Morrbid-KO Mouse
一般名
Morrbid-KO
製品ID
S-KO-20377
背景情報
C57BL/6JCya
系統ID
KOCMP-100043424-Morrbid-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Morrbid-KO Mouse（カタログ番号S-KO-20377）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Morrbid-KO
系統ID
KOCMP-100043424-Morrbid-B6J-VA
遺伝子名
製品ID
S-KO-20377
遺伝子別名
Cytor, Gm14005, Mir4435-2hg
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 2
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000225087
NCBIトランスクリプトID
--
ターゲット領域
Exon 1~2
有効領域の大きさ
~181.9 kb
遺伝子研究の概要
Morrbid, also known as Myeloid RNA regulator of Bim-induced death, is a long noncoding RNA (lncRNA) that has emerged as a significant regulator in multiple biological processes. It is involved in pathways related to apoptosis, cell differentiation, and splicing regulation, thereby playing a crucial role in maintaining normal cellular functions and influencing disease development [1,2,3,4,5,6,8]. Genetic models, such as knockout (KO) mouse models, have been instrumental in uncovering its functions.
In the context of acute myocardial infarction (AMI), Morrbid expression is increased in cardiomyocytes under hypoxia or oxidative stress and in mouse hearts with AMI. Overexpression of Morrbid reduces infarct size and cardiac dysfunction, while cardiomyocyte-specific Morrbid-KO (Morrbidfl/fl/Myh6-Cre) mice show deteriorated infarct size and cardiac function. This indicates that Morrbid protects hearts from AMI via anti-apoptosis through its target gene serpine1 [1].
In the case of monocyte-macrophage differentiation and atherogenesis, Morrbid expression increases during differentiation. Morrbid knockdown inhibits this differentiation and macrophage activity, and Morrbid-overexpressing mice show enhanced monocytes/macrophages recruitment and atherosclerotic lesion formation, while monocyte/macrophage-specific Morrbid knockout has the opposite effect, suggesting its role in atherogenesis [2].
In the context of leukemia, loss of Morrbid in mouse models of juvenile myelomonocytic leukemia (JMML) driven by the Shp2E76K/+ mutation and in models of hyperglycemia-induced leukemia corrects myeloid and erythroid cell abnormalities, prolongs survival, and mitigates the disease, indicating Morrbid's contribution to leukemia pathogenesis [3,7].
In conclusion, Morrbid is a key lncRNA that regulates apoptosis, cell differentiation, and splicing, influencing diseases such as AMI, atherosclerosis, and leukemia. The use of Morrbid KO/CKO mouse models has provided valuable insights into its role in these disease areas, highlighting its potential as a therapeutic target for ischemic heart diseases and myeloid neoplasms [1,2,3,7].
References：
1. Yu, Yang, Yang, Haiqiong, Li, Qiuting, Wang, Xiaobin, Zhang, Chunxiang. 2023. Stress-enhanced cardiac lncRNA Morrbid protects hearts from acute myocardial infarction. In JCI insight, 8, . doi:10.1172/jci.insight.165568. https://pubmed.ncbi.nlm.nih.gov/37432746/
2. Xiang, Di, Jiang, Lei, Yuan, Qiong, Qin, Gangjian, Zhang, Chunxiang. 2023. Leukocyte-Specific Morrbid Promotes Leukocyte Differentiation and Atherogenesis. In Research (Washington, D.C.), 6, 0187. doi:10.34133/research.0187. https://pubmed.ncbi.nlm.nih.gov/37426471/
3. Cai, Zhigang, Zhang, Chi, Kotzin, Jonathan J, Henao-Mejia, Jorge, Kapur, Reuben. . Role of lncRNA Morrbid in PTPN11(Shp2)E76K-driven juvenile myelomonocytic leukemia. In Blood advances, 4, 3246-3251. doi:10.1182/bloodadvances.2020002123. https://pubmed.ncbi.nlm.nih.gov/32697817/
4. Cai, Zhigang, Aguilera, Fabiola, Ramdas, Baskar, Henao-Mejia, Jorge, Kapur, Reuben. . Targeting Bim via a lncRNA Morrbid Regulates the Survival of Preleukemic and Leukemic Cells. In Cell reports, 31, 107816. doi:10.1016/j.celrep.2020.107816. https://pubmed.ncbi.nlm.nih.gov/32579941/
5. Kotzin, Jonathan J, Iseka, Fany, Wright, Jasmine, Wherry, E John, Henao-Mejia, Jorge. 2019. The long noncoding RNA Morrbid regulates CD8 T cells in response to viral infection. In Proceedings of the National Academy of Sciences of the United States of America, 116, 11916-11925. doi:10.1073/pnas.1819457116. https://pubmed.ncbi.nlm.nih.gov/31138702/
6. Fefilova, Anna, Melnikov, Pavel, Prikazchikova, Tatiana, Sergeeva, Olga, Zatsepin, Timofei S. 2020. Murine Long Noncoding RNA Morrbid Contributes in the Regulation of NRAS Splicing in Hepatocytes In Vitro. In International journal of molecular sciences, 21, . doi:10.3390/ijms21165605. https://pubmed.ncbi.nlm.nih.gov/32764370/
7. Cai, Zhigang, Lu, Xiaoyu, Zhang, Chi, Haneline, Laura, Kapur, Reuben. . Hyperglycemia cooperates with Tet2 heterozygosity to induce leukemia driven by proinflammatory cytokine-induced lncRNA Morrbid. In The Journal of clinical investigation, 131, . doi:10.1172/JCI140707. https://pubmed.ncbi.nlm.nih.gov/33090974/
8. Kotzin, Jonathan J, Spencer, Sean P, McCright, Sam J, Flavell, Richard A, Henao-Mejia, Jorge. 2016. The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan. In Nature, 537, 239-243. doi:10.1038/nature19346. https://pubmed.ncbi.nlm.nih.gov/27525555/
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凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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