Ifi27-KO Mouse
一般名
Ifi27-KO
製品ID
S-KO-10347
背景情報
C57BL/6JCya
系統ID
KOCMP-52668-Ifi27-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Ifi27-KO Mouse（カタログ番号S-KO-10347）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Ifi27-KO
系統ID
KOCMP-52668-Ifi27-B6J-VA
遺伝子名
製品ID
S-KO-10347
遺伝子別名
ISG12a, Ifi27l1, D12Ertd647e, 1110013J02Rik, 2900026P10Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 12
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000085065
NCBIトランスクリプトID
NM_026790
ターゲット領域
Exon 3~7
有効領域の大きさ
~4.8 kb
遺伝子研究の概要
IFI27, also known as IFN-α-inducible protein 27, is an interferon-stimulated gene (ISG) that plays diverse roles in the immune response, metabolism, and disease [2,3,4,6,8]. It is involved in pathways such as the innate immune response, regulation of the viral life cycle, and JAK-STAT signaling pathway [2]. IFI27 has been associated with various diseases including viral infections, cancer, and inflammatory disorders, highlighting its potential as a biomarker and therapeutic target [2,3,4,5,8,9].
In adipocytes, Ifi27-knockout leads to reduced expression of key enzymes in the tricarboxylic acid cycle, subunits of the electron transport chain, and uncoupling protein 1 (Ucp1), resulting in impaired oxygen consumption and defective thermogenesis [1,7]. This indicates that Ifi27 is crucial for mitochondrial function and adipocyte thermogenic adaptation [1,7]. In the context of viral infections, overexpression of IFI27 positively regulates DENV-2 infection, while knockdown has the opposite effect [2]. In HIV-1 infection, IFI27 is upregulated and can suppress HIV-1 replication by degrading the viral gag protein through the ubiquitin-proteasome pathway [4].
In conclusion, IFI27 is a multifunctional gene. Through gene knockout studies in adipocytes, its importance in mitochondrial function and thermogenesis has been revealed. In viral infections, its role in modulating the infection process has been demonstrated, suggesting that it could be a potential therapeutic target for treating viral diseases like HIV and dengue [1,2,4,7].
References:
1. Cui, Xuan, Liu, Haojie, Shi, Ting, Tang, Qi-Qun, Pan, Dongning. 2023. IFI27 Integrates Succinate and Fatty Acid Oxidation to Promote Adipocyte Thermogenic Adaption. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2301855. doi:10.1002/advs.202301855. https://pubmed.ncbi.nlm.nih.gov/37544897/
2. Jiang, Cheng, He, Cong, Kan, Jing, Zhou, Tao, Yang, Yi. . Integrative bulk and single-cell transcriptome profiling analysis reveals IFI27 as a novel interferon-stimulated gene in dengue. In Journal of medical virology, 95, e28706. doi:10.1002/jmv.28706. https://pubmed.ncbi.nlm.nih.gov/36971141/
3. Huang, Huijuan, Lv, Jiannan, Huang, Yonglun, Li, Hongmian, Qin, Yaqin. . IFI27 is a potential therapeutic target for HIV infection. In Annals of medicine, 54, 314-325. doi:10.1080/07853890.2021.1995624. https://pubmed.ncbi.nlm.nih.gov/35068272/
4. He, Wen-Qiang, Pang, Wei, Li, Na, Yang, Liu-Meng, Zheng, Yong-Tang. 2024. IFI27 inhibits HIV-1 replication by degrading Gag protein through the ubiquitin-proteasome pathway. In Journal of virology, 98, e0135624. doi:10.1128/jvi.01356-24. https://pubmed.ncbi.nlm.nih.gov/39475279/
5. Shojaei, Maryam, Shamshirian, Amir, Monkman, James, Short, Kirsty R, Tang, Benjamin. 2023. IFI27 transcription is an early predictor for COVID-19 outcomes, a multi-cohort observational study. In Frontiers in immunology, 13, 1060438. doi:10.3389/fimmu.2022.1060438. https://pubmed.ncbi.nlm.nih.gov/36685600/
6. Villamayor, Laura, López-García, Darío, Rivero, Vanessa, Nogales, Aitor, DeDiego, Marta L. 2023. The IFN-stimulated gene IFI27 counteracts innate immune responses after viral infections by interfering with RIG-I signaling. In Frontiers in microbiology, 14, 1176177. doi:10.3389/fmicb.2023.1176177. https://pubmed.ncbi.nlm.nih.gov/37187533/
7. Jin, Weiwei, Jin, Wenfang, Pan, Dongning. 2018. Ifi27 is indispensable for mitochondrial function and browning in adipocytes. In Biochemical and biophysical research communications, 501, 273-279. doi:10.1016/j.bbrc.2018.04.234. https://pubmed.ncbi.nlm.nih.gov/29730295/
8. Shojaei, Maryam, McLean, Anthony S. 2025. Interferon-stimulated gene IFI27 as a multifaceted candidate target in precision medicine. In Trends in immunology, 46, 219-228. doi:10.1016/j.it.2025.01.008. https://pubmed.ncbi.nlm.nih.gov/40044530/
9. Zhang, Ying, Chen, Chuangzhen, Liu, Zhaoyong, Hu, Wang, Lin, Zhixiong. 2022. PABPC1-induced stabilization of IFI27 mRNA promotes angiogenesis and malignant progression in esophageal squamous cell carcinoma through exosomal miRNA-21-5p. In Journal of experimental & clinical cancer research : CR, 41, 111. doi:10.1186/s13046-022-02339-9. https://pubmed.ncbi.nlm.nih.gov/35346324/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。