Npr1-KO Mouse
一般名
Npr1-KO
製品ID
S-KO-19064
背景情報
C57BL/6JCya
系統ID
KOCMP-18160-Npr1-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Npr1-KO Mouse（カタログ番号S-KO-19064）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Npr1-KO
系統ID
KOCMP-18160-Npr1-B6J-VA
遺伝子名
製品ID
S-KO-19064
遺伝子別名
GC-A, NPR-A, NPRA, Pndr
遺伝子別名
C57BL/6JCya
遺伝子正式名称
natriuretic peptide receptor 1
NCBI ID
修正
Conventional knockout
染色体
Chr 3
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000029540
NCBIトランスクリプトID
NM_008727
ターゲット領域
Exon 2~7
有効領域の大きさ
~4.0 kb
遺伝子研究の概要
Npr1, also known as Nonexpressor of pathogenesis-related genes 1, is a master regulator in plants. It is central to the salicylic acid (SA)-mediated systemic acquired resistance (SAR), a broad-spectrum disease resistance mechanism. NPR1 controls about 90% of SA-dependent transcriptome in Arabidopsis and plays a crucial role in plant survival under biotic and abiotic stresses [2,6].
NPR1 promotes cell survival during the plant immune response by forming salicylic acid-induced NPR1 condensates (SINCs). These SINCs are enriched with stress-response proteins. The transition of NPR1 into condensates is required for the formation of the NPR1-Cullin 3 E3 ligase complex, which ubiquitinates SINC-localized substrates like EDS1 and specific WRKY transcription factors [1]. Also, NPR1 is a SA receptor. SA-bound NPR1 dimer activates transcription by bridging two TGA transcription factor dimers to form an enhanceosome. Moreover, NPR1 functions in plant immunity through forming distinct nuclear and cytoplasmic biomolecular condensates [2,3]. In addition, both NPR1 and NPR3/NPR4 are SA receptors, but they play opposite roles in transcriptional regulation of SA-induced defense gene expression [4]. The perception of SA by NPR1 and NPR4 is needed for the activation of N-hydroxypipecolic acid biosynthesis, which is essential for inducing systemic acquired resistance [5]. High air humidity dampens the SA pathway and NPR1 function, as NPR1 is less ubiquitinated and has lower promoter binding affinity under high humidity [7]. A fungal effector CfEC12 can target the plant SA defense pathway by suppressing the interaction between MdNIMIN2 and MdNPR1, thus promoting fungal infection [8]. Overexpressing NPR1 in transgenic plants can enhance resistance to biotic and abiotic stresses, but may also cause detrimental morphological traits [9].
In summary, NPR1 is a key regulator in plant immunity, mainly through its functions in SA-mediated signaling, transcriptional regulation, and formation of condensates. Understanding NPR1's role helps in developing strategies to engineer plant resistance against biotic and abiotic stresses.
References：
1. Zavaliev, Raul, Mohan, Rajinikanth, Chen, Tianyuan, Dong, Xinnian. 2020. Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response. In Cell, 182, 1093-1108.e18. doi:10.1016/j.cell.2020.07.016. https://pubmed.ncbi.nlm.nih.gov/32810437/
2. Zavaliev, Raul, Dong, Xinnian. 2023. NPR1, a key immune regulator for plant survival under biotic and abiotic stresses. In Molecular cell, 84, 131-141. doi:10.1016/j.molcel.2023.11.018. https://pubmed.ncbi.nlm.nih.gov/38103555/
3. Kumar, Shivesh, Zavaliev, Raul, Wu, Qinglin, Dong, Xinnian, Zhou, Pei. 2022. Structural basis of NPR1 in activating plant immunity. In Nature, 605, 561-566. doi:10.1038/s41586-022-04699-w. https://pubmed.ncbi.nlm.nih.gov/35545668/
4. Ding, Yuli, Sun, Tongjun, Ao, Kevin, Li, Xin, Zhang, Yuelin. 2018. Opposite Roles of Salicylic Acid Receptors NPR1 and NPR3/NPR4 in Transcriptional Regulation of Plant Immunity. In Cell, 173, 1454-1467.e15. doi:10.1016/j.cell.2018.03.044. https://pubmed.ncbi.nlm.nih.gov/29656896/
5. Liu, Yanan, Sun, Tongjun, Sun, Yulin, Li, Xin, Zhang, Yuelin. 2020. Diverse Roles of the Salicylic Acid Receptors NPR1 and NPR3/NPR4 in Plant Immunity. In The Plant cell, 32, 4002-4016. doi:10.1105/tpc.20.00499. https://pubmed.ncbi.nlm.nih.gov/33037144/
6. Sun, Yali, Detchemendy, Thomas Ward, Pajerowska-Mukhtar, Karolina Marta, Mukhtar, M Shahid. 2018. NPR1 in JazzSet with Pathogen Effectors. In Trends in plant science, 23, 469-472. doi:10.1016/j.tplants.2018.04.007. https://pubmed.ncbi.nlm.nih.gov/29753632/
7. Yao, Lingya, Jiang, Zeyu, Wang, Yiping, Wan, Shiwei, Xin, Xiu-Fang. 2023. High air humidity dampens salicylic acid pathway and NPR1 function to promote plant disease. In The EMBO journal, 42, e113499. doi:10.15252/embj.2023113499. https://pubmed.ncbi.nlm.nih.gov/37728254/
8. Shang, Shengping, Liu, Guangli, Zhang, Song, Zhang, Rong, Sun, Guangyu. 2023. A fungal CFEM-containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity. In Plant biotechnology journal, 22, 82-97. doi:10.1111/pbi.14166. https://pubmed.ncbi.nlm.nih.gov/37596985/
9. Silva, Katchen Julliany P, Mahna, Nasser, Mou, Zhonglin, Folta, Kevin M. 2018. NPR1 as a transgenic crop protection strategy in horticultural species. In Horticulture research, 5, 15. doi:10.1038/s41438-018-0026-1. https://pubmed.ncbi.nlm.nih.gov/29581883/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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