Camkk1-KO Mouse
一般名
Camkk1-KO
製品ID
S-KO-17708
背景情報
C57BL/6JCya
系統ID
KOCMP-55984-Camkk1-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Camkk1-KO Mouse（カタログ番号S-KO-17708）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Camkk1-KO
系統ID
KOCMP-55984-Camkk1-B6J-VB
遺伝子名
製品ID
S-KO-17708
遺伝子別名
Camkk, caMKK 1, caM-KK 1, CaMKKalpha, caMKK alpha, caM-KK alpha
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000092937
NCBIトランスクリプトID
NM_018883.3
ターゲット領域
Exon 3~7
有効領域の大きさ
~2.2 kb
遺伝子研究の概要
Camkk1, also known as calcium/calmodulin-dependent protein kinase kinase 1, phosphorylates and enhances the catalytic activity of downstream kinases such as CaMKI, CaMKIV, and protein kinase B [4]. It is involved in regulating key physiological and pathological processes including tumorigenesis, neuronal morphogenesis, synaptic plasticity, transcription factor activation, and cellular energy homeostasis [4]. It also has a role in calcium signal transduction [3].
In non-alcoholic fatty liver disease (NAFLD), human umbilical cord mesenchymal stem cell-derived exosomes (MSC-ex) can transfer Camkk1, which ameliorates lipid accumulation in an AMP-activated protein kinase-dependent manner, inhibiting SREBP-1C-mediated fatty acid synthesis and enhancing peroxisome proliferator-activated receptor alpha (PPARα)-mediated fatty acid oxidation [1].
In lung cancer patients, the rs7214723 recessive CC genotype in the Camkk1 gene is associated with better overall survival, and the CRISPR/Cas9-guided single nucleotide editing shows that the T > C mutation in rs7214723 significantly inhibits cell proliferation and migration and promotes cell apoptosis [2].
In a cross-sectional study on cardiac patients, the rs7214723 polymorphism in Camkk1 was analyzed, and although no association was found between it and the risk of developing specific coronary artery disease (CAD) and aortic stenosis (AS), the results suggest its potential role in CVD susceptibility as a possible genetic biomarker [3].
In the context of Echinococcus granulosus-induced bone loss, Camkk1 is up-regulated after Nrf2 suppression. Silencing Camkk1 inhibits osteoclast differentiation, and the small molecule inhibitor Crenolani can alleviate bone loss in PSC-treated mice [5].
In conclusion, Camkk1 is crucial in multiple biological processes and disease conditions. Its role in lipid metabolism regulation in NAFLD, prognosis of lung cancer, potential association with cardiovascular diseases, and osteoclast differentiation in Echinococcus granulosus-induced bone loss has been revealed through various research models. Understanding Camkk1 contributes to a better understanding of these biological processes and disease mechanisms, potentially providing new directions for treatment strategies.
References：
1. Yang, Fuji, Wu, Yanshuang, Chen, Yifei, Jin, Jianhua, Yan, Yongmin. 2023. Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis. In JHEP reports : innovation in hepatology, 5, 100746. doi:10.1016/j.jhepr.2023.100746. https://pubmed.ncbi.nlm.nih.gov/37274776/
2. Zhang, Haorui, Chen, Bocen, Zou, Zixiu, Li, Feng, Wu, Junjie. 2021. Associations Between CAMKK1 Polymorphism rs7214723 and the Prognosis of Patients With Lung Cancer. In Frontiers in oncology, 11, 757484. doi:10.3389/fonc.2021.757484. https://pubmed.ncbi.nlm.nih.gov/34868969/
3. Beghi, Sofia, Cavaliere, Francesca, Manfredini, Matteo, Beghi, Cesare, Buschini, Annamaria. . Polymorphism rs7214723 in CAMKK1: a new genetic variant associated with cardiovascular diseases. In Bioscience reports, 41, . doi:10.1042/BSR20210326. https://pubmed.ncbi.nlm.nih.gov/34165505/
4. Petrvalska, Olivia, Honzejkova, Karolina, Koupilova, Nicola, Obsilova, Veronika, Obsil, Tomas. . 14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. In Protein science : a publication of the Protein Society, 32, e4805. doi:10.1002/pro.4805. https://pubmed.ncbi.nlm.nih.gov/37817008/
5. Huang, Yansheng, Huang, Yiping, Xiao, Jun, Ren, Qian, Wang, Sibo. 2024. Mechanisms of Nrf2 suppression and Camkk1 upregulation in Echinococcus granulosus-induced bone loss. In International journal of biological macromolecules, 288, 138521. doi:10.1016/j.ijbiomac.2024.138521. https://pubmed.ncbi.nlm.nih.gov/39674449/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
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