Pycr1-KO Mouse
一般名
Pycr1-KO
製品ID
S-KO-17111
背景情報
C57BL/6JCya
系統ID
KOCMP-209027-Pycr1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Pycr1-KO Mouse（カタログ番号S-KO-17111）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Pycr1-KO
系統ID
KOCMP-209027-Pycr1-B6J-VA
遺伝子名
製品ID
S-KO-17111
遺伝子別名
P5CR1, P5CR 1
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000170556
NCBIトランスクリプトID
NM_001379085.1
ターゲット領域
Exon 3~6
有効領域の大きさ
~2.1 kb
遺伝子研究の概要
Pycr1, or pyrroline-5-carboxylate reductase 1, is a key enzyme in proline biosynthesis, catalyzing the final step of converting pyrroline-5-carboxylate (P5C) to proline [9]. Proline metabolism is involved in multiple cellular processes, and Pycr1 has been associated with maintaining redox balance and preventing apoptosis [10]. Mouse models have been crucial in understanding its function.
In various cancers, Pycr1 has been shown to play oncogenic roles. In colorectal cancer, under hypoxia, nuclear IGF1R phosphorylates Pycr1 at Tyrosine 135, promoting its binding to ELK4 and subsequent recruitment to ELK4-targeted genes' promoters, which sustains tumor growth [1]. In breast cancer, cancer-associated fibroblasts (CAFs) rely on Pycr1-mediated proline synthesis for the deposition of pro-tumorigenic extracellular matrix. Reducing Pycr1 levels in CAFs decreased tumour collagen production, growth, and metastatic spread [2]. In liver, bladder, pancreatic, papillary renal cell, and lung cancers, knockdown or inhibition of Pycr1 led to decreased cell proliferation, invasion, and metastasis, and increased apoptosis, often through affecting signaling pathways like Akt/Wnt/β-catenin, PI3K/AKT/mTOR, and JAK-STAT3 [3,4,6,7,8]. In allergic asthma, Pycr1 knockout in a murine model decreased proline in lung tissues, reducing airway remodeling and epithelial-mesenchymal transition (EMT) [5].
In conclusion, Pycr1 is essential for proline biosynthesis and has significant impacts on various biological processes. Studies using gene knockout models in mice have revealed its oncogenic role in multiple cancers and its contribution to airway remodeling in allergic asthma, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Zheng, Ke, Sha, Nannan, Hou, Guofang, Jiang, Yuhui, Chen, Tao. 2023. IGF1R-phosphorylated PYCR1 facilitates ELK4 transcriptional activity and sustains tumor growth under hypoxia. In Nature communications, 14, 6117. doi:10.1038/s41467-023-41658-z. https://pubmed.ncbi.nlm.nih.gov/37777542/
2. Kay, Emily J, Paterson, Karla, Riera-Domingo, Carla, Zagnoni, Michele, Zanivan, Sara. 2022. Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix. In Nature metabolism, 4, 693-710. doi:10.1038/s42255-022-00582-0. https://pubmed.ncbi.nlm.nih.gov/35760868/
3. Wang, Haoyu, Xu, Mu, Zhang, Tong, Wang, Qian, Li, John Zhong. . PYCR1 promotes liver cancer cell growth and metastasis by regulating IRS1 expression through lactylation modification. In Clinical and translational medicine, 14, e70045. doi:10.1002/ctm2.70045. https://pubmed.ncbi.nlm.nih.gov/39422696/
4. Du, Shuangkuan, Sui, Yongjie, Ren, Wei, Zhou, Jiancheng, Du, Chun. 2021. PYCR1 promotes bladder cancer by affecting the Akt/Wnt/β-catenin signaling. In Journal of bioenergetics and biomembranes, 53, 247-258. doi:10.1007/s10863-021-09887-3. https://pubmed.ncbi.nlm.nih.gov/33689096/
5. Xu, Tingting, Wu, Zhenzhen, Yuan, Qi, Huang, Mao, Ji, Ningfei. 2023. Proline is increased in allergic asthma and promotes airway remodeling. In JCI insight, 8, . doi:10.1172/jci.insight.167395. https://pubmed.ncbi.nlm.nih.gov/37432745/
6. Wang, Huanyu, Mao, Weilin, Lou, Wenhui, Xu, Xuefeng, Zhang, Lei. 2022. PYCR1: A Potential Prognostic Biomarker in Pancreatic Ductal Adenocarcinoma. In Journal of Cancer, 13, 1501-1511. doi:10.7150/jca.61498. https://pubmed.ncbi.nlm.nih.gov/35371311/
7. Wang, Qiu-Li, Liu, Ling. 2019. PYCR1 is Associated with Papillary Renal Cell Carcinoma Progression. In Open medicine (Warsaw, Poland), 14, 586-592. doi:10.1515/med-2019-0066. https://pubmed.ncbi.nlm.nih.gov/31428683/
8. Zhang, Lihong, Zhao, Xinyu, Wang, Enqin, Xu, Hongkun, Zhang, Baojun. 2023. PYCR1 promotes the malignant progression of lung cancer through the JAK-STAT3 signaling pathway via PRODH-dependent glutamine synthesize. In Translational oncology, 32, 101667. doi:10.1016/j.tranon.2023.101667. https://pubmed.ncbi.nlm.nih.gov/37018868/
9. Stum, Morgane G, Tadenev, Abigail L D, Seburn, Kevin L, John, Simon W M, Burgess, Robert W. . Genetic analysis of Pycr1 and Pycr2 in mice. In Genetics, 218, . doi:10.1093/genetics/iyab048. https://pubmed.ncbi.nlm.nih.gov/33734376/
10. Milne, Kirsty, Sun, Jianhui, Zaal, Esther A, Jamieson, Craig, Agami, Reuven. 2019. A fragment-like approach to PYCR1 inhibition. In Bioorganic & medicinal chemistry letters, 29, 2626-2631. doi:10.1016/j.bmcl.2019.07.047. https://pubmed.ncbi.nlm.nih.gov/31362921/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
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