Hif1an-KO Mouse
一般名
Hif1an-KO
製品ID
S-KO-17905
背景情報
C57BL/6JCya
系統ID
KOCMP-319594-Hif1an-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Hif1an-KO Mouse（カタログ番号S-KO-17905）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Hif1an-KO
系統ID
KOCMP-319594-Hif1an-B6J-VA
遺伝子名
製品ID
S-KO-17905
遺伝子別名
FIH, FIH1, 2310046M24Rik, A830014H24Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 19
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000040455
NCBIトランスクリプトID
NM_176958
ターゲット領域
Exon 3
有効領域の大きさ
~1.6 kb
遺伝子研究の概要
Hif1an, also known as hypoxia-inducible factor 1-alpha subunit suppressor, plays a crucial role in regulating hypoxia-inducible factor 1-alpha (HIF-1α) stability and transcriptional action. It is part of the oxygen-sensing pathway, with the PHD family of oxygen-dependent prolyl hydroxylases and FIH1-mediated asparagine hydroxylation also involved in tuning HIF transcriptional activity [2]. Hif1an is important in various biological processes, and its inappropriate expression is associated with cancer development and immune control [1].
In breast cancer, low Hif1an expression accompanied by less immune infiltration is associated with poor prognosis. High Hif1an expression is linked to good overall survival, and its expression is closely related to chemokines and immune cell infiltration [1].
In keloid formation, circSLC8A1 regulates the miR-181a-5p/HIF1AN axis to restrain the biological functions of human keloid fibroblasts [3].
In cardiomyocytes, pinoresinol diglucoside ameliorates hypoxia/reperfusion-induced injury by regulating miR-142-3p and HIF1AN [4].
In choriocarcinoma, METTL3 promotes miR-21-5p maturation to accelerate choriocarcinoma progression via the HIF1AN-induced inactivation of the HIF1A/VEGF pathway [5].
In hypertrophic scar formation, Rynchopeterine inhibits the formation of hypertrophic scars by regulating the miR-21/HIF1AN axis [6].
In skin flap transplantation, M2 macrophage-derived exosomes induce angiogenesis and increase skin flap survival through HIF1AN/HIF-1α/VEGFA control [7].
In osteoblast differentiation, miR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells [8].
In conclusion, Hif1an is essential for regulating HIF-1α, which impacts multiple biological processes. Its dysregulation is involved in various diseases such as cancer, keloid, and hypertrophic scar formation, as well as in processes like angiogenesis and osteoblast differentiation. Studies on Hif1an contribute to understanding the underlying mechanisms of these diseases and biological processes, potentially providing new strategies for treatment and prevention.
References：
1. Tang, Shasha, Liu, Dongyang, Fang, Yuan, Wang, Hui, Cai, Fengfeng. 2023. Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer. In Frontiers in oncology, 13, 1080910. doi:10.3389/fonc.2023.1080910. https://pubmed.ncbi.nlm.nih.gov/36816977/
2. Kaelin, William G, Ratcliffe, Peter J. . Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. In Molecular cell, 30, 393-402. doi:10.1016/j.molcel.2008.04.009. https://pubmed.ncbi.nlm.nih.gov/18498744/
3. Yuan, Xiaoye, Chen, Baiye, Wang, Xueming. 2022. CircSLC8A1 targets miR-181a-5p/HIF1AN pathway to inhibit the growth, migration and extracellular matrix deposition of human keloid fibroblasts. In Burns : journal of the International Society for Burn Injuries, 49, 622-632. doi:10.1016/j.burns.2022.04.009. https://pubmed.ncbi.nlm.nih.gov/35610079/
4. Wei, Yuan, Xiao, Liang, Yingying, Liu, Haichen, Wang. 2022. Pinoresinol diglucoside ameliorates H/R-induced injury of cardiomyocytes by regulating miR-142-3p and HIF1AN. In Journal of biochemical and molecular toxicology, 36, e23175. doi:10.1002/jbt.23175. https://pubmed.ncbi.nlm.nih.gov/35962614/
5. Ye, Kefan, Li, Lingchuan, Wu, Bao, Wang, Dongjie. 2022. METTL3 m6A-dependently promotes miR-21-5p maturation to accelerate choriocarcinoma progression via the HIF1AN-induced inactivation of the HIF1A/VEGF pathway. In Genes & genomics, 44, 1311-1322. doi:10.1007/s13258-022-01309-x. https://pubmed.ncbi.nlm.nih.gov/36074324/
6. Zhao, Wenbin, Ye, Jianzhou, Yang, Xuesong, Zhang, Qiongyu, Li, Jiaqi. 2024. Rynchopeterine inhibits the formation of hypertrophic scars by regulating the miR-21/HIF1AN axis. In Experimental cell research, 440, 114114. doi:10.1016/j.yexcr.2024.114114. https://pubmed.ncbi.nlm.nih.gov/38823472/
7. Luo, Gaojie, Zhou, Zekun, Cao, Zheming, Tang, Juyu, Qing, Liming. 2023. M2 macrophage-derived exosomes induce angiogenesis and increase skin flap survival through HIF1AN/HIF-1α/VEGFA control. In Archives of biochemistry and biophysics, 751, 109822. doi:10.1016/j.abb.2023.109822. https://pubmed.ncbi.nlm.nih.gov/38030054/
8. Yin, Nuo, Zhu, Longzhang, Ding, Liang, Xue, Feng, Xiao, Haijun. 2019. MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells. In Cellular & molecular biology letters, 24, 51. doi:10.1186/s11658-019-0177-6. https://pubmed.ncbi.nlm.nih.gov/31410089/
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凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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