Map2k6-KO Mouse
一般名
Map2k6-KO
製品ID
S-KO-20380
背景情報
C57BL/6JCya
系統ID
KOCMP-26399-Map2k6-B6J-VB
状況
このマウス系統を論文で使用する場合は、「Map2k6-KO Mouse（カタログ番号S-KO-20380）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Map2k6-KO
系統ID
KOCMP-26399-Map2k6-B6J-VB
遺伝子名
製品ID
S-KO-20380
遺伝子別名
MEK6, MKK6, MAPKK6, Prkmk6, SAPKK3, MAPKK 6
遺伝子別名
C57BL/6JCya
NCBI ID
修正
Conventional knockout
染色体
Chr 11
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000020949
NCBIトランスクリプトID
NM_011943
ターゲット領域
Exon 3~4
有効領域の大きさ
~1.4 kb
遺伝子研究の概要
Map2k6, also known as Mitogen-activated protein kinase kinase 6 (Mkk6), is a key component in the mitogen-activated protein kinase (MAPK) signaling pathway. It is involved in regulating various biological processes such as chromatin remodeling, cell proliferation, apoptosis, and stress responses. Dysregulation of Map2k6 has been linked to several diseases, highlighting its biological importance [1-10].
In somatic cell reprogramming, Map2k6 acts as a chromatin relaxer, enhancing the process. Its function in heterochromatin loosening and reprogramming requires kinase activity but is independent of its well-known target P38. It phosphorylates Gatad2b, which in turn elevates histone acetylation levels and loosens heterochromatin, promoting pluripotency gene expression [1].
In polycystic ovary syndrome (PCOS), circASPH promotes KGN cells proliferation through the miR-375/MAP2K6 axis, and circ_0005925 promotes granulosa cell growth by targeting miR-324-3p to upregulate MAP2K6, suggesting its role in PCOS-related cell growth regulation [2,6].
In ovarian cancer, MAP2K6-FP enhances the sensitiveness of paclitaxel via inducing autophagy [3].
In nasopharyngeal carcinoma, high MAP2K6 expression is associated with radiation resistance and poor prognosis [4].
In LPS-induced C28/I2 chondrocyte injury, circRNA-MSR regulates the process through the miR-643/MAP2K6 signaling pathway [5].
In hepatocellular carcinoma, APEX1 promotes oncogenicity via regulation of MAP2K6 [7].
In gut stem cell aging, mTORC1 increases MKK6 protein synthesis, activating the p38 MAPK-p53 pathway, leading to stem cell and villus aging [8].
In cell senescence, lncRNA MAGI2-AS3 affects the process via the ROS/MAP2K6/p38 signaling pathway [9].
In conclusion, Map2k6 plays essential roles in diverse biological processes and is closely associated with multiple diseases. Functional studies, especially those using in vivo models like KO/CKO mouse models (although not explicitly mentioned in the provided abstracts but commonly used in such research), could further clarify its detailed mechanisms in these biological processes and disease conditions, providing potential therapeutic targets for related diseases.
References：
1. Xing, Guangsuo, Liu, Zichao, Huang, Luyuan, Liu, Xingguo, Chen, Keshi. 2021. MAP2K6 remodels chromatin and facilitates reprogramming by activating Gatad2b-phosphorylation dependent heterochromatin loosening. In Cell death and differentiation, 29, 1042-1054. doi:10.1038/s41418-021-00902-z. https://pubmed.ncbi.nlm.nih.gov/34815549/
2. Wu, Gengxiang, Xia, Jing, Yang, Zhe, Yin, Tailang, Yang, Jing. 2020. CircASPH promotes KGN cells proliferation through miR-375/MAP2K6 axis in Polycystic Ovary Syndrome. In Journal of cellular and molecular medicine, 26, 1817-1825. doi:10.1111/jcmm.16231. https://pubmed.ncbi.nlm.nih.gov/33372369/
3. Guo, Ying, Yuan, Jin, Yin, Shuqin, Shuai, Rong, Kang, Jiali. . MAP2K6-FP Enhances the Sensitiveness of Paclitaxel for Ovarian Cancer via Inducing Autophagy. In International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 27, 1082-1087. doi:10.1097/IGC.0000000000001003. https://pubmed.ncbi.nlm.nih.gov/28604448/
4. Li, Zhanzhan, Li, Na, Shen, Liangfang. 2018. MAP2K6 is associated with radiation resistance and adverse prognosis for locally advanced nasopharyngeal carcinoma patients. In Cancer management and research, 10, 6905-6912. doi:10.2147/CMAR.S184689. https://pubmed.ncbi.nlm.nih.gov/30588096/
5. Jia, Zhen, Wei, Qing-Jun. 2021. CircRNA-MSR Regulates LPS-Induced C28/I2 Chondrocyte Injury through miR-643/MAP2K6 Signaling Pathway. In Cartilage, 13, 785S-795S. doi:10.1177/19476035211044826. https://pubmed.ncbi.nlm.nih.gov/34581623/
6. Tu, Peng, Yan, Sujuan, Zhang, Fengping. 2022. Circ_0005925 Promotes Granulosa Cell Growth by Targeting MiR-324-3p to Upregulate MAP2K6 in Polycystic Ovary Syndrome. In Biochemical genetics, 61, 21-34. doi:10.1007/s10528-022-10238-z. https://pubmed.ncbi.nlm.nih.gov/35689709/
7. Sun, Zhipeng, Chen, Guangyang, Wang, Liang, Xu, Guangzhong, Zhang, Nengwei. 2022. APEX1 promotes the oncogenicity of hepatocellular carcinoma via regulation of MAP2K6. In Aging, 14, 7959-7971. doi:10.18632/aging.204325. https://pubmed.ncbi.nlm.nih.gov/36205565/
8. He, Dan, Wu, Hongguang, Xiang, Jinnan, Liu, Huijuan, Li, Baojie. 2020. Gut stem cell aging is driven by mTORC1 via a p38 MAPK-p53 pathway. In Nature communications, 11, 37. doi:10.1038/s41467-019-13911-x. https://pubmed.ncbi.nlm.nih.gov/31896747/
9. Zhang, Yingmin, Qiao, Xinhua, Liu, Lihui, Chen, Runsheng, Chen, Chang. 2022. Long noncoding RNA MAGI2-AS3 regulates the H2O2 level and cell senescence via HSPA8. In Redox biology, 54, 102383. doi:10.1016/j.redox.2022.102383. https://pubmed.ncbi.nlm.nih.gov/35797800/
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