Ptdss1-KO Mouse

Ptdss1, encoding phosphatidylserine synthase 1, is an enzyme that catalyzes the conversion of phosphatidylcholine to phosphatidylserine, playing a key role in glycerophospholipid metabolism [1,3,5]. Glycerophospholipid metabolism is essential for cell membrane structure and function, and thus Ptdss1 is of great biological importance. Genetic models, such as transgenic zebrafish attempts to express wild-type and mutant Ptdss1, have been used to study its function, though transgene expression faced challenges [5].

In murine mammary tumors, depletion of Ptdss1 from tumor cells reduced ether-phosphatidylserine (ePS) levels, stunted tumor growth, and decreased tumor-associated macrophage (TAM) abundance [2]. In B cell lymphoma cell lines, inhibition of Ptdss1 caused an imbalance in the membrane phospholipidome, leading to B cell receptor hyperactivation and apoptotic cell death, and in a mouse xenograft model, it suppressed tumor growth [4]. These loss-of-function experiments in mouse models suggest Ptdss1's role in promoting tumor growth and maintaining a tumor-promoting microenvironment.

In conclusion, Ptdss1 is crucial for phosphatidylserine synthesis within the glycerophospholipid metabolism pathway. Model-based research, especially mouse models with Ptdss1 depletion, has revealed its significance in tumor-related processes, such as promoting tumor growth and modulating the tumor microenvironment, offering potential therapeutic targets for cancer treatment.