Plscr1-KO Mouse
一般名
Plscr1-KO
製品ID
S-KO-18886
背景情報
C57BL/6JCya
系統ID
KOCMP-22038-Plscr1-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Plscr1-KO Mouse（カタログ番号S-KO-18886）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Plscr1-KO
系統ID
KOCMP-22038-Plscr1-B6J-VB
遺伝子名
製品ID
S-KO-18886
遺伝子別名
Nor1, Tra1, Tra1a, Tra1b, Tras1, Tras2, MmTRA1a, MmTRA1b
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 9
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000093801
NCBIトランスクリプトID
NM_011636
ターゲット領域
Exon 4
有効領域の大きさ
~1.4 kb
遺伝子研究の概要
Plscr1, short for phospholipid scramblase 1, is a protein-coding gene with significant functions in multiple biological processes. It is involved in maintaining phospholipid distribution in cell membranes, and its expression can be induced by interferons (IFNs) [1,3,5,6,8]. PLSCR1 plays a crucial role in the host innate immunity against viral infections, and it has also been linked to cell apoptosis, cancer progression, and cell differentiation [1,2,4,5,6,7,9]. Genetic models, such as gene knockout mouse models, are valuable tools to study its functions.
In terms of viral infections, genome-wide CRISPR-Cas9 screens have identified PLSCR1 as a cell-autonomous restriction factor against live SARS-CoV-2 infection in human lung epithelia and hepatocytes. IFNγ-induced PLSCR1 restricts not only SARS-CoV-2 USA-WA1/2020 but also the Delta and Omicron lineages, and it acts on other highly pathogenic coronaviruses [1]. It interferes with the uptake of SARS-CoV-2 in both endocytic and TMPRSS2-dependent fusion routes, directly targeting SARS-CoV-2-containing vesicles to prevent spike-mediated fusion and viral escape [1]. Moreover, PLSCR1-knockout 36T-3 cells show significantly higher levels of human cytomegalovirus (HCMV) plaque formation and replication compared to parental cells, indicating that PLSCR1 negatively regulates HCMV replication [5]. In influenza A virus infection, Plscr1-/-mice are more susceptible to H1N1 SIV infection, suggesting its antiviral function in vivo [4].
In conclusion, PLSCR1 is a multifunctional gene playing essential roles in antiviral immunity, as demonstrated by gene-knockout models in the context of SARS-CoV-2, HCMV, and influenza A virus infections. It also has implications in other disease-related processes such as glaucoma-associated apoptosis of retinal ganglion cells, and the progression of basal-like breast cancer and acute myeloid leukemia [2,7,9]. The study of PLSCR1 using knockout models helps to understand its biological functions and provides insights into potential therapeutic strategies for related diseases.
References：
1. Xu, Dijin, Jiang, Weiqian, Wu, Lizhen, Wilen, Craig B, MacMicking, John D. 2023. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. In Nature, 619, 819-827. doi:10.1038/s41586-023-06322-y. https://pubmed.ncbi.nlm.nih.gov/37438530/
2. Luo, Jingyi, Lian, Qing, Zhu, Deliang, Lu, Lin, Zhao, Ling. 2022. PLSCR1 promotes apoptosis and clearance of retinal ganglion cells in glaucoma pathogenesis. In Genes & diseases, 10, 1564-1581. doi:10.1016/j.gendis.2022.05.036. https://pubmed.ncbi.nlm.nih.gov/37397520/
3. Li, Minghua. . Fortifying immunity: PLSCR1 picks battle against SARS-CoV-2. In Cell host & microbe, 31, 1417-1419. doi:10.1016/j.chom.2023.08.014. https://pubmed.ncbi.nlm.nih.gov/37708846/
4. Liu, Yueyue, Lin, Shuqian, Xie, Yunhui, Xu, Zhigang, Wu, Jiaqiang. 2022. ILDR1 promotes influenza A virus replication through binding to PLSCR1. In Scientific reports, 12, 8515. doi:10.1038/s41598-022-12598-3. https://pubmed.ncbi.nlm.nih.gov/35595813/
5. Sadanari, Hidetaka, Takemoto, Masaya, Ishida, Tomoki, Murayama, Tsugiya, Kusano, Shuichi. 2022. The Interferon-Inducible Human PLSCR1 Protein Is a Restriction Factor of Human Cytomegalovirus. In Microbiology spectrum, 10, e0134221. doi:10.1128/spectrum.01342-21. https://pubmed.ncbi.nlm.nih.gov/35138119/
6. Ma, Ruiyi, Zhang, Xinyi, Li, Ruonan, Lei, Xiaobo, Wang, Jianwei. 2025. PLSCR1 suppresses SARS-CoV-2 infection by downregulating cell surface ACE2. In Journal of virology, 99, e0208524. doi:10.1128/jvi.02085-24. https://pubmed.ncbi.nlm.nih.gov/39945535/
7. Huang, Panpan, Liao, Ruocen, Chen, Xingyu, Cao, Qianhua, Dong, Chenfang. 2020. Nuclear translocation of PLSCR1 activates STAT1 signaling in basal-like breast cancer. In Theranostics, 10, 4644-4658. doi:10.7150/thno.43150. https://pubmed.ncbi.nlm.nih.gov/32292520/
8. Le Pen, Jérémie, Paniccia, Gabrielle, Kinast, Volker, MacDonald, Margaret R, Rice, Charles M. 2024. A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist. In PLoS biology, 22, e3002767. doi:10.1371/journal.pbio.3002767. https://pubmed.ncbi.nlm.nih.gov/39316623/
9. Li, Hui, Xu, Jingyan, Zhou, Yuxin, Guo, Qinglong, Hui, Hui. 2017. PLSCR1/IP3R1/Ca2+ axis contributes to differentiation of primary AML cells induced by wogonoside. In Cell death & disease, 8, e2768. doi:10.1038/cddis.2017.175. https://pubmed.ncbi.nlm.nih.gov/28492556/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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