Dnajc25-KO Mouse

Dnajc25, a member of the DnaJ/Hsp40 subfamily of the heat shock protein family, acts as a co-chaperone. It binds to the chaperone Hsp70 via its J domain, stimulating ATP hydrolysis to assist in protein translation, folding, unfolding, translocation, and degradation [1]. It may be involved in pathways related to protein-handling processes and has significance in various biological processes and disease-related mechanisms.

In hepatocellular carcinoma (HCC), DnaJC25 is down-regulated compared to adjacent normal tissues. Overexpression of DnaJC25 inhibits colony growth in both quantity and size and significantly increases cell apoptosis, indicating its potential as a tumor suppressor gene in hepatocellular carcinogenesis [1]. RNF149, an E3 ubiquitin ligase, promotes HCC progression and has been found to target DnaJC25 as a new substrate [2]. In the acidic environment of tumors, DnaJC25 expression is down-regulated, which may be related to the regulation of tumor-associated functional parameters [3]. In catfish, DnaJC25 is associated with heat stress, being involved in the protein-folding process related to this stress response [4].

In summary, DnaJC25 plays important roles in processes such as tumorigenesis and stress responses. Its down-regulation in HCC and the impact of its interaction with RNF149 highlight its significance in liver cancer research. The down-regulation in an acidic tumor environment and its role in catfish heat stress also show its diverse functions across different biological contexts. [1,2,3,4]

References:

1. Liu, Tingting, Jiang, Wei, Han, Dingding, Yu, Long. 2012. DNAJC25 is downregulated in hepatocellular carcinoma and is a novel tumor suppressor gene. In Oncology letters, 4, 1274-1280. doi:. https://pubmed.ncbi.nlm.nih.gov/23205125/

2. Guo, Zhaoyu, Jiang, Pei, Dong, Qian, Tian, Chunyan, Sun, Aihua. 2023. RNF149 Promotes HCC Progression through Its E3 Ubiquitin Ligase Activity. In Cancers, 15, . doi:10.3390/cancers15215203. https://pubmed.ncbi.nlm.nih.gov/37958377/

3. Rauschner, Mandy, Lange, Luisa, Hüsing, Thea, Thews, Oliver, Riemann, Anne. 2021. Impact of the acidic environment on gene expression and functional parameters of tumors in vitro and in vivo. In Journal of experimental & clinical cancer research : CR, 40, 10. doi:10.1186/s13046-020-01815-4. https://pubmed.ncbi.nlm.nih.gov/33407762/

4. Jin, Y, Zhou, T, Geng, X, Su, B, Liu, Z. 2016. A genome-wide association study of heat stress-associated SNPs in catfish. In Animal genetics, 48, 233-236. doi:10.1111/age.12482. https://pubmed.ncbi.nlm.nih.gov/27476875/