Myo1f-KO Mouse

Myo1f, an unconventional long-tailed class Ⅰ myosin, plays significant roles in immune cell functions, including motility, adhesion, and migration, and is involved in multiple immune-related pathways [2,3,7]. It also contributes to antifungal immunity, T-cell activation, and metabolism, and is associated with diseases such as fungal infections, peripheral T-cell lymphoma (PTCL), and allergic reactions [1,4,5]. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.

Myo1f-deficient mice are more susceptible to systemic Candida albicans infections, indicating its role in promoting antifungal immunity by regulating α-tubulin acetylation [1]. In γδT intraepithelial lymphocytes, Myo1f is essential for adhesion and migration. Its absence affects lymphocyte homing due to reduced CCR9 and α4β7 surface expression [2]. In mast cells, knockdown of Myo1F reduces degranulation induced by IgE cross-linking and MRGPRX2 stimulation, suggesting its role in allergic and pseudoallergic drug reactions [3]. T-cell-specific Myo1f knockout mice exhibit an increased tumor burden and attenuated experimental autoimmune encephalomyelitis (EAE) severity due to impaired T-cell activation [4]. In Aspergillus fumigatus keratitis mouse models, knockdown of Myo1f reduces disease severity, decreases neutrophil and macrophage recruitment, and suppresses the MyD88/NF-κB signaling pathway [6].

In conclusion, Myo1f is crucial for immune cell-related functions and has implications in fungal infections, allergic responses, and PTCL. KO/CKO mouse models have been instrumental in revealing its role in these disease-related biological processes, providing insights into potential therapeutic strategies for these conditions.

References:

1. Sun, Wanwei, Ma, Xiaojian, Wang, Heping, Li, Xiaoxia, Wang, Chenhui. . MYO1F regulates antifungal immunity by regulating acetylation of microtubules. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2100230118. https://pubmed.ncbi.nlm.nih.gov/34301894/

2. Martínez-Vargas, Irving Ulises, Sánchez-Bello, Maria Elena, Miguel-Rodríguez, Carlos Emilio, Santos-Argumedo, Leopoldo, Talamás-Rohana, Patricia. 2023. Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration. In Frontiers in immunology, 14, 1041079. doi:10.3389/fimmu.2023.1041079. https://pubmed.ncbi.nlm.nih.gov/37207213/

3. Navinés-Ferrer, Arnau, Ainsua-Enrich, Erola, Serrano-Candelas, Eva, Olivera, Ana, Martin, Margarita. 2021. MYO1F Regulates IgE and MRGPRX2-Dependent Mast Cell Exocytosis. In Journal of immunology (Baltimore, Md. : 1950), 206, 2277-2289. doi:10.4049/jimmunol.2001211. https://pubmed.ncbi.nlm.nih.gov/33941653/

4. Cui, Zhihui, Wang, Heping, Feng, Xiong, Xiao, Xue, Wang, Chenhui. 2024. MYO1F regulates T-cell activation and glycolytic metabolism by promoting the acetylation of GAPDH. In Cellular & molecular immunology, 22, 176-190. doi:10.1038/s41423-024-01247-6. https://pubmed.ncbi.nlm.nih.gov/39668163/

5. Cortes, Jose R, Filip, Ioan, Albero, Robert, Rabadan, Raul, Palomero, Teresa. . Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma. In Cell reports, 39, 110695. doi:10.1016/j.celrep.2022.110695. https://pubmed.ncbi.nlm.nih.gov/35443168/

6. Liu, Wenting, Yang, Hua, Xu, Qiang, Sun, Xiaoyan, Che, Chengye. 2024. Role of MYO1F in neutrophil and macrophage recruitment and pro-inflammatory cytokine production in Aspergillus fumigatus keratitis. In International immunopharmacology, 142, 113094. doi:10.1016/j.intimp.2024.113094. https://pubmed.ncbi.nlm.nih.gov/39276460/

7. Navinés-Ferrer, Arnau, Ainsua-Enrich, Erola, Serrano-Candelas, Eva, Sayós, Joan, Martin, Margarita. 2019. Myo1f, an Unconventional Long-Tailed Myosin, Is a New Partner for the Adaptor 3BP2 Involved in Mast Cell Migration. In Frontiers in immunology, 10, 1058. doi:10.3389/fimmu.2019.01058. https://pubmed.ncbi.nlm.nih.gov/31143189/