Tspan5-KO Mouse
一般名
Tspan5-KO
製品ID
S-KO-19010
背景情報
C57BL/6JCya
系統ID
KOCMP-56224-Tspan5-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Tspan5-KO Mouse（カタログ番号S-KO-19010）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Tspan5-KO
系統ID
KOCMP-56224-Tspan5-B6J-VB
遺伝子名
製品ID
S-KO-19010
遺伝子別名
NET-4, Tm4sf9, 2810455A09Rik, 4930505M03Rik
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 3
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000029800
NCBIトランスクリプトID
NM_019571
ターゲット領域
Exon 4~7
有効領域の大きさ
~4.9 kb
遺伝子研究の概要
Tspan5, a member of the tetraspanin family, is involved in multiple biological functions. It can interact with specific partner proteins in tetraspanin-enriched microdomains, regulating their subcellular localization and function. It is associated with pathways such as Notch signaling, and plays important roles in processes like cell fusion, epithelial-mesenchymal transition (EMT), and synaptic function, with implications for various diseases [1,2,4].
In placental trophoblastic cells, Tspan5 promotes the EMT process to regulate syncytialization by activating Notch signalling. Knockdown of Tspan5 inhibits cell fusion and EMT-related protein levels [1].
In hepatocellular carcinoma (HCC), Tspan5 promotes EMT and tumour metastasis by activating Notch signalling, and its high expression is associated with poor prognosis. Lentivirus-mediated alteration of Tspan5 expression in HCC cells shows its role in promoting cell migration and tumour metastasis in vitro and in vivo [2].
In alcohol-related disorders, ethanol and acamprosate treatment can down-regulate Tspan5 expression, influencing serotonin and kynurenine concentrations [3].
In the nervous system, Tspan5 knockdown in mouse pyramidal excitatory neurons causes a decrease in spine number due to spine maturation defects, and it promotes neuroligin-1 surface clustering [4]. In rat hippocampal neurons, Tspan5 promotes AMPA receptor exocytosis by interacting with the AP4 complex [5].
In gastric cancer, Tspan5 is down-regulated, and its up-regulation inhibits cell proliferation and tumour growth by modulating cell cycle-related proteins [6].
In HCC, depletion of Tspan5 induces oncogene-induced senescence through the p16INK4a/pRb pathways and affects actin polymerization and MRTF-A-FLNA complex formation [7].
In conclusion, Tspan5 is crucial in multiple biological processes and diseases. Its role in cell fusion, EMT, synaptic function, and tumour-related processes has been revealed through various functional studies including knockdown experiments. These findings contribute to understanding normal development and disease mechanisms, such as in placental development, cancer metastasis, and alcohol-related disorders, providing potential therapeutic targets.
References：
1. Tang, Hai-Yu, Lin, Mei, Liang, Yong-Qian, Yi, Hong-Gan, Yang, Man. 2023. Tspan5 promotes the EMT process to regulate the syncytialization of trophoblast cells by activating Notch signalling. In Zygote (Cambridge, England), 31, 498-506. doi:10.1017/S0967199423000369. https://pubmed.ncbi.nlm.nih.gov/37485669/
2. Xie, Qian, Guo, Huiling, He, Peirong, Wu, Yingsong, Li, Ji-Liang. 2021. Tspan5 promotes epithelial-mesenchymal transition and tumour metastasis of hepatocellular carcinoma by activating Notch signalling. In Molecular oncology, 15, 3184-3202. doi:10.1002/1878-0261.12980. https://pubmed.ncbi.nlm.nih.gov/33955149/
3. Ho, Ming-Fen, Zhang, Cheng, Zhang, Lingxin, Li, Hu, Weinshilboum, Richard. 2020. TSPAN5 influences serotonin and kynurenine: pharmacogenomic mechanisms related to alcohol use disorder and acamprosate treatment response. In Molecular psychiatry, 26, 3122-3133. doi:10.1038/s41380-020-0855-9. https://pubmed.ncbi.nlm.nih.gov/32753686/
4. Moretto, Edoardo, Longatti, Anna, Murru, Luca, Thoumine, Olivier, Passafaro, Maria. . TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. In Cell reports, 29, 1130-1146.e8. doi:10.1016/j.celrep.2019.09.051. https://pubmed.ncbi.nlm.nih.gov/31665629/
5. Moretto, Edoardo, Miozzo, Federico, Longatti, Anna, Cingolani, Lorenzo A, Passafaro, Maria. 2023. The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex. In eLife, 12, . doi:10.7554/eLife.76425. https://pubmed.ncbi.nlm.nih.gov/36795458/
6. He, Peirong, Wang, Suihai, Zhang, Xuefeng, Jiang, Bo, Li, Ji-Liang. . Tspan5 is an independent favourable prognostic factor and suppresses tumour growth in gastric cancer. In Oncotarget, 7, 40160-40173. doi:10.18632/oncotarget.9514. https://pubmed.ncbi.nlm.nih.gov/27223087/
7. Schreyer, Laura, Mittermeier, Constanze, Franz, Miriam J, Aigner, Achim, Muehlich, Susanne. 2021. Tetraspanin 5 (TSPAN5), a Novel Gatekeeper of the Tumor Suppressor DLC1 and Myocardin-Related Transcription Factors (MRTFs), Controls HCC Growth and Senescence. In Cancers, 13, . doi:10.3390/cancers13215373. https://pubmed.ncbi.nlm.nih.gov/34771537/
品質管理基準
精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
環境基準:
SPF対応地域:
グローバル由来:
Cyagenお問い合わせ
カスタムの動物モデルに関するご相談は、下記のフォームにご記入いただき、ご連絡いただくか見積もりをご依頼ください。
Cyagenはお客様のプライバシーを大変重視しています。当社の最新の製品や情報をお届けしたいと思っています。お客様の設定をご確認ください。
これらの配信はいつでも解除できます。配信停止方法およびデータ保護の詳細は プライバシーポリシー をご確認ください。
以下のボタンをクリックすることで、このフォームにご入力いただいた個人情報をCyagenが保存・処理し、ご要望のコンテンツを提供することに同意されたことになります。