Prpf4b-KO Mouse
一般名
Prpf4b-KO
製品ID
S-KO-03801
背景情報
C57BL/6JCya
系統ID
KOCMP-19134-Prpf4b-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Prpf4b-KO Mouse（カタログ番号S-KO-03801）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Prpf4b-KO
系統ID
KOCMP-19134-Prpf4b-B6J-VB
遺伝子名
製品ID
S-KO-03801
遺伝子別名
Prp4, Prpk, Prp4k, cbp143, 2610037H07
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 13
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000077853
NCBIトランスクリプトID
NM_013830
ターゲット領域
Exon 4~5
有効領域の大きさ
~2.6 kb
遺伝子研究の概要
Prpf4b, also known as PRP4K, is an essential kinase first identified in fission yeast and evolutionarily conserved. It is a pre-mRNA splicing regulator, phosphorylating spliceosomal components like PRPF6 and PRPF31 during spliceosome B complex formation. It is also involved in pathways such as transcription regulation and the spindle assembly checkpoint, playing an important role in maintaining chromosome stability [4,5].
In cancer research, several studies have implicated Prpf4b. In colon cancer, the phosphorylation level of PRPF4BS431p was significantly correlated with the overall survival of patients, suggesting a role in the disease's prognosis [1]. In lung adenocarcinoma, miR-224 was found to down-regulate PRPF4B, and this miRNA-mRNA pair was associated with lymph node metastasis and prognosis [2]. In hepatocellular carcinoma, miR-371-5p down-regulates PRPF4B, facilitating the G1/S transition and promoting tumor growth [3]. In triple-negative breast cancer, PRPF4B is essential for cancer cell migration and metastasis, and its depletion down-regulates genes in focal adhesion and ECM-interaction pathways [6]. In breast and ovarian cancer, PRP4K (PRPF4B) is a HER2-regulated modifier of taxane sensitivity, with low PRP4K levels correlating with taxane resistance [7]. Also, PRP4K is negatively regulated during epithelial-to-mesenchymal transition, and its depletion in normal mammary cells can increase cell invasion [8].
In conclusion, Prpf4b is crucial for pre-mRNA splicing and participates in multiple cellular pathways. Model-based research, especially in cancer, has shown its significance in tumorigenesis, metastasis, and treatment response. Understanding Prpf4b functions provides insights into disease mechanisms and potential therapeutic targets for various cancers.
References:
1. Gao, Li, Lu, Ying, Chen, Hai-Ning, Zhou, Zong-Guang, Dai, Lunzhi. 2023. Deciphering the Clinical Significance and Kinase Functions of GSK3α in Colon Cancer by Proteomics and Phosphoproteomics. In Molecular & cellular proteomics : MCP, 22, 100545. doi:10.1016/j.mcpro.2023.100545. https://pubmed.ncbi.nlm.nih.gov/37031867/
2. Wang, Yan, Shang, Shengtao, Yu, Kun, Ma, Wenduan, Zhao, Wei. 2020. miR-224, miR-147b and miR-31 associated with lymph node metastasis and prognosis for lung adenocarcinoma by regulating PRPF4B, WDR82 or NR3C2. In PeerJ, 8, e9704. doi:10.7717/peerj.9704. https://pubmed.ncbi.nlm.nih.gov/33282547/
3. Liu, Rui-Yan, Diao, Cai-Feng, Zhang, Yi, Liu, Min, Tang, Hua. 2013. miR-371-5p down-regulates pre mRNA processing factor 4 homolog B (PRPF4B) and facilitates the G1/S transition in human hepatocellular carcinoma cells. In Cancer letters, 335, 351-60. doi:10.1016/j.canlet.2013.02.045. https://pubmed.ncbi.nlm.nih.gov/23466643/
4. Corkery, Dale P, Holly, Alice C, Lahsaee, Sara, Dellaire, Graham. 2015. Connecting the speckles: Splicing kinases and their role in tumorigenesis and treatment response. In Nucleus (Austin, Tex.), 6, 279-88. doi:10.1080/19491034.2015.1062194. https://pubmed.ncbi.nlm.nih.gov/26098145/
5. Habib, Elias B, Mathavarajah, Sabateeshan, Dellaire, Graham. 2022. Tinker, Tailor, Tumour Suppressor: The Many Functions of PRP4K. In Frontiers in genetics, 13, 839963. doi:10.3389/fgene.2022.839963. https://pubmed.ncbi.nlm.nih.gov/35281802/
6. Koedoot, Esmee, Fokkelman, Michiel, Rogkoti, Vasiliki-Maria, Martens, John W M, van de Water, Bob. 2019. Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes. In Nature communications, 10, 2983. doi:10.1038/s41467-019-11020-3. https://pubmed.ncbi.nlm.nih.gov/31278301/
7. Corkery, Dale P, Le Page, Cécile, Meunier, Liliane, Mes-Masson, Anne-Marie, Dellaire, Graham. . PRP4K is a HER2-regulated modifier of taxane sensitivity. In Cell cycle (Georgetown, Tex.), 14, 1059-69. doi:10.1080/15384101.2015.1007775. https://pubmed.ncbi.nlm.nih.gov/25602630/
8. Clarke, Livia E, Cook, Allyson, Mathavarajah, Sabateeshan, Lewis, Stephen M, Dellaire, Graham. . Haploinsufficient tumor suppressor PRP4K is negatively regulated during epithelial-to-mesenchymal transition. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35, e22001. doi:10.1096/fj.202001063R. https://pubmed.ncbi.nlm.nih.gov/34674320/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。