Mkrn2-KO Mouse
一般名
Mkrn2-KO
製品ID
S-KO-12060
背景情報
C57BL/6NCya
系統ID
KOCMP-67027-Mkrn2-B6N-VA
状況
このマウス系統を論文で使用する場合は、「Mkrn2-KO Mouse（カタログ番号S-KO-12060）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Mkrn2-KO
系統ID
KOCMP-67027-Mkrn2-B6N-VA
遺伝子名
製品ID
S-KO-12060
遺伝子別名
2610002L04Rik
遺伝子別名
C57BL/6NCya
NCBI ID
修正
Conventional knockout
染色体
Chr 6
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000000449
NCBIトランスクリプトID
NM_023290
ターゲット領域
Exon 2~4
有効領域の大きさ
~2.4 kb
遺伝子研究の概要
Mkrn2, an evolutionarily conserved gene, encodes an E3 ubiquitin-protein. It is involved in multiple biological processes, with its protein product having the ability to regulate protein degradation through ubiquitination. It plays a role in pathways such as those related to NF-κB-mediated inflammatory responses, mRNA nuclear export, and is potentially associated with many diseases [3,8]. Genetic models, especially knockout mouse models, have been crucial in studying Mkrn2's functions.
In mice, deletion of Mkrn2 leads to male infertility. This is due to decreased expression of STAT1, SIX4, and TNC, and abnormal apoptosis in the testis through the p53/PERP-mediated pathway [2,5]. In zebrafish, knockdown or knockout of MKRN2 affects retinal development by interacting with GLE1 to regulate mRNA nuclear export [3]. In cancer research, MKRN2 is down-regulated in gastric cancer, and its overexpression inhibits cell proliferation by promoting ubiquitination-mediated degradation of PKM2 [1]. In colorectal carcinoma, the LINC00294/miR-620/MKRN2 axis negatively regulates malignant progression [4]. In ovarian cancer, overexpression of MKRN2 reduces tumor-associated behavior and induces apoptosis [6]. In non-small-cell lung cancer, MKRN2 inhibits cell migration and invasion by negatively regulating the PI3K/Akt pathway [7]. However, in melanoma, MKRN2 promotes cell proliferation by ubiquitinating P53 [9].
In conclusion, Mkrn2 is essential for male fertility, retinal development, and plays diverse roles in various cancers. The gene knockout models in mice and zebrafish have been instrumental in uncovering these functions, highlighting its significance in understanding male infertility and cancer development mechanisms.
References:
1. Liu, Zheng, Xiang, Shuyao, Guo, Xingchen, Kou, Jiaxin, Zhang, Jun. 2022. MKRN2 inhibits the proliferation of gastric cancer by downregulating PKM2. In Aging, 14, 2004-2013. doi:10.18632/aging.203643. https://pubmed.ncbi.nlm.nih.gov/35196650/
2. Wang, Lin, Yong, Yan-Ling, Wang, Kun-Kun, Qiu, Jian-Ge, Jiang, Bing-Hua. 2023. MKRN2 knockout causes male infertility through decreasing STAT1, SIX4, and TNC expression. In Frontiers in endocrinology, 14, 1138096. doi:10.3389/fendo.2023.1138096. https://pubmed.ncbi.nlm.nih.gov/36967804/
3. Wolf, Eric J, Miles, Amanda, Lee, Eliza S, Tropepe, Vincent, Emili, Andrew. . MKRN2 Physically Interacts with GLE1 to Regulate mRNA Export and Zebrafish Retinal Development. In Cell reports, 31, 107693. doi:10.1016/j.celrep.2020.107693. https://pubmed.ncbi.nlm.nih.gov/32460013/
4. Qi, Puliang, Yexie, Zhihua, Xue, Chen, Zhao, Zhanxue, Zhang, Xikun. . LINC00294/miR-620/MKRN2 axis provides biomarkers and negatively regulates malignant progression in colorectal carcinoma. In Human & experimental toxicology, 42, 9603271231167577. doi:10.1177/09603271231167577. https://pubmed.ncbi.nlm.nih.gov/37218161/
5. Qian, Ying-Chen, Xie, Yun-Xia, Wang, Chao-Shan, Wang, Lin, Jiang, Bing-Hua. . Mkrn2 deficiency induces teratozoospermia and male infertility through p53/PERP-mediated apoptosis in testis. In Asian journal of andrology, 22, 414-421. doi:10.4103/aja.aja_76_19. https://pubmed.ncbi.nlm.nih.gov/31489847/
6. Jiang, F Z, Xia, Q J, Wu, L, Zhang, Y M. . [Overexpression of MKRN2 Inhibits the Growth of Ovarian Cancer Cells]. In Molekuliarnaia biologiia, 57, 687-688. doi:. https://pubmed.ncbi.nlm.nih.gov/37528788/
7. Jiang, Jun, Xu, Yitong, Ren, Hongjiu, Jiang, Lihong, Qiu, Xueshan. 2018. MKRN2 inhibits migration and invasion of non-small-cell lung cancer by negatively regulating the PI3K/Akt pathway. In Journal of experimental & clinical cancer research : CR, 37, 189. doi:10.1186/s13046-018-0855-7. https://pubmed.ncbi.nlm.nih.gov/30103781/
8. Shin, Chanyoung, Ito, Yuma, Ichikawa, Shota, Sakata-Sogawa, Kumiko, Tanaka, Takashi. 2017. MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses. In Scientific reports, 7, 46097. doi:10.1038/srep46097. https://pubmed.ncbi.nlm.nih.gov/28378844/
9. Zhang, Yiling, Cui, Ningning, Zheng, Gang. 2020. Ubiquitination of P53 by E3 ligase MKRN2 promotes melanoma cell proliferation. In Oncology letters, 19, 1975-1984. doi:10.3892/ol.2020.11261. https://pubmed.ncbi.nlm.nih.gov/32194692/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。