi:10.18632/oncotarget.24336. https://pubmed.ncbi.nlm.nih.gov/29545918/

4. Yang, Jingqi, Ouyang, Xiaochao, Yang, Ming, Xie, Guobo, Cao, Qianqiang. 2022. Identification of key programmed cell death-related genes and immune infiltration in extracorporeal membrane oxygenation treatment for acute myocardial infarction based on bioinformatics analysis. In Frontiers in cardiovascular medicine, 9, 1018662. doi:10.3389/fcvm.2022.1018662. https://pubmed.ncbi.nlm.nih.gov/36531699/