Dmc1-KO Mouse
一般名
Dmc1-KO
製品ID
S-KO-17344
背景情報
C57BL/6JCya
系統ID
KOCMP-13404-Dmc1-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Dmc1-KO Mouse（カタログ番号S-KO-17344）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Dmc1-KO
系統ID
KOCMP-13404-Dmc1-B6J-VB
遺伝子名
製品ID
S-KO-17344
遺伝子別名
sgdp, Dmc1h, Lim15, Mei11
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 15
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000023065
NCBIトランスクリプトID
NM_010059
ターゲット領域
Exon 6~7
有効領域の大きさ
~2.2 kb
遺伝子研究の概要
Dmc1, short for disruption Meiotic cDNA 1, is a meiosis-specific recombinase. Meiotic recombination, which is crucial for fertility in most sexually-reproducing organisms, involves the repair of programmed DNA double-strand breaks. Dmc1, along with RAD51, is essential in this process as they search for and invade an intact DNA molecule on the homologous chromosome [1,2,3,5].
In Arabidopsis, Dmc1 attenuates RAD51-mediated recombination. Ectopic expression of Dmc1 in somatic cells makes plants hypersensitive to DNA damage and impairs RAD51-dependent homologous recombination. Also, in meiosis, a dominant-negative Dmc1 protein impairs RAD51-mediated DSB repair, suggesting Dmc1 acts to prevent RAD51-mediated recombination and this requires local assembly of Dmc1 nucleofilaments [1]. In mice, germ-line-specific conditional knockout (cKO) of FIGNL1 led to defective chromosome synapsis and impaired meiotic DSB repair with the accumulation of RAD51/DMC1 on meiotic chromosomes, indicating FIGNL1's role in homologous recombination at a post-assembly stage of RAD51/DMC1 filaments [4]. Similarly, both FIGNL1 and FIRRM are required for completing meiotic prophase in mouse spermatocytes, and the FIGNL1-FIRRM complex limits RAD51 and DMC1 accumulation on intact chromatin [7]. In wheat, CRISPR-Cas9-generated Dmc1-D1 mutants showed a significant decrease in crossovers at both high and low temperatures, indicating Dmc1-D1 is responsible for preserving normal crossover formation under such temperature conditions [6].
In conclusion, Dmc1 is vital for meiotic recombination, playing a key role in processes like preventing RAD51-mediated recombination, facilitating chromosome synapsis and crossover formation. The use of gene knockout and conditional knockout mouse models, as well as other genetic models like the wheat CRISPR mutants, has significantly enhanced our understanding of Dmc1's functions in meiosis and its potential implications in maintaining fertility, especially in the context of temperature-related effects on crops like wheat [1,4,6,7].
References：
1. Da Ines, Olivier, Bazile, Jeanne, Gallego, Maria E, White, Charles I. 2022. DMC1 attenuates RAD51-mediated recombination in Arabidopsis. In PLoS genetics, 18, e1010322. doi:10.1371/journal.pgen.1010322. https://pubmed.ncbi.nlm.nih.gov/36007010/
2. Ito, Masaru, Fujita, Yurika, Shinohara, Akira. 2023. Positive and negative regulators of RAD51/DMC1 in homologous recombination and DNA replication. In DNA repair, 134, 103613. doi:10.1016/j.dnarep.2023.103613. https://pubmed.ncbi.nlm.nih.gov/38142595/
3. San Filippo, Joseph, Sung, Patrick, Klein, Hannah. . Mechanism of eukaryotic homologous recombination. In Annual review of biochemistry, 77, 229-57. doi:10.1146/annurev.biochem.77.061306.125255. https://pubmed.ncbi.nlm.nih.gov/18275380/
4. Ito, Masaru, Furukohri, Asako, Matsuzaki, Kenichiro, Toyoda, Atsushi, Shinohara, Akira. 2023. FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination. In Nature communications, 14, 6857. doi:10.1038/s41467-023-42576-w. https://pubmed.ncbi.nlm.nih.gov/37891173/
5. Guo, Yu-Xuan, Yan, Shun-Ping, Wang, Ying-Xiang. . Recent advances in functional conservation and divergence of recombinase RAD51 and DMC1. In Yi chuan = Hereditas, 44, 398-413. doi:10.16288/j.yczz.22-016. https://pubmed.ncbi.nlm.nih.gov/35729697/
6. Draeger, Tracie N, Rey, María-Dolores, Hayta, Sadiye, Martin, Azahara C, Moore, Graham. 2023. DMC1 stabilizes crossovers at high and low temperatures during wheat meiosis. In Frontiers in plant science, 14, 1208285. doi:10.3389/fpls.2023.1208285. https://pubmed.ncbi.nlm.nih.gov/37615022/
7. Zainu, Akbar, Dupaigne, Pauline, Bouchouika, Soumya, Kumar, Rajeev, Baudat, Frédéric. 2024. FIGNL1-FIRRM is essential for meiotic recombination and prevents DNA damage-independent RAD51 and DMC1 loading. In Nature communications, 15, 7015. doi:10.1038/s41467-024-51458-8. https://pubmed.ncbi.nlm.nih.gov/39147779/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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