Zdhhc3-KO Mouse
一般名
Zdhhc3-KO
製品ID
S-KO-12921
背景情報
C57BL/6JCya
系統ID
KOCMP-69035-Zdhhc3-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Zdhhc3-KO Mouse（カタログ番号S-KO-12921）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Zdhhc3-KO
系統ID
KOCMP-69035-Zdhhc3-B6J-VA
遺伝子名
製品ID
S-KO-12921
遺伝子別名
GODZ, DHHC-3, Gramp1, Zfp373, 1110020O22Rik, 1810006O10Rik, 2210017C02Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 9
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000238520
NCBIトランスクリプトID
NM_001372538
ターゲット領域
Exon 4~5
有効領域の大きさ
~0.8 kb
遺伝子研究の概要
ZDHHC3, Zinc finger DHHC-type palmitoyltransferase 3, is a key enzyme responsible for catalyzing S-palmitoylation, a post-translational lipid modification. Palmitoylation is involved in diverse cellular processes such as protein trafficking, membrane localization, and signal transduction [1,2,3,4,5,6,7,8]. It plays a role in multiple biological pathways and is of great biological importance in various tissues and organisms [1-10]. Genetic models, like KO/CKO mouse models, are valuable tools for studying its functions.
In non-alcoholic steatohepatitis (NASH), hepatocyte-specific dysfunction of ZDHHC3 significantly inhibits palmitoylated IRHOM2 deposition, suppressing fatty-acids-mediated hepatosteatosis and inflammation in vitro and NASH pathological phenotypes in rodent and rabbit models [1]. In hepatocellular carcinoma (HCC), the small-molecule inhibitor of ZDHHC3 combined with anti-PD-1 immunotherapy inhibits DEN/CCl4-induced HCC growth in mice, as ZDHHC3-mediated SCAP S-acylation promotes cholesterol biosynthesis and tumor immune escape [2]. In the central nervous system, genetic deletion of ZDHHC3 results in decreased Cadm4 palmitoylation and defects in CNS myelination, phenocopying the Cadm4-KI mouse model [3]. In kidney clear cell carcinoma (KIRC), zDHHC3 suppression leads to decreased S-palmitoylation of SLC9A2 and inhibits Caki-2 cell apoptosis [4].
In conclusion, ZDHHC3 is crucial for S-palmitoylation-mediated biological processes. Model-based research, especially KO/CKO mouse models, has revealed its role in diseases like NASH, HCC, CNS demyelination diseases, and KIRC. Understanding ZDHHC3 functions provides insights into disease mechanisms and potential therapeutic targets for these diseases.
References:
1. Xu, Minxuan, Tan, Jun, Zhu, Liancai, Zou, Benkui, Wang, Bochu. 2023. Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S-Palmitoylated IRHOM2. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2302130. doi:10.1002/advs.202302130. https://pubmed.ncbi.nlm.nih.gov/37544908/
2. Wu, Mingzhi, Zhou, Xiaojun, Zhou, Xinyi, Wang, Fubing, Li, Youjun. 2024. ZDHHC3-mediated SCAP S-acylation promotes cholesterol biosynthesis and tumor immune escape in hepatocellular carcinoma. In Cell reports, 43, 114962. doi:10.1016/j.celrep.2024.114962. https://pubmed.ncbi.nlm.nih.gov/39522165/
3. Chang, Yanli, Zhu, Jiangli, Li, Xiaopeng, Wang, Xiaoning, Kong, Eryan. 2024. Palmitoylation regulates myelination by modulating the ZDHHC3-Cadm4 axis in the central nervous system. In Signal transduction and targeted therapy, 9, 254. doi:10.1038/s41392-024-01971-5. https://pubmed.ncbi.nlm.nih.gov/39327467/
4. Zhang, Xiuyun, Hou, Junpeng, Zhou, Guangyuan, Wang, Haixia, Wu, Zeang. 2024. zDHHC3-mediated S-palmitoylation of SLC9A2 regulates apoptosis in kidney clear cell carcinoma. In Journal of cancer research and clinical oncology, 150, 194. doi:10.1007/s00432-024-05737-y. https://pubmed.ncbi.nlm.nih.gov/38619631/
5. Yao, Han, Lan, Jiang, Li, Chushu, Cui, Yun, Xu, Jie. 2019. Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours. In Nature biomedical engineering, 3, 306-317. doi:10.1038/s41551-019-0375-6. https://pubmed.ncbi.nlm.nih.gov/30952982/
6. Jing, Huiyuan, Liu, Ying, Song, Yvzhen, Liu, Jie, Zhao, Pandeng. 2025. ZDHHC3-LYPLA1 regulates PRRSV-2 replication through reversible palmitoylation. In Veterinary microbiology, 301, 110368. doi:10.1016/j.vetmic.2025.110368. https://pubmed.ncbi.nlm.nih.gov/39787744/
7. Lievens, Patricia Marie-Jeanne, Kuznetsova, Tatiana, Kochlamazashvili, Gaga, Ponimaskin, Evgeni, Dityatev, Alexander. 2016. ZDHHC3 Tyrosine Phosphorylation Regulates Neural Cell Adhesion Molecule Palmitoylation. In Molecular and cellular biology, 36, 2208-25. doi:10.1128/MCB.00144-16. https://pubmed.ncbi.nlm.nih.gov/27247265/
8. Baldwin, Tanya A, Teuber, James P, Kuwabara, Yasuhide, Brody, Matthew J, Molkentin, Jeffery D. 2023. Palmitoylation-dependent regulation of cardiomyocyte Rac1 signaling activity and minor effects on cardiac hypertrophy. In The Journal of biological chemistry, 299, 105426. doi:10.1016/j.jbc.2023.105426. https://pubmed.ncbi.nlm.nih.gov/37926281/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
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グローバル由来:
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