Asap1-KO Mouse
一般名
Asap1-KO
製品ID
S-KO-17522
背景情報
C57BL/6JCya
系統ID
KOCMP-13196-Asap1-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Asap1-KO Mouse（カタログ番号S-KO-17522）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Asap1-KO
系統ID
KOCMP-13196-Asap1-B6J-VB
遺伝子名
製品ID
S-KO-17522
遺伝子別名
PAP, s19, DEF-1, Ddef1, mKIAA1249
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 15
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000177374
NCBIトランスクリプトID
NM_010026
ターゲット領域
Exon 4
有効領域の大きさ
~1.1 kb
遺伝子研究の概要
ASAP1, an ADP ribosylation factor (ARF) GTPase activation protein, has multiple functions including regulating cytoskeletal dynamics, small GTP - binding protein receptor recycling, and intracellular vesicle trafficking [5]. It is involved in pathways such as the IQGAP1/CDC42 pathway, which is related to tumor progression and chemotherapy resistance [1]. It also has a role in actin stress fiber organization via its N - BAR domain, contributing to cell migration, spreading, and focal adhesion dynamics [8].
In gastric cancer, ASAP1 upregulation promotes cell proliferation, migration, invasion, and chemotherapy resistance by inhibiting IQGAP1 ubiquitination and activating the CDC42 - EGFR - MAPK pathway [1]. In glioblastoma, EIF4A3 - induced circular RNA ASAP1 promotes tumorigenesis and temozolomide resistance via the NRAS/MEK1/ERK1 - 2 signaling pathway [2]. In invasive breast cancer, low ASAP1 expression is associated with worse recurrence - free survival, especially in ER - positive cases [3]. In hepatocellular carcinoma, high ASAP1 expression is linked to aggressive clinicopathological features and poor outcomes [4]. In zebrafish, Asap1 affects susceptibility to Mycobacterium by regulating macrophage migration [5]. In NSCLC, lncRNA ASAP1 - IT1 enhances cancer cell stemness via the miR - 509 - 3p/YAP1 axis [6]. In the MMTV - PyMT model of luminal breast cancer, loss of ASAP1 activates AKT, accelerates tumorigenesis, and promotes metastasis [7]. In extrahepatic cholangiocarcinoma, ASAP1 promotes tumor development by regulating the Wnt/β - catenin pathway [9]. In gastric cancer, high expression of ASAP1 is associated with depth of invasion, lymph node metastasis, pathological stage, and poor survival, and is positively associated with FAK expression [10].
In conclusion, ASAP1 plays crucial roles in multiple biological processes, especially in tumor - related events such as tumor progression, metastasis, and chemotherapy resistance across various cancer types. Studies using different models, including zebrafish and mouse models, have provided insights into its functions in disease conditions, highlighting its potential as a biomarker and therapeutic target.
References：
1. Xie, Wangkai, Han, Zheng, Zuo, Ziyi, Xue, Xiangyang, Lin, Kezhi. 2023. ASAP1 activates the IQGAP1/CDC42 pathway to promote tumor progression and chemotherapy resistance in gastric cancer. In Cell death & disease, 14, 124. doi:10.1038/s41419-023-05648-9. https://pubmed.ncbi.nlm.nih.gov/36792578/
2. Wei, Yutian, Lu, Chenfei, Zhou, Peng, Shi, ZhuMei, You, Yongping. . EIF4A3-induced circular RNA ASAP1 promotes tumorigenesis and temozolomide resistance of glioblastoma via NRAS/MEK1/ERK1-2 signaling. In Neuro-oncology, 23, 611-624. doi:10.1093/neuonc/noaa214. https://pubmed.ncbi.nlm.nih.gov/32926734/
3. Park, Hosub, Son, Hwangkyu, Cha, Hyebin, Chung, Min Sung, Paik, Seungsam. 2023. ASAP1 Expression in Invasive Breast Cancer and Its Prognostic Role. In International journal of molecular sciences, 24, . doi:10.3390/ijms241814355. https://pubmed.ncbi.nlm.nih.gov/37762658/
4. Bang, Seongsik, Jee, Seungyun, Son, Hwangkyu, Kim, Hyunsung, Paik, Seungsam. 2022. Clinicopathological Implications of ASAP1 Expression in Hepatocellular Carcinoma. In Pathology oncology research : POR, 28, 1610635. doi:10.3389/pore.2022.1610635. https://pubmed.ncbi.nlm.nih.gov/36110251/
5. Cui, Jia, Chen, Guangxin, Wen, Da, Zhao, Zhonghua, Wu, Changxin. 2020. Asap1 Affects the Susceptibility of Zebrafish to Mycobacterium by Regulating Macrophage Migration. In Frontiers in cellular and infection microbiology, 10, 519503. doi:10.3389/fcimb.2020.519503. https://pubmed.ncbi.nlm.nih.gov/33194781/
6. Liu, Yantao, Yang, Yuping, Zhang, Lingli, Chen, Kangwu, Zhao, Wei. 2021. LncRNA ASAP1-IT1 enhances cancer cell stemness via regulating miR-509-3p/YAP1 axis in NSCLC. In Cancer cell international, 21, 572. doi:10.1186/s12935-021-02270-7. https://pubmed.ncbi.nlm.nih.gov/34715859/
7. Schreiber, Caroline, Gruber, Annette, Roßwag, Sven, Garvalov, Boyan K, Sleeman, Jonathan P. 2022. Loss of ASAP1 in the MMTV-PyMT model of luminal breast cancer activates AKT, accelerates tumorigenesis, and promotes metastasis. In Cancer letters, 533, 215600. doi:10.1016/j.canlet.2022.215600. https://pubmed.ncbi.nlm.nih.gov/35181478/
8. Gasilina, Anjelika, Vitali, Teresa, Luo, Ruibai, Jian, Xiaoying, Randazzo, Paul A. 2019. The ArfGAP ASAP1 Controls Actin Stress Fiber Organization via Its N-BAR Domain. In iScience, 22, 166-180. doi:10.1016/j.isci.2019.11.015. https://pubmed.ncbi.nlm.nih.gov/31785555/
9. He, Jiaqi, Liu, Han, Cai, Jianhua, Wang, Jiwen, Liu, Houbao. 2024. ASAP1 promotes extrahepatic cholangiocarcinoma progression by regulating the Wnt/β-catenin pathway in vitro and in vivo. In American journal of cancer research, 14, 5178-5192. doi:10.62347/RKQX3504. https://pubmed.ncbi.nlm.nih.gov/39659924/
10. Luo, Qiong, Zhang, Suyun, Zhang, Donghuan, Chen, Xiangqi, Yang, Sheng. 2020. Expression of ASAP1 and FAK in gastric cancer and its clinicopathological significance. In Oncology letters, 20, 974-980. doi:10.3892/ol.2020.11612. https://pubmed.ncbi.nlm.nih.gov/32566028/
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精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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