Fgl1-KO Mouse
一般名
Fgl1-KO
製品ID
S-KO-06692
背景情報
C57BL/6NCya
系統ID
KOCMP-234199-Fgl1-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Fgl1-KO Mouse（カタログ番号S-KO-06692）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Fgl1-KO
系統ID
KOCMP-234199-Fgl1-B6N-VA
遺伝子名
製品ID
S-KO-06692
遺伝子別名
Mfire1
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conventional knockout
染色体
Chr 8
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000034003
NCBIトランスクリプトID
NM_145594
ターゲット領域
Exon 3~4
有効領域の大きさ
~9.4 kb
遺伝子研究の概要
Fgl1, or fibrinogen-like protein 1, is a protein secreted mainly by the liver. It is involved in multiple biological functions including regulating hepcidin and iron metabolism by antagonizing BMP signaling during anemia recovery in mice [5]. It also plays a crucial role in the immune system as a ligand of lymphocyte activation gene 3 (LAG3), and their interaction is associated with immune cell infiltration, proliferation, and secretion [1,2,6]. In addition, Fgl1 is related to cell proliferation and metabolism, and its expression is linked to various diseases, especially cancers [2].
In metastatic colorectal cancer, Fgl1 secreted from cancer cells and hepatocytes reduces T-cell infiltration, facilitating tumor progression. Disrupting the TAM-OTUD1-FGL1 axis, which upregulates FGL1 stability, inhibits metastatic tumor progression [3]. In non-small cell lung cancer (NSCLC), high FGL1 expression confers resistance to EGFR-TKI and gefitinib, and FGL1 knockdown enhances apoptosis induced by gefitinib [1,4]. Also, in NSCLC, Fgl1 promotes tumor cell proliferation, migration, and invasion through the KDM4A/STAT3 transcription mechanism, and its dynamic expression changes on circulating tumor cells can be a biomarker for predicting treatment efficacy [7]. In systemic lupus erythematosus (SLE) with liver damage, up-regulated Fgl1 from the injured liver, through the FGL1-LAG3 axis, impairs the suppressive function of regulatory T cells [8].
In conclusion, Fgl1 is a multifunctional protein involved in iron metabolism, immune regulation, and cancer progression. Mouse models, especially those disrupting relevant axes like the TAM-OTUD1-FGL1 axis in cancer, have revealed its role in disease development. These findings suggest that targeting Fgl1 could be a potential therapeutic strategy for various diseases, including cancers and SLE-related liver damage.
References:
1. Shi, An-Ping, Tang, Xi-Yang, Xiong, Yan-Lu, Ma, Nan, Zhao, Jin-Bo. 2022. Immune Checkpoint LAG3 and Its Ligand FGL1 in Cancer. In Frontiers in immunology, 12, 785091. doi:10.3389/fimmu.2021.785091. https://pubmed.ncbi.nlm.nih.gov/35111155/
2. Qian, Wenjing, Zhao, Mingfang, Wang, Ruoyu, Li, Heming. 2021. Fibrinogen-like protein 1 (FGL1): the next immune checkpoint target. In Journal of hematology & oncology, 14, 147. doi:10.1186/s13045-021-01161-8. https://pubmed.ncbi.nlm.nih.gov/34526102/
3. Li, Jia-Jun, Wang, Jin-Hong, Tian, Tian, Xu, Rui-Hua, Ju, Huai-Qiang. 2023. The liver microenvironment orchestrates FGL1-mediated immune escape and progression of metastatic colorectal cancer. In Nature communications, 14, 6690. doi:10.1038/s41467-023-42332-0. https://pubmed.ncbi.nlm.nih.gov/37872170/
4. Sun, Cuilan, Gao, Weiwei, Liu, Jiatao, Cheng, Hao, Hao, Jiqing. 2020. FGL1 regulates acquired resistance to Gefitinib by inhibiting apoptosis in non-small cell lung cancer. In Respiratory research, 21, 210. doi:10.1186/s12931-020-01477-y. https://pubmed.ncbi.nlm.nih.gov/32778129/
5. Sardo, Ugo, Perrier, Prunelle, Cormier, Kevin, Ganz, Tomas, Kautz, Léon. . The hepatokine FGL1 regulates hepcidin and iron metabolism during anemia in mice by antagonizing BMP signaling. In Blood, 143, 1282-1292. doi:10.1182/blood.2023022724. https://pubmed.ncbi.nlm.nih.gov/38232308/
6. Wang, Jun, Sanmamed, Miguel F, Datar, Ila, Schalper, Kurt, Chen, Lieping. 2018. Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. In Cell, 176, 334-347.e12. doi:10.1016/j.cell.2018.11.010. https://pubmed.ncbi.nlm.nih.gov/30580966/
7. Liu, Tian Yao, Yan, Jin Shan, Li, Xin, Zhao, Yue, Zhao, Ming Fang. 2024. FGL1: a novel biomarker and target for non-small cell lung cancer, promoting tumor progression and metastasis through KDM4A/STAT3 transcription mechanism. In Journal of experimental & clinical cancer research : CR, 43, 213. doi:10.1186/s13046-024-03140-6. https://pubmed.ncbi.nlm.nih.gov/39085849/
8. Chen, Kang, Li, Xingyu, Shang, Yuqi, Ming, Siqi, Wu, Yongjian. 2023. FGL1-LAG3 axis impairs IL-10-Producing regulatory T cells associated with Systemic lupus erythematosus disease activity. In Heliyon, 9, e20806. doi:10.1016/j.heliyon.2023.e20806. https://pubmed.ncbi.nlm.nih.gov/37916085/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。