Mgp-KO Mouse
一般名
Mgp-KO
製品ID
S-KO-20398
背景情報
C57BL/6JCya
系統ID
KOCMP-17313-Mgp-B6J-VA
状況
このマウス系統を論文で使用する場合は、「Mgp-KO Mouse（カタログ番号S-KO-20398）はサイアジェンから購入しました。」と引用してください。
製品タイプ
年齢
遺伝子型
性別
数量
標準的な配送方法では、少なくとも3匹のヘテロ接合体キャリアを保証しています。ホモ接合体キャリアや指定された性別の個体の繁殖サービスも利用可能です。
基本情報
系統名
Mgp-KO
系統ID
KOCMP-17313-Mgp-B6J-VA
遺伝子名
製品ID
S-KO-20398
遺伝子別名
Mglap
遺伝子別名
C57BL/6JCya
遺伝子正式名称
matrix Gla protein
NCBI ID
修正
Conventional knockout
染色体
Chr 6
表現型
アプリケーション
--
さらに
系統詳細
EnsemblトランスクリプトID
ENSMUST00000032342
NCBIトランスクリプトID
NM_008597
ターゲット領域
Exon 2~4
有効領域の大きさ
~2.8 kb
遺伝子研究の概要
Mgp, also known as Matrix Gla protein, is a vitamin K-dependent post-translationally modified protein. It is a potent inhibitor of extracellular matrix mineralization, playing a key role in preventing ectopic calcification in tissues like cartilage and the vascular system. Mutations in the Mgp gene cause Keutel syndrome, characterized by abnormal cartilaginous and vascular calcification [2,5].
In cancer research, Mgp has been found to be involved in multiple processes. In colorectal cancer, Mgp promotes CD8+ T cell exhaustion by activating the NF-κB pathway, leading to liver metastasis. Inhibition of Mgp significantly decreased the rate of liver metastasis, and combined with αPD1, it synergistically resisted metastasis [1]. In colon cancer, Mgp promotes cell growth and proliferation by enriching intracellular calcium concentration and activating the NF-κB pathway, being associated with a worse prognosis [6]. In breast cancer, Mgp+ tumor-associated macrophages influence the efficacy of immunotherapy, with increased numbers of these macrophages upregulating pro-tumorigenic factors after anti-PD-1 treatment [3]. Also, Mgp is overexpressed in CRC and may confer resistance to oxaliplatin, and its knockdown can reverse this resistance [4]. In a study on specific heterozygous variants in Mgp in humans, heterozygous 'knock-in' mice expressing one of the variants (C19F) recapitulated most of the skeletal anomalies observed in affected individuals, suggesting endoplasmic reticulum stress-induced apoptosis of growth plate chondrocytes as the main mechanism for the observed spondyloepiphyseal skeletal dysplasia [2].
In conclusion, Mgp has essential functions in preventing ectopic calcification. In disease models, especially in cancer and certain skeletal dysplasia, Mgp plays significant roles. Gene-based models such as the 'knock-in' mouse model for Mgp have been crucial in revealing its role in biological processes and disease conditions, providing insights into potential therapeutic targets for related diseases.
References：
1. Rong, Dawei, Sun, Guangshun, Zheng, Zhiying, Tang, Weiwei, Wang, Xuehao. 2022. MGP promotes CD8+ T cell exhaustion by activating the NF-κB pathway leading to liver metastasis of colorectal cancer. In International journal of biological sciences, 18, 2345-2361. doi:10.7150/ijbs.70137. https://pubmed.ncbi.nlm.nih.gov/35414780/
2. Gourgas, Ophélie, Lemire, Gabrielle, Eaton, Alison J, Boycott, Kym M, Murshed, Monzur. 2023. Specific heterozygous variants in MGP lead to endoplasmic reticulum stress and cause spondyloepiphyseal dysplasia. In Nature communications, 14, 7054. doi:10.1038/s41467-023-41651-6. https://pubmed.ncbi.nlm.nih.gov/37923733/
3. Chang, Kexin, Jiao, Yangchi, Zhang, Bo, Fan, Pengyu, Zhang, Juliang. 2024. MGP+ and IDO1+ tumor-associated macrophages facilitate immunoresistance in breast cancer revealed by single-cell RNA sequencing. In International immunopharmacology, 131, 111818. doi:10.1016/j.intimp.2024.111818. https://pubmed.ncbi.nlm.nih.gov/38460300/
4. Huang, Chengzhi, Wang, Minjia, Wang, Junjiang, Huang, Kaihong, Yao, Xueqing. 2020. Suppression MGP inhibits tumor proliferation and reverses oxaliplatin resistance in colorectal cancer. In Biochemical pharmacology, 189, 114390. doi:10.1016/j.bcp.2020.114390. https://pubmed.ncbi.nlm.nih.gov/33359068/
5. Hur, David J, Raymond, Gerald V, Kahler, Stephen G, Cohen, Bernard A, Boyadjiev, Simeon A. . A novel MGP mutation in a consanguineous family: review of the clinical and molecular characteristics of Keutel syndrome. In American journal of medical genetics. Part A, 135, 36-40. doi:. https://pubmed.ncbi.nlm.nih.gov/15810001/
6. Li, Xueqing, Wei, Rui, Wang, Mizhu, Zhang, Shutian, Min, Li. 2020. MGP Promotes Colon Cancer Proliferation by Activating the NF-κB Pathway through Upregulation of the Calcium Signaling Pathway. In Molecular therapy oncolytics, 17, 371-383. doi:10.1016/j.omto.2020.04.005. https://pubmed.ncbi.nlm.nih.gov/32405535/
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精子検査
凍結前の精子濃度を測定し、精子の生存能力の判定します。
凍結後の精子では、各バッチから1本の凍結保存された精子を選び出し、体外受精に使用します。
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