Capza1-KO Mouse
一般名
Capza1-KO
製品ID
S-KO-18742
背景情報
C57BL/6JCya
系統ID
KOCMP-12340-Capza1-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Capza1-KO Mouse（カタログ番号S-KO-18742）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Capza1-KO
系統ID
KOCMP-12340-Capza1-B6J-VB
遺伝子名
製品ID
S-KO-18742
遺伝子別名
CAPZ, CAZ1, Cappa1, capZ alpha-1
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 3
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000094028
NCBIトランスクリプトID
NM_009797
ターゲット領域
Exon 2
有効領域の大きさ
~1.2 kb
遺伝子研究の概要
Capza1, also known as capping actin protein of muscle Z-line alpha subunit 1, is a subunit of the capping protein complex CapZ, an actin-binding complex that can regulate actin cytoskeleton remodelling [3,6]. Actin filament assembly, which Capza1 is involved in, plays a crucial role in maintaining tumour cell morphology and movement, and thus is important in biological processes like epithelial-mesenchymal transition (EMT) [3].
In various cancers, Capza1 shows diverse functions. In lung adenocarcinoma (LUAD), high Capza1 expression has an unfavourable prognostic value, and it is associated with immune infiltration cells, immune checkpoints etc., suggesting it as a potential immunological antitumor target [1]. In gastric cancer, overexpression of Capza1 is associated with well-differentiated histology, smaller tumour size etc., and in vitro, forced expression of Capza1 decreases gastric cancer cell migration and invasion [7]. In hepatocellular carcinoma (HCC), Capza1 inhibits EMT by regulating actin cytoskeleton remodelling, reducing cell metastatic ability, and low expression of Capza1 promotes HCC cell invasion and migration both in vitro and in vivo [3,9]. Also, Helicobacter pylori-infected CAPZA1-overexpressing cells can give rise to cancer stem-cell marker CD44v9-positive cells, and CAPZA1 can inhibit autolysosome formation in H. pylori-infected gastric epithelial cells, increasing the risk of gastric carcinogenesis [2,4]. Moreover, polymorphisms in CAPZA1 may be associated with the development of gastric mucosal atrophy [5]. In addition, FAM21C can promote HCC invasion and metastasis by inhibiting the capping ability of CAPZA1 [6], and exosome-delivered circSTAU2 can inhibit gastric cancer progression by targeting the miR-589/CAPZA1 axis [8].
In conclusion, Capza1 plays a significant role in cancer-related biological processes, especially in tumour cell migration, invasion, and EMT, mainly through its function in regulating actin cytoskeleton remodelling. Studies on Capza1 in different cancer models help understand its role in cancer development, providing potential targets for cancer prognosis and treatment.
References：
1. Qin, Tingting, Xiang, Wanping, Mao, Yiming, Yang, Zhihao, Zhang, Hongpan. 2023. NcRNA-regulated CAPZA1 associated with prognostic and immunological effects across lung adenocarcinoma. In Frontiers in oncology, 12, 1025192. doi:10.3389/fonc.2022.1025192. https://pubmed.ncbi.nlm.nih.gov/36686785/
2. Tsugawa, Hitoshi, Mori, Hideki, Matsuzaki, Juntaro, Suematsu, Makoto, Suzuki, Hidekazu. 2018. CAPZA1 determines the risk of gastric carcinogenesis by inhibiting Helicobacter pylori CagA-degraded autophagy. In Autophagy, 15, 242-258. doi:10.1080/15548627.2018.1515530. https://pubmed.ncbi.nlm.nih.gov/30176157/
3. Huang, Deng, Cao, Li, Zheng, Shuguo. 2017. CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma. In Journal of experimental & clinical cancer research : CR, 36, 13. doi:10.1186/s13046-016-0474-0. https://pubmed.ncbi.nlm.nih.gov/28093067/
4. Tsugawa, Hitoshi, Kato, Chihiro, Mori, Hideki, Suematsu, Makoto, Suzuki, Hidekazu. 2019. Cancer Stem-Cell Marker CD44v9-Positive Cells Arise From Helicobacter pylori-Infected CAPZA1-Overexpressing Cells. In Cellular and molecular gastroenterology and hepatology, 8, 319-334. doi:10.1016/j.jcmgh.2019.05.008. https://pubmed.ncbi.nlm.nih.gov/31146068/
5. Yamaguchi, Naoyuki, Sakaguchi, Takuki, Isomoto, Hajime, Yashima, Kazuo, Tsukamoto, Kazuhiro. 2023. Polymorphism in autophagy-related genes LRP1 and CAPZA1 may promote gastric mucosal atrophy. In Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 45, 18. doi:10.1186/s41021-023-00274-5. https://pubmed.ncbi.nlm.nih.gov/37198664/
6. Lu, Yao, Huang, Deng, Wang, Baolin, Song, Juxian, Zheng, Shuguo. 2022. FAM21C Promotes Hepatocellular Carcinoma Invasion and Metastasis by Driving Actin Cytoskeleton Remodeling via Inhibiting Capping Ability of CAPZA1. In Frontiers in oncology, 11, 809195. doi:10.3389/fonc.2021.809195. https://pubmed.ncbi.nlm.nih.gov/35096613/
7. Lee, Young-Joon, Jeong, Sang-Ho, Hong, Soon-Chan, Yoo, Jiyun, Ko, Gyung Hyuck. 2013. Prognostic value of CAPZA1 overexpression in gastric cancer. In International journal of oncology, 42, 1569-77. doi:10.3892/ijo.2013.1867. https://pubmed.ncbi.nlm.nih.gov/23545944/
8. Zhang, Chenggang, Wei, Guanxin, Zhu, Xiuxian, Wu, Chuanqing, Tao, Kaixiong. 2023. Exosome-Delivered circSTAU2 Inhibits the Progression of Gastric Cancer by Targeting the miR-589/CAPZA1 Axis. In International journal of nanomedicine, 18, 127-142. doi:10.2147/IJN.S391872. https://pubmed.ncbi.nlm.nih.gov/36643863/
9. Huang, Deng, Cao, Li, Xiao, Le, Zheng, Ping, Zheng, Shu-Guo. 2019. Hypoxia induces actin cytoskeleton remodeling by regulating the binding of CAPZA1 to F-actin via PIP2 to drive EMT in hepatocellular carcinoma. In Cancer letters, 448, 117-127. doi:10.1016/j.canlet.2019.01.042. https://pubmed.ncbi.nlm.nih.gov/30742939/
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凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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