Gpat4-KO Mouse
一般名
Gpat4-KO
製品ID
S-KO-18766
背景情報
C57BL/6JCya
系統ID
KOCMP-102247-Gpat4-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Gpat4-KO Mouse（カタログ番号S-KO-18766）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Gpat4-KO
系統ID
KOCMP-102247-Gpat4-B6J-VA
遺伝子名
製品ID
S-KO-18766
遺伝子別名
Agpat6, Tsarg7
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 8
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000167004
NCBIトランスクリプトID
NM_018743
ターゲット領域
Exon 4
有効領域の大きさ
~0.9 kb
遺伝子研究の概要
Gpat4, glycerol-3-phosphate acyltransferase 4, is an endoplasmic reticulum membrane-anchored protein. It is crucial in glycerophospholipid biosynthesis and plays a part in lipid-related metabolic pathways. In plants, land-plant-specific GPATs, including those in the GPAT4/6/8 clade, are central to cutin and suberin monomer biosynthesis [1,3]. In mammals, it is involved in processes like adipogenesis and regulation of fatty acid metabolism [4,5]. Genetic models, such as knockout mice, have been valuable in studying its functions.
In Arabidopsis, the GPAT4/6/8 clade, along with the GPAT5/7 clade, is required for root suberization. GPAT4 has an additive effect with GPAT6 in root suberin biosynthesis and a redundant role with GPAT8 in wax production [1,3]. In mice, Gpat4 global and tissue-specific knockout models have shown that its deficiency in endocardial cells provokes ER stress response, enhances ER-mit communications, and triggers the cGAS-STING pathway, affecting heart development [2]. Gpat4-null pups had growth issues during suckling, and adult mice were protected from high-fat-diet-induced obesity. In brown adipocytes, Gpat4 limits oxidation of exogenous fatty acids [4]. Also, in 3T3-L1 adipocytes, shRNA-mediated knockdown of GPAT4 did not significantly decrease GPAT activity or inhibit lipid accumulation during adipocyte differentiation as much as GPAT3 knockdown, suggesting distinct roles for GPAT4 in adipogenesis [5]. In a high-fat-diet mouse model, hippocampal GPAT4 expression was up-regulated, and it may participate in diet-induced depression through the AMPK/CREB/BDNF pathway [6].
In conclusion, Gpat4 is essential for lipid-related biological processes in both plants and animals. In animals, studies using knockout mouse models have revealed its significance in heart development, obesity-related metabolism, adipogenesis, and the development of obesity-induced depression. These findings contribute to understanding the underlying mechanisms of related diseases and may provide potential targets for intervention.
References：
1. Gully, Kay, Berhin, Alice, De Bellis, Damien, Feussner, Ivo, Nawrath, Christiane. 2024. The GPAT4/6/8 clade functions in Arabidopsis root suberization nonredundantly with the GPAT5/7 clade required for suberin lamellae. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2314570121. doi:10.1073/pnas.2314570121. https://pubmed.ncbi.nlm.nih.gov/38739804/
2. Zhao, Tianyang, Jin, Kuipei, Wang, Xiaodong, Yang, Hongyuan, Yang, Zhongzhou. 2025. GPAT4 sustains endoplasmic reticulum homeostasis in endocardial cells and safeguards heart development. In Nature communications, 16, 3345. doi:10.1038/s41467-025-58722-5. https://pubmed.ncbi.nlm.nih.gov/40199910/
3. Zhang, Xuanhao, Gao, Huani, Liu, Yi, Zhao, Huayan, Lü, Shiyou. 2023. Function identification of Arabidopsis GPAT4 and GPAT8 in the biosynthesis of suberin and cuticular wax. In Plant science : an international journal of experimental plant biology, 339, 111933. doi:10.1016/j.plantsci.2023.111933. https://pubmed.ncbi.nlm.nih.gov/38036221/
4. Cooper, Daniel E, Grevengoed, Trisha J, Klett, Eric L, Coleman, Rosalind A. 2015. Glycerol-3-phosphate Acyltransferase Isoform-4 (GPAT4) Limits Oxidation of Exogenous Fatty Acids in Brown Adipocytes. In The Journal of biological chemistry, 290, 15112-20. doi:10.1074/jbc.M115.649970. https://pubmed.ncbi.nlm.nih.gov/25918168/
5. Shan, Dandan, Li, Jian-liang, Wu, Leeying, Gimeno, Ruth E, Cao, Jingsong. 2010. GPAT3 and GPAT4 are regulated by insulin-stimulated phosphorylation and play distinct roles in adipogenesis. In Journal of lipid research, 51, 1971-81. doi:10.1194/jlr.M006304. https://pubmed.ncbi.nlm.nih.gov/20181984/
6. Huang, Yin-Qiong, Wang, Yaofeng, Hu, Keyue, Lin, Shu, Lin, Xia-Hong. 2021. Hippocampal Glycerol-3-Phosphate Acyltransferases 4 and BDNF in the Progress of Obesity-Induced Depression. In Frontiers in endocrinology, 12, 667773. doi:10.3389/fendo.2021.667773. https://pubmed.ncbi.nlm.nih.gov/34054732/
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