Dgkg-KO Mouse
一般名
Dgkg-KO
製品ID
S-KO-18550
背景情報
C57BL/6JCya
系統ID
KOCMP-110197-Dgkg-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Dgkg-KO Mouse（カタログ番号S-KO-18550）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Dgkg-KO
系統ID
KOCMP-110197-Dgkg-B6J-VA
遺伝子名
製品ID
S-KO-18550
遺伝子別名
90kDa, Dagk3, mKIAA4131, 2900055E17Rik, E430001K23Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 16
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000089925
NCBIトランスクリプトID
NM_138650
ターゲット領域
Exon 4~5
有効領域の大きさ
~1.5 kb
遺伝子研究の概要
Dgkg, encoding diacylglycerol kinase gamma, is a key enzyme in glycerophospholipid metabolism and phosphatidylinositol signaling pathways [7]. Diacylglycerol kinases are important regulators of cell signaling, and Dgkg may be involved in various biological processes [4].
In hepatocellular carcinoma (HCC), endothelial Dgkg promoted tumor angiogenesis and immunosuppressive regulatory T-cell differentiation. Hypoxia-induced HIF-1α activates Dgkg transcription, and upregulated Dgkg promotes HCC progression via the ZEB2/TGF-β1 axis. Targeting endothelial Dgkg enhanced the efficiency of dual blockade of PD-1 and VEGFR-2 in a mouse HCC model [1].
In Wagyu cattle, a homozygous missense variant in Dgkg was associated with hepatic fibrinogen storage disease [2].
In an epileptic patient, a heterozygous mutation in Dgkg was found, and an induced pluripotent stem cell line was generated for studying epilepsy mechanisms [3].
In colorectal cancer, Dgkg was hypermethylated, suppressing its expression. Ectopic expression of wild-type and mutant Dgkg suppressed cell migration, invasion, and Rac1 activity, suggesting a tumor-suppressor role [4].
In acute myeloid leukemia, high Dgkg expression was associated with a more favorable prognosis according to the TGCA database [5].
In cortical GABAergic interneurons, Dgkg was dominantly expressed in somatostatin-expressing neurons, and its functional study indicated an alteration in neurite outgrowth [6].
In glioblastoma, a hypoxia-induced alternative splicing transcript Dgkg-Δ exon13 promoted cellular proliferation, migration, and invasion [8].
In conclusion, Dgkg plays diverse roles in multiple biological processes and diseases. Its functions in tumor angiogenesis, immune evasion, and cell migration/invasion in cancers, as well as in neurological and metabolic-related diseases, have been revealed through various research models, including in vivo studies in mouse models. Understanding Dgkg provides insights into disease mechanisms and potential therapeutic targets.
References：
1. Zhang, Liren, Xu, Jiali, Zhou, Suiqing, Li, Qing, Wang, Xuehao. 2023. Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma. In Journal of hepatology, 80, 82-98. doi:10.1016/j.jhep.2023.10.006. https://pubmed.ncbi.nlm.nih.gov/37838036/
2. Jacinto, Joana G P, Wohlsein, Peter, Häfliger, Irene M, Grünberg, Walter, Drögemüller, Cord. 2023. A missense variant in DGKG as a recessive functional variant for hepatic fibrinogen storage disease in Wagyu cattle. In Journal of veterinary internal medicine, 37, 2631-2637. doi:10.1111/jvim.16865. https://pubmed.ncbi.nlm.nih.gov/37681469/
3. Liu, Ya-Qing, Ling, Tiao-Wen, Wang, Hong-Yan, Song, Wen-Jun, Wang, Tian-Cheng. 2022. Generation of an integration-free induced pluripotent stem cell line (LZUSHI001-A) from an epileptic patient with DGKG mutation. In Stem cell research, 61, 102768. doi:10.1016/j.scr.2022.102768. https://pubmed.ncbi.nlm.nih.gov/35421845/
4. Kai, Masahiro, Yamamoto, Eiichiro, Sato, Akiko, Toyota, Minoru, Suzuki, Hiromu. 2017. Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. In Molecular carcinogenesis, 56, 1743-1752. doi:10.1002/mc.22631. https://pubmed.ncbi.nlm.nih.gov/28218473/
5. Gravina, Teresa, Boggio, Chiara Maria Teresa, Gorla, Elisa, Corà, Davide, Baldanzi, Gianluca. 2023. Role of Diacylglycerol Kinases in Acute Myeloid Leukemia. In Biomedicines, 11, . doi:10.3390/biomedicines11071877. https://pubmed.ncbi.nlm.nih.gov/37509516/
6. Fukumoto, Keita, Tamada, Kota, Toya, Tsuyoshi, Yanagawa, Yuchio, Takumi, Toru. 2017. Identification of genes regulating GABAergic interneuron maturation. In Neuroscience research, 134, 18-29. doi:10.1016/j.neures.2017.11.010. https://pubmed.ncbi.nlm.nih.gov/29203264/
7. Taniguchi-Ponciano, Keiko, Hinojosa-Alvarez, Silvia, Hernandez-Perez, Jesus, Marrero-Rodriguez, Daniel, Mercado, Moises. 2024. Longitudinal multiomics analysis of aggressive pituitary neuroendocrine tumors: comparing primary and recurrent tumors from the same patient, reveals genomic stability and heterogeneous transcriptomic profiles with alterations in metabolic pathways. In Acta neuropathologica communications, 12, 142. doi:10.1186/s40478-024-01796-x. https://pubmed.ncbi.nlm.nih.gov/39217365/
8. Yang, Ming, Chu, Liangzhao, Lin, Shukai, Han, Feng, Liu, Jian. 2025. The alternatively spliced diacylglycerol kinase gamma-Δ exon13 transcript generated under hypoxia promotes glioblastoma progression. In Oncology research, 33, 1189-1198. doi:10.32604/or.2024.055102. https://pubmed.ncbi.nlm.nih.gov/40296900/
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