Cartpt-KO Mouse
一般名
Cartpt-KO
製品ID
S-KO-16516
背景情報
C57BL/6JCya
系統ID
KOCMP-27220-Cartpt-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Cartpt-KO Mouse（カタログ番号S-KO-16516）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Cartpt-KO
系統ID
KOCMP-27220-Cartpt-B6J-VB
遺伝子名
製品ID
S-KO-16516
遺伝子別名
Cart
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 13
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000022150
NCBIトランスクリプトID
NM_013732
ターゲット領域
Exon 2~3
有効領域の大きさ
~2.8 kb
遺伝子研究の概要
Cartpt, encoding the cocaine-and amphetamine-regulated transcript prepropeptide, is involved in multiple biological processes. It plays roles in reward and feeding behavior, with functional characteristics similar to an endogenous psychostimulant [7]. It also participates in various physiological and pathological regulations, including those related to metabolism, cardiovascular and skeletal development, and ovarian follicular development [3,4,5,6,2].
In male human thoracic aortic aneurysm (TAA) samples, a spatially distinct CARTPT-expressing smooth muscle cell (SMC) subtype was identified. Functional studies suggest that human CART, the product of Cartpt, promotes the osteochondrogenic switch of aortic SMCs, potentially leading to medial calcification of the thoracic aorta [1]. In preosteoblast-specific Atrx conditional knockout mice, Atrx deletion led to high levels of Cartpt expression in osteolineage cells. Mechanistically, ATRX suppresses Cartpt transcription by binding to the promoter. Cartpt silencing in these cells rescued the molecular phenotype by increasing the Rankl/Opg expression ratio, indicating Cartpt's role in skeletal development [2].
In summary, Cartpt is involved in diverse biological functions including reward processing, cardiovascular and skeletal development, and ovarian follicular regulation. Studies using gene knockout or conditional knockout mouse models, like the Atrx conditional knockout mouse model, have provided insights into Cartpt's role in skeletal development and its potential implications in diseases such as thoracic aortic aneurysms. These findings contribute to understanding the molecular mechanisms underlying these biological processes and diseases [1,2].
References:
1. Mizrak, Dogukan, Zhao, Yang, Feng, Hao, Yang, Bo, Chen, Y Eugene. 2023. Single-Molecule Spatial Transcriptomics of Human Thoracic Aortic Aneurysms Uncovers Calcification-Related CARTPT-Expressing Smooth Muscle Cells. In Arteriosclerosis, thrombosis, and vascular biology, 43, 2285-2297. doi:10.1161/ATVBAHA.123.319329. https://pubmed.ncbi.nlm.nih.gov/37823268/
2. Chen, Yi-Ting, Jiang, Ming-Ming, Leynes, Carolina, Bae, Yangjin, Lee, Brendan. 2025. ATRX silences Cartpt expression in osteoblastic cells during skeletal development. In The Journal of clinical investigation, 135, . doi:10.1172/JCI163587. https://pubmed.ncbi.nlm.nih.gov/39744954/
3. Smith, G W, Sen, A, Folger, J K, Ireland, J J. . Putative role of cocaine- and amphetamine-regulated transcript (CARTPT) in dominant follicle selection in cattle. In Society of Reproduction and Fertility supplement, 67, 105-17. doi:. https://pubmed.ncbi.nlm.nih.gov/21755666/
4. Mahmoudi-Nezhad, Mahsa, Farhangi, Mahdieh Abbasalizad, Kahroba, Houman. 2020. Cocaine and amphetamine-regulated transcript prepropeptide gene (CARTPT) polymorphism interacts with Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI) to affect hypothalamic hormones and cardio-metabolic risk factors among obese individuals. In Journal of translational medicine, 18, 16. doi:10.1186/s12967-020-02208-z. https://pubmed.ncbi.nlm.nih.gov/31918705/
5. Khodarahmi, Mahdieh, Siri, Goli, Erahimzadeh, Farnoosh, Farhangi, Mahdieh Abbasalizad, Shanehbandi, Dariush. 2022. Dietary glycemic index and glycemic load mediate the effect of CARTPT rs2239670 gene polymorphism on metabolic syndrome and metabolic risk factors among adults with obesity. In BMC endocrine disorders, 22, 288. doi:10.1186/s12902-022-01188-z. https://pubmed.ncbi.nlm.nih.gov/36404325/
6. Mahmoudinezhad, Mahsa, Abbasalizad-Farhangi, Mahdieh, Kahroba, Houman. 2021. Hypothalamic-pituitary hormones will be affected by the interaction between 5q13-14-rs2239670 (CARTPT) gene variants and diet in different obesity phenotypes. In BMC research notes, 14, 443. doi:10.1186/s13104-021-05857-5. https://pubmed.ncbi.nlm.nih.gov/34876220/
7. Lohoff, Falk W, Bloch, Paul J, Weller, Andrew E, O'Brien, Charles P, Berrettini, Wade H. 2008. Genetic variants in the cocaine- and amphetamine-regulated transcript gene (CARTPT) and cocaine dependence. In Neuroscience letters, 440, 280-3. doi:10.1016/j.neulet.2008.05.073. https://pubmed.ncbi.nlm.nih.gov/18572320/
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