Lpin3-KO Mouse

Lpin3, encoding phosphatidate phosphatase type 1 enzyme, is involved in the de novo biosynthesis of triacylglycerol, phosphatidylcholine, and phosphatidylethanolamine [3]. Lipins, including Lpin3, also have a role in transcriptional co-regulation [2]. It is widely expressed in tissues such as liver, small intestine, kidney, and adipose tissues in sheep [2].

In colorectal cancer, LPIN3 is highly expressed and promotes tumor growth. Knockdown of LPIN3 in vivo strengthened CD8+ T cell-mediated cytotoxicity, infiltration, and activation. LPIN3 may enhance tumor growth by activating the β-catenin signaling pathway and reduce tumor antigen presentation signals, suppressing CD8+ T cell-mediated cytotoxicity [1].

In summary, Lpin3 has important functions in lipid metabolism and transcriptional co-regulation. In colorectal cancer, studies (such as LPIN3 knockdown in vivo) have revealed its role in promoting tumor growth by influencing β-catenin signaling and CD8+ T cell activity, providing insights into the molecular mechanisms of colorectal cancer progression.

References:

1. Zhang, Xiaoming, Fang, Hao, Wu, Wenliang, Wang, Haizhou, Shi, Yifei. 2025. LPIN3 promotes colorectal cancer growth by dampening intratumoral CD8+ T cell effector function. In Cancer immunology, immunotherapy : CII, 74, 135. doi:10.1007/s00262-025-03989-2. https://pubmed.ncbi.nlm.nih.gov/40042548/

2. Jiao, Xiao-Li, Jing, Jiong-Jie, Qiao, Li-Ying, Jia, Xia-Li, Liu, Wen-Zhong. 2016. Ontogenetic Expression of Lpin2 and Lpin3 Genes and Their Associations with Traits in Two Breeds of Chinese Fat-tailed Sheep. In Asian-Australasian journal of animal sciences, 29, 333-42. doi:10.5713/ajas.15.0467. https://pubmed.ncbi.nlm.nih.gov/26950863/

3. He, Xiaoping, Xu, Xuewen, Liu, Bang. 2008. Molecular characterization, chromosomal localization and association analysis with back-fat thickness of porcine LPIN2 and LPIN3. In Molecular biology reports, 36, 1819-24. doi:10.1007/s11033-008-9385-2. https://pubmed.ncbi.nlm.nih.gov/18989753/