Ass1-KO Mouse
一般名
Ass1-KO
製品ID
S-KO-17507
背景情報
C57BL/6JCya
系統ID
KOCMP-11898-Ass1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Ass1-KO Mouse（カタログ番号S-KO-17507）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Ass1-KO
系統ID
KOCMP-11898-Ass1-B6J-VA
遺伝子名
製品ID
S-KO-17507
遺伝子別名
ASS, fold, Ass-1
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 2
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000102840
NCBIトランスクリプトID
NM_007494
ターゲット領域
Exon 8
有効領域の大きさ
~1.0 kb
遺伝子研究の概要
ASS1, also known as argininosuccinate synthetase 1, is a key enzyme in the urea cycle and the citrulline - nitric oxide cycle. It converts citrulline into argininosuccinate. This enzyme's function is crucial for maintaining nitrogen balance and is involved in various biological processes related to metabolism and immune response [1,5].
In macrophages, IFN - γ and/or LPS stimulation promotes STAT1 - mediated ASS1 transcription and JAK2 - mediated phosphorylation of ASS1 at tyrosine 87, leading to citrulline depletion, which is required for efficient proinflammatory signaling activation. Blockage of ASS1 - mediated citrulline depletion suppresses the host defense against bacterial infection in vivo, highlighting its role in innate immune - signaling [1].
In non - small cell lung cancer cells, loss of ASS1 increases sensitivity to erastin - induced ferroptosis both in vitro and in vivo, as ASS1 promotes reductive carboxylation of cytosolic glutamine and confers ferroptosis resistance [2].
In ovarian cancer, disruption of the ASS1 - related AMP/ATP balance activates AMPK and CPT1A, enhancing fatty acid oxidation (FAO) and anoikis resistance [3].
In colon cancer cells, prolonged loss of ASS1 promotes DNA damage, and following DNA damage, ASS1 is part of the p53 network that pauses cell cycle progression [4].
In triple - negative breast cancer, ASS1 binds to PHGDH and promotes its degradation to inhibit serine synthesis and tumorigenesis [6].
In the liver, down - regulation of ASS1 in the TAA - induced liver injury model is rescued by activation of FXR, which promotes ASS1 transcription and alleviates liver injury [7].
In conclusion, ASS1 is essential in multiple biological processes. Its role in immune response, cancer cell survival, and liver injury has been revealed through in vivo studies including gene - knockout (KO) or conditional - knockout (CKO) mouse models in relevant disease areas such as bacterial infection, cancer, and acute liver injury. These findings contribute to understanding the mechanisms of disease occurrence and may provide new therapeutic targets.
References：
1. Mao, Youxiang, Shi, Di, Li, Gen, Jiang, Peng. 2022. Citrulline depletion by ASS1 is required for proinflammatory macrophage activation and immune responses. In Molecular cell, 82, 527-541.e7. doi:10.1016/j.molcel.2021.12.006. https://pubmed.ncbi.nlm.nih.gov/35016033/
2. Hu, Qiangsheng, Dai, Jie, Zhang, Zheng, Zhang, Lele, Zhang, Peng. . ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells. In Cancer research, 83, 1646-1665. doi:10.1158/0008-5472.CAN-22-1999. https://pubmed.ncbi.nlm.nih.gov/36892426/
3. Feng, Xu, Ji, Zhaodong, Fan, Xiaoxi, Ren, Chunxia, Yang, Gong. 2024. ASS1 enhances anoikis resistance via AMPK/CPT1A-mediated fatty acid metabolism in ovarian cancer. In Cancer letters, , 217082. doi:10.1016/j.canlet.2024.217082. https://pubmed.ncbi.nlm.nih.gov/38914306/
4. Lim, Lisha Qiu Jin, Adler, Lital, Hajaj, Emma, Ulitsky, Igor, Erez, Ayelet. 2024. ASS1 metabolically contributes to the nuclear and cytosolic p53-mediated DNA damage response. In Nature metabolism, 6, 1294-1309. doi:10.1038/s42255-024-01060-5. https://pubmed.ncbi.nlm.nih.gov/38858597/
5. Diez-Fernandez, Carmen, Rüfenacht, Véronique, Häberle, Johannes. 2017. Mutations in the Human Argininosuccinate Synthetase (ASS1) Gene, Impact on Patients, Common Changes, and Structural Considerations. In Human mutation, 38, 471-484. doi:10.1002/humu.23184. https://pubmed.ncbi.nlm.nih.gov/28111830/
6. Luo, Wensong, Zou, Zizheng, Nie, Yuan, Zhou, Jiang, Luo, Zhiyong. 2024. ASS1 inhibits triple-negative breast cancer by regulating PHGDH stability and de novo serine synthesis. In Cell death & disease, 15, 319. doi:10.1038/s41419-024-06672-z. https://pubmed.ncbi.nlm.nih.gov/38710705/
7. Hong, Weilong, Peng, Xuyun, Zhou, Xue, Ye, Zhiqiang, Liang, Weicheng. 2022. FXR/ASS1 axis attenuates the TAA-induced liver injury through arginine metabolism. In Biochemical and biophysical research communications, 611, 31-37. doi:10.1016/j.bbrc.2022.04.073. https://pubmed.ncbi.nlm.nih.gov/35477090/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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グローバル由来:
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