Krt15-KO Mouse
一般名
Krt15-KO
製品ID
S-KO-17220
背景情報
C57BL/6JCya
系統ID
KOCMP-16665-Krt15-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Krt15-KO Mouse（カタログ番号S-KO-17220）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Krt15-KO
系統ID
KOCMP-16665-Krt15-B6J-VB
遺伝子名
製品ID
S-KO-17220
遺伝子別名
K15, Krt1-15
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000107411
NCBIトランスクリプトID
NM_008469.2
ターゲット領域
Exon 2~7
有効領域の大きさ
~1935 bp
遺伝子研究の概要
Krt15, a type I keratin lacking a defined type II partner, plays a key role in maintaining cytoplasmic stability. It has been associated with various biological processes and disease conditions. In the context of some tissues, it may mark stem or progenitor cells, and is involved in processes like cell migration, invasion, and differentiation [2,5,6,8].
In colorectal cancer, KRT15 acts as an oncogene. Ectopic expression of KRT15 promotes cell migration and invasion, while silencing it suppresses these processes. This occurs at least partly through the β -catenin/MMP-7 signaling pathway, suggesting it as a potential therapeutic target for metastatic colorectal cancer [1].
In breast cancer, its role seems to be context-dependent. In invasive breast carcinoma, low KRT15 expression is associated with poor prognosis, and high expression is linked to immune-cell infiltration [4]. In early breast cancer screening, KRT15 assessment may be helpful, as it shows different correlations with clinical characteristics among different breast cancer subtypes [3].
In the lung, Krt15 protects against cigarette smoke-induced epithelial mesenchymal transformation by regulating MMP-9 expression, and Krt15+ cells can transform into alveolar type II cells to protect alveoli, suggesting it as a potential therapeutic target for COPD [7].
In the intestine, Krt15 labels long-lived, multipotent, and radio-resistant crypt cells, and loss of the tumor suppressor Apc in Krt15+ cells leads to adenoma and adenocarcinoma formation [8].
In conclusion, Krt15 has diverse functions in maintaining cellular stability, differentiation, and tissue homeostasis. Its role in cancer progression, especially in colorectal, breast, and intestinal cancers, and in lung diseases like COPD, is significant. Gene knockout and other functional studies in mouse models have been crucial in revealing these roles, providing insights into potential therapeutic strategies for these diseases.
References:
1. Chen, Weida, Miao, Chengli. 2022. KRT15 promotes colorectal cancer cell migration and invasion through β-catenin/MMP-7 signaling pathway. In Medical oncology (Northwood, London, England), 39, 68. doi:10.1007/s12032-021-01619-2. https://pubmed.ncbi.nlm.nih.gov/35477819/
2. Rao, X, Wang, J, Song, H M, Deng, B, Li, J G. 2019. KRT15 overexpression predicts poor prognosis in colorectal cancer. In Neoplasma, 67, 410-414. doi:10.4149/neo_2019_190531N475. https://pubmed.ncbi.nlm.nih.gov/31884802/
3. Zhang, Zijing, Wang, Hongying, Jin, Yiting, Zou, Liping, Zou, Qiang. 2023. KRT15 in early breast cancer screening and correlation with HER2 positivity, pathological grade and N stage. In Biomarkers in medicine, 17, 553-562. doi:10.2217/bmm-2023-0130. https://pubmed.ncbi.nlm.nih.gov/37814985/
4. Zhong, Pengcheng, Shu, Rong, Wu, Huiwen, Shen, Xiaoling, Hu, Yingjie. 2021. Low KRT15 expression is associated with poor prognosis in patients with breast invasive carcinoma. In Experimental and therapeutic medicine, 21, 305. doi:10.3892/etm.2021.9736. https://pubmed.ncbi.nlm.nih.gov/33717248/
5. Busslinger, Georg A, Weusten, Bas L A, Bogte, Auke, Brosens, Lodewijk A A, Clevers, Hans. . Human gastrointestinal epithelia of the esophagus, stomach, and duodenum resolved at single-cell resolution. In Cell reports, 34, 108819. doi:10.1016/j.celrep.2021.108819. https://pubmed.ncbi.nlm.nih.gov/33691112/
6. Ievlev, Vitaly, Lynch, Thomas J, Freischlag, Kyle W, Engelhardt, John F, Parekh, Kalpaj R. 2023. Krt14 and Krt15 differentially regulate regenerative properties and differentiation potential of airway basal cells. In JCI insight, 8, . doi:10.1172/jci.insight.162041. https://pubmed.ncbi.nlm.nih.gov/36512409/
7. Zhu, Wensi, Han, Linxiao, Wu, Yuanyuan, Zhu, Qiaoliang, Zhou, Jian. 2023. Keratin 15 protects against cigarette smoke-induced epithelial mesenchymal transformation by MMP-9. In Respiratory research, 24, 297. doi:10.1186/s12931-023-02598-w. https://pubmed.ncbi.nlm.nih.gov/38007424/
8. Giroux, Véronique, Stephan, Julien, Chatterji, Priya, Hamilton, Kathryn E, Rustgi, Anil K. 2018. Mouse Intestinal Krt15+ Crypt Cells Are Radio-Resistant and Tumor Initiating. In Stem cell reports, 10, 1947-1958. doi:10.1016/j.stemcr.2018.04.022. https://pubmed.ncbi.nlm.nih.gov/29805107/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
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