Vps29-KO Mouse
一般名
Vps29-KO
製品ID
S-KO-18044
背景情報
C57BL/6JCya
系統ID
KOCMP-56433-Vps29-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Vps29-KO Mouse（カタログ番号S-KO-18044）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Vps29-KO
系統ID
KOCMP-56433-Vps29-B6J-VB
遺伝子名
製品ID
S-KO-18044
遺伝子別名
PEP11, 2010015D08Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 5
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000155671
NCBIトランスクリプトID
NM_019780
ターゲット領域
Exon 2~4
有効領域の大きさ
~4.3 kb
遺伝子研究の概要
Vps29, short for vacuolar protein sorting 29, is a key component of the retromer complex. The retromer complex is involved in the endosome-to-Golgi retrieval cargo transport pathway, and Vps29 plays a crucial role in coordinating the physical assembly of retrieval complexes with regulatory components, thus being essential for endosomal recycling [1,4,5,6,8]. This process is vital for maintaining the proteostatic plasticity of the cell under variable physiological demands [1].
In Drosophila, Vps29 is dispensable for embryogenesis but required for retromer function in aging adults. Vps29 mutants show mislocalization of retromer from neuropil to soma with the Rab7 GTPase, leading to progressive endolysosomal dysfunction, impaired substrate clearance, and lysosomal stress, highlighting its role in brain homeostasis during aging [6]. In human lung cell lines, a loss-of-function genome-wide CRISPR screen showed that Vps29 is required for the infection of several coronaviruses including SARS-CoV-2 and ebolavirus, while it has an enhancing effect on influenza A virus infection. Deficiency in Vps29 causes changes in endosome morphology and acidity and attenuates endosomal protease activity, entrapping incoming coronavirus particles [2]. In a human iPSC-derived neuronal model of 4R tauopathy, a CRISPRi screen identified Vps29 as a genetic modifier of seeding-induced Tau propagation [3]. A bioinformatics and Mendelian randomization study indicated that Vps29 is overexpressed in hepatocellular carcinoma (HCC), is an independent prognostic factor, and is associated with an increased risk of HCC [7].
In conclusion, Vps29 is integral to endosomal recycling, which is essential for normal cellular function. Model-based research, such as in Drosophila and human cell lines, has revealed its significance in various disease-related processes, including viral infections, neurodegenerative diseases like tauopathy, and cancer, specifically HCC. Understanding Vps29's function through these models provides insights into potential therapeutic targets for these diseases.
References：
1. Baños-Mateos, Soledad, Rojas, Adriana L, Hierro, Aitor. 2019. VPS29, a tweak tool of endosomal recycling. In Current opinion in cell biology, 59, 81-87. doi:10.1016/j.ceb.2019.03.010. https://pubmed.ncbi.nlm.nih.gov/31051431/
2. Poston, Daniel, Weisblum, Yiska, Hobbs, Alvaro, Bieniasz, Paul D. 2022. VPS29 Exerts Opposing Effects on Endocytic Viral Entry. In mBio, 13, e0300221. doi:10.1128/mbio.03002-21. https://pubmed.ncbi.nlm.nih.gov/35229640/
3. Parra Bravo, Celeste, Giani, Alice Maria, Madero-Perez, Jesus, Gong, Shiaoching, Gan, Li. 2024. Human iPSC 4R tauopathy model uncovers modifiers of tau propagation. In Cell, 187, 2446-2464.e22. doi:10.1016/j.cell.2024.03.015. https://pubmed.ncbi.nlm.nih.gov/38582079/
4. Healy, Michael D, McNally, Kerrie E, Butkovič, Rebeka, Collins, Brett M, Cullen, Peter J. . Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome. In Cell, 186, 2219-2237.e29. doi:10.1016/j.cell.2023.04.003. https://pubmed.ncbi.nlm.nih.gov/37172566/
5. Fuse, Atsuhito, Furuya, Norihiko, Kakuta, Soichiro, Saiki, Shinji, Hattori, Nobutaka. 2015. VPS29-VPS35 intermediate of retromer is stable and may be involved in the retromer complex assembly process. In FEBS letters, 589, 1430-6. doi:10.1016/j.febslet.2015.04.040. https://pubmed.ncbi.nlm.nih.gov/25937119/
6. Ye, Hui, Ojelade, Shamsideen A, Li-Kroeger, David, Bellen, Hugo J, Shulman, Joshua M. 2020. Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brain. In eLife, 9, . doi:10.7554/eLife.51977. https://pubmed.ncbi.nlm.nih.gov/32286230/
7. Yao, Y-Q, Lv, J-L. . Potential functions and causal associations of VPS29 in hepatocellular carcinoma: a bioinformatic and Mendelian randomization study. In European review for medical and pharmacological sciences, 27, 9586-9600. doi:10.26355/eurrev_202310_34131. https://pubmed.ncbi.nlm.nih.gov/37916325/
8. Bonifacino, Juan S, Hurley, James H. 2008. Retromer. In Current opinion in cell biology, 20, 427-36. doi:10.1016/j.ceb.2008.03.009. https://pubmed.ncbi.nlm.nih.gov/18472259/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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